FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_67.341000000072c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_67.341000000072c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65499
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC11401.740484587550955No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT1950.29771446892318965Illumina PCR Primer Index 11 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.19084260828409594No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.18931586741782316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.15420082749354952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG1500.040.49518623
CGACGCT1450.040.34003428
AGACGAC1450.040.34003425
CGCTATA1450.040.34003431
GACGACG1450.040.34003426
ACGACGC1450.040.34003427
CCGAGCC1600.039.4004214
CGTATGC1600.039.3703241
CTCGTAT1600.039.3703239
TCGTATG1600.039.3703240
TGCCGTC1600.039.3703245
GAGACGA1550.039.18889224
TATCTCG1550.039.18889236
ACGCTAT1500.038.99536530
GACGCTA1500.038.99536529
CCCACGA1700.038.37779219
CGAGCCC1650.038.2064715
ATCTCGT1650.038.17728437
GCCCACG1650.038.17728418
CACGAGA1650.038.17728421