##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 12h_67.341000000072c2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 65499 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.32032550115269 38.0 38.0 38.0 32.0 38.0 2 36.35672300340463 38.0 38.0 38.0 32.0 38.0 3 36.354555031374524 38.0 38.0 38.0 32.0 38.0 4 36.41689186094444 38.0 38.0 38.0 32.0 38.0 5 36.45576268339975 38.0 38.0 38.0 32.0 38.0 6 38.34153193178522 40.0 38.0 40.0 38.0 40.0 7 38.21081237881494 40.0 38.0 40.0 38.0 40.0 8 38.20636956289409 40.0 38.0 40.0 38.0 40.0 9 38.22615612452098 40.0 38.0 40.0 38.0 40.0 10 38.18465930777569 40.0 38.0 40.0 38.0 40.0 11 38.196232003542036 40.0 38.0 40.0 38.0 40.0 12 38.102077894318995 40.0 38.0 40.0 38.0 40.0 13 38.14908624559153 40.0 38.0 40.0 38.0 40.0 14 38.07386372311028 40.0 38.0 40.0 38.0 40.0 15 38.10688712804776 40.0 38.0 40.0 38.0 40.0 16 38.099039679995116 40.0 38.0 40.0 38.0 40.0 17 38.07616910181835 40.0 38.0 40.0 38.0 40.0 18 38.085344814424644 40.0 38.0 40.0 38.0 40.0 19 38.108963495625886 40.0 38.0 40.0 38.0 40.0 20 38.08882578359975 40.0 38.0 40.0 38.0 40.0 21 38.103299287012014 40.0 38.0 40.0 38.0 40.0 22 38.09079527931724 40.0 38.0 40.0 38.0 40.0 23 38.07125299622895 40.0 38.0 40.0 38.0 40.0 24 38.07615383440969 40.0 38.0 40.0 38.0 40.0 25 38.06097803019893 40.0 38.0 40.0 38.0 40.0 26 37.99239683048596 40.0 38.0 40.0 32.0 40.0 27 37.99227469121666 40.0 38.0 40.0 32.0 40.0 28 37.96606055054276 40.0 38.0 40.0 32.0 40.0 29 37.94398387761645 40.0 38.0 40.0 32.0 40.0 30 37.91902166445289 40.0 38.0 40.0 32.0 40.0 31 37.92630421838501 40.0 38.0 40.0 32.0 40.0 32 37.89601367959816 40.0 38.0 40.0 32.0 40.0 33 37.86807432174537 40.0 38.0 40.0 32.0 40.0 34 37.890212064306326 40.0 38.0 40.0 32.0 40.0 35 37.890257866532316 40.0 38.0 40.0 32.0 40.0 36 37.817493396845755 40.0 38.0 40.0 32.0 40.0 37 37.80408861203988 40.0 38.0 40.0 32.0 40.0 38 37.76277500419854 40.0 38.0 40.0 32.0 40.0 39 37.729186705140535 40.0 38.0 40.0 32.0 40.0 40 37.717018580436346 40.0 38.0 40.0 32.0 40.0 41 37.72022473625552 40.0 38.0 40.0 32.0 40.0 42 37.69243805248935 40.0 38.0 40.0 32.0 40.0 43 37.66132307363471 40.0 38.0 40.0 32.0 40.0 44 37.5932915006336 40.0 38.0 40.0 32.0 40.0 45 37.558008519214034 40.0 38.0 40.0 32.0 40.0 46 37.46125895051833 40.0 38.0 40.0 32.0 40.0 47 37.42614391059406 40.0 38.0 40.0 32.0 40.0 48 37.395013664330754 40.0 38.0 40.0 32.0 40.0 49 37.3662804012275 40.0 38.0 40.0 32.0 40.0 50 37.32078352341257 40.0 38.0 40.0 32.0 40.0 51 36.87393700667186 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.5387563957950618 2201 2 -0.06513504803866965 2201 3 -0.06912623229725057 2201 4 0.044440007697453154 2201 5 -0.33024020821788014 2201 6 -0.5923613466692146 2201 7 -0.11850683487145375 2201 8 0.19283245615211086 2201 9 -0.08124483341496358 2201 10 -0.03351152356267306 2201 11 -0.0393731398099888 2201 12 -0.49523870287975313 2201 13 -0.656465114123371 2201 14 -0.14912316694778838 2201 15 -0.27331244604600613 2201 16 -0.5910678081833609 2201 17 -0.3157078698480973 2201 18 -0.021891241548345874 2201 19 -0.6093842063293735 2201 20 -1.0503560376042955 2201 21 0.07480418523392984 2201 22 0.048557130941624393 2201 23 0.5355934469199042 2201 24 -0.7646306321305545 2201 25 -0.43948097441545286 2201 26 -0.748029407845749 2201 27 -0.3674416600605426 2201 28 -0.7308619851573184 2201 29 -0.19801143782299135 2201 30 -0.7799729556224975 2201 31 -0.6799156010235095 2201 32 -1.62661070395378 2201 33 -0.8665647756297616 2201 34 -0.7907830506177547 2201 35 -0.6069639035252266 2201 36 -0.5742422399243949 2201 37 -0.8334503104404831 2201 38 -0.35197619957492066 2201 39 -1.24778340951368 2201 40 -0.9729189787778836 2201 41 0.10505316052887537 2201 42 -1.2016817462755967 2201 43 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-0.4062551654215625 1213 30 -0.534453242360847 1213 31 -0.3802740239625777 1213 32 -1.2886178724125585 1213 33 -0.7699697935509064 1213 34 -1.25081889291166 1213 35 -0.39083487126715966 1213 36 -0.2875038886699173 1213 37 -0.2085399161752477 1213 38 -0.3700765579978551 1213 39 -0.5239482840656464 1213 40 0.2351455373511513 1213 41 -0.678997018682594 1213 42 -0.3801763699315117 1213 43 -0.41486012049327314 1213 44 -0.40725833198703043 1213 45 0.10933397005106826 1213 46 -0.024273812224350877 1213 47 -0.05805700554710569 1213 48 -0.8282886134684802 1213 49 -0.9646903574948666 1213 50 -0.4368679741728698 1213 51 -1.4766713074510704 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 0.0 17 1.0 18 2.0 19 1.0 20 6.0 21 8.0 22 26.0 23 44.0 24 87.0 25 162.0 26 248.0 27 355.0 28 532.0 29 713.0 30 918.0 31 1114.0 32 1370.0 33 1846.0 34 2215.0 35 2733.0 36 3758.0 37 5612.0 38 10321.0 39 33426.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.7997434174354 22.18370089803898 13.476693750381818 27.53986193414381 2 13.252313047726648 23.58850651928303 42.09746862499618 21.061711807994136 3 16.675063741431167 28.869143040351762 32.46767126215667 21.988121956060397 4 11.98796928197377 20.730087482251637 39.765492602940505 27.51645063283409 5 12.238354784042505 38.46776286660865 37.06163452877143 12.232247820577413 6 26.524069069756788 42.21896517504084 18.991129635566956 12.265836119635415 7 22.571336966976595 34.74098841203683 24.84770759858929 17.83996702239729 8 21.41101390860929 39.74106474908014 22.38660132215759 16.461320020152982 9 23.79272965999481 18.052184002809206 22.4140826577505 35.74100367944549 10 13.59868089589154 31.73025542374693 34.76694300676346 19.90412067359807 11 30.274206846010564 24.602277932150603 28.32605575742771 16.797459464411126 12 20.473595016717812 26.659949006855065 32.969969007160415 19.896486969266704 13 25.196189196616693 23.964090506580355 29.429906256679594 21.40981404012336 14 19.233881433304326 23.156078718759066 29.28136307424541 28.328676773691203 15 20.302600039695264 31.93331195896121 25.317943785401305 22.44614421594223 16 21.746366190301696 30.232380603395626 27.79559057041651 20.22566263588616 17 19.394485327796268 31.26049650371004 29.399370973159485 19.94564719533421 18 20.80979571895325 28.57491831811658 30.730709334636174 19.884576628293992 19 20.18046077039344 28.467610192522024 29.579077543168598 21.772851493915937 20 19.972824012580347 28.728682880654667 29.666101772546146 21.63239133421884 21 19.841524298080888 28.81723385089849 29.7302248889296 21.61101696209102 22 19.73159895570925 31.11803233637155 29.39281515748332 19.757553550435887 23 19.841524298080888 28.832501259561216 29.41418952961114 21.91178491274676 24 21.510252064917022 29.02334386784531 29.6798424403426 19.786561626895065 25 20.091909800149622 30.3806164979618 29.54854272584314 19.978930976045437 26 20.890714220281538 29.197838101926777 30.156646004458153 19.754801673333535 27 19.95450312218507 30.52718362112399 29.48441960945969 20.033893647231256 28 19.73312569657552 28.646238873875934 29.61113910136033 22.009496328188217 29 21.78506542084612 28.861509336020397 29.285943296844223 20.067481946289256 30 19.785336956853644 30.397874744267 28.965769947173957 20.851018351705395 31 20.088856318417076 28.40043359440602 29.347012931495136 22.163697155681767 32 20.9285637948671 29.107314615490314 29.446251087802867 20.51787050183972 33 19.410983373791964 28.52257286370784 30.114963587230335 21.951480175269854 34 19.97435075344662 28.846241927357667 31.337883021114827 19.841524298080888 35 20.049161055893983 30.128704255026793 30.056947434311976 19.76518725476725 36 19.42472404158842 28.97754164185713 31.423380509626103 20.174353806928348 37 19.830837112016976 29.985190613597158 29.28288981511168 20.901082459274186 38 19.62167361333761 28.31798958762729 31.18062871188873 20.87970808714637 39 19.749919846104522 27.7683628757691 29.659994809081052 22.82172246904533 40 20.04763431502771 27.57141330401991 31.669185789096016 20.711766591856364 41 19.89343348753416 27.36224980534054 30.383669979694343 22.360646727430954 42 21.440021985068476 27.659964274263732 30.2798516007878 20.620162139879998 43 19.165178094322048 27.69813279592055 32.19438464709385 20.942304462663554 44 18.74074413349822 29.06761935296722 30.598940441838806 21.59269607169575 45 19.11479564573505 27.60652834394418 32.058504709995574 21.220171300325198 46 20.872074382815004 27.102703858074168 29.92870120154506 22.096520557565764 47 18.89952518359059 27.160720010992534 30.15313210888716 23.78662269652972 48 18.626238568527764 27.60042138047909 29.820302600039696 23.95303745095345 49 20.75909555871082 27.076749263347534 30.2294691522008 21.934686025740852 50 18.577666493633394 27.88940120309017 31.402485572078536 22.1304467311979 51 18.303128387559735 27.593630242606533 30.128097470113136 23.975143899720596 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 139.0 1 78.0 2 17.0 3 22.5 4 28.0 5 31.0 6 34.0 7 35.5 8 37.0 9 54.0 10 71.0 11 88.0 12 105.0 13 180.5 14 256.0 15 278.5 16 301.0 17 313.0 18 325.0 19 301.5 20 278.0 21 279.5 22 281.0 23 323.5 24 366.0 25 493.0 26 962.5 27 1305.0 28 2059.5 29 2814.0 30 3868.0 31 4922.0 32 5637.0 33 6352.0 34 6279.0 35 6206.0 36 5825.5 37 5445.0 38 4913.0 39 4381.0 40 3938.5 41 3496.0 42 3242.0 43 2988.0 44 2974.0 45 2960.0 46 2946.5 47 2933.0 48 2886.5 49 2840.0 50 2902.5 51 2965.0 52 3361.0 53 3757.0 54 2990.0 55 2223.0 56 2035.0 57 1847.0 58 1615.0 59 1383.0 60 1284.0 61 1185.0 62 1032.5 63 880.0 64 740.0 65 600.0 66 508.0 67 416.0 68 331.5 69 247.0 70 213.5 71 180.0 72 148.5 73 117.0 74 96.0 75 64.0 76 53.0 77 43.5 78 34.0 79 25.5 80 17.0 81 14.0 82 11.0 83 9.0 84 7.0 85 4.5 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03511503992427365 2 0.0015267408662727677 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0015267408662727677 12 0.0 13 0.0015267408662727677 14 0.0 15 0.0 16 0.004580222598818302 17 0.0015267408662727677 18 0.0015267408662727677 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0015267408662727677 27 0.0 28 0.0 29 0.0 30 0.0015267408662727677 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0015267408662727677 51 0.0030534817325455353 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 65499.