FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_66.341000000072b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_66.341000000072b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457732
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC8190.1789256595562469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA1300.038.07655331
GACGCTA1300.038.07655329
ACGACGC1300.038.07655327
CGACGCT1350.036.6663128
ACGCTAT1350.036.6663130
GAGACGA1450.035.6893124
TGCCGTC1400.035.36066445
CGAGACG1400.035.356823
ACGAGAC1400.035.356822
TATGCCG1450.034.137643
TATCTCG1450.034.137636
CGTATGC1500.032.99967641
TCTCGTA1550.031.93517338
ATGCCGT1550.031.93517344
ATCