Basic Statistics
Measure | Value |
---|---|
Filename | H23NVBCXX l02n01 12h_67.341000000072c2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 65499 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC | 1140 | 1.740484587550955 | No Hit |
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT | 195 | 0.29771446892318965 | Illumina PCR Primer Index 11 (95% over 21bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 125 | 0.19084260828409594 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 124 | 0.18931586741782316 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 101 | 0.15420082749354952 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGAGACG | 150 | 0.0 | 40.495186 | 23 |
CGACGCT | 145 | 0.0 | 40.340034 | 28 |
AGACGAC | 145 | 0.0 | 40.340034 | 25 |
CGCTATA | 145 | 0.0 | 40.340034 | 31 |
GACGACG | 145 | 0.0 | 40.340034 | 26 |
ACGACGC | 145 | 0.0 | 40.340034 | 27 |
CCGAGCC | 160 | 0.0 | 39.40042 | 14 |
CGTATGC | 160 | 0.0 | 39.37032 | 41 |
CTCGTAT | 160 | 0.0 | 39.37032 | 39 |
TCGTATG | 160 | 0.0 | 39.37032 | 40 |
TGCCGTC | 160 | 0.0 | 39.37032 | 45 |
GAGACGA | 155 | 0.0 | 39.188892 | 24 |
TATCTCG | 155 | 0.0 | 39.188892 | 36 |
ACGCTAT | 150 | 0.0 | 38.995365 | 30 |
GACGCTA | 150 | 0.0 | 38.995365 | 29 |
CCCACGA | 170 | 0.0 | 38.377792 | 19 |
CGAGCCC | 165 | 0.0 | 38.20647 | 15 |
ATCTCGT | 165 | 0.0 | 38.177284 | 37 |
GCCCACG | 165 | 0.0 | 38.177284 | 18 |
CACGAGA | 165 | 0.0 | 38.177284 | 21 |