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.64343308496615 #Duplication Level Percentage of deduplicated Percentage of total 1 94.71724277290295 84.9077881450522 2 3.9280640789619943 7.042502988317774 3 0.794160314957221 2.1357377115780984 4 0.20598559642254224 0.7386102411748403 5 0.12365077831834438 0.5542240136042244 6 0.05772322343292047 0.31047047503545333 7 0.02759857820812089 0.17318219091879228 8 0.03833387619839907 0.2749104212702851 9 0.014403088656784644 0.11620280827991782 >10 0.08436403203852713 1.1864925764734013 >50 0.0 0.0 >100 0.0067789283140238955 0.8279726702714587 >500 0.0 0.0 >1k 0.001694731588173005 1.7319057580235633 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 1140 1.740484587550955 No Hit TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT 195 0.29771446892318965 Illumina PCR Primer Index 11 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 125 0.19084260828409594 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 124 0.18931586741782316 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 101 0.15420082749354952 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0015267408662727674 4 0.0 0.0 0.0015267408662727674 5 0.0 0.0 0.0015267408662727674 6 0.0 0.0 0.0015267408662727674 7 0.0 0.0 0.0015267408662727674 8 0.0 0.0 0.0015267408662727674 9 0.0 0.0 0.0015267408662727674 10 0.0 0.0 0.0015267408662727674 11 0.0 0.0 0.0015267408662727674 12 0.0 0.0 0.0015267408662727674 13 0.0 0.0 0.0015267408662727674 14 0.0 0.0 0.003053481732545535 15 0.0 0.0 0.003053481732545535 16 0.0 0.0 0.003053481732545535 17 0.0 0.0 0.003053481732545535 18 0.0 0.0 0.003053481732545535 19 0.0 0.0 0.003053481732545535 20 0.0 0.0 0.003053481732545535 21 0.0 0.0 0.003053481732545535 22 0.0 0.0 0.00610696346509107 23 0.0 0.0 0.007633704331363837 24 0.0 0.0 0.009160445197636605 25 0.0 0.0 0.01221392693018214 26 0.0 0.0 0.016794149529000442 27 0.0 0.0 0.025954594726637047 28 0.0 0.0 0.03511503992427365 29 0.0 0.0 0.056489412052092396 30 0.0 0.0 0.1083986015053665 31 0.0 0.0 0.2549657246675522 32 0.0 0.0 0.44733507381792087 33 0.0 0.0 0.6717659811600177 34 0.0 0.0 0.9053573336997511 35 0.0 0.0 1.14200216797203 36 0.0 0.0 1.4870456037496755 37 0.0 0.0 1.9740759400906884 38 0.0 0.0 2.6641628116459795 39 0.0 0.0 3.403105390921999 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGACG 150 0.0 40.495186 23 CGACGCT 145 0.0 40.340034 28 AGACGAC 145 0.0 40.340034 25 CGCTATA 145 0.0 40.340034 31 GACGACG 145 0.0 40.340034 26 ACGACGC 145 0.0 40.340034 27 CCGAGCC 160 0.0 39.40042 14 CGTATGC 160 0.0 39.37032 41 CTCGTAT 160 0.0 39.37032 39 TCGTATG 160 0.0 39.37032 40 TGCCGTC 160 0.0 39.37032 45 GAGACGA 155 0.0 39.188892 24 TATCTCG 155 0.0 39.188892 36 ACGCTAT 150 0.0 38.995365 30 GACGCTA 150 0.0 38.995365 29 CCCACGA 170 0.0 38.377792 19 CGAGCCC 165 0.0 38.20647 15 ATCTCGT 165 0.0 38.177284 37 GCCCACG 165 0.0 38.177284 18 CACGAGA 165 0.0 38.177284 21 >>END_MODULE