FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_68.341000000072df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_68.341000000072df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228525
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC19890.8703642927469643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT250.0020998736.012961
CGAGCCC2250.036.00507715
CGTATGC2250.035.99719641
TCGTATG2250.035.99719640
ACGAGAC2250.035.99719622
CTCGTAT2300.035.2146539
TCTCGTA2300.035.2146538
ATGCCGT2300.035.2146544
TATCTCG2250.034.99727636
GACGACG2250.034.99727626
CGCTATA2150.034.53219631
TCTCCGA2350.034.48049511
ATCTCCG2350.034.47294610
TATGCCG2350.034.465443
GAGACGA2350.034.465424
CGAGACG2350.034.465423
TGCCGTC2350.034.465445
GACGCTA2250.033.99735329
CCGAGCC2400.033.7547614
ACGCTAT2200.033.74737530
Produced by FastQC (version 0.11.2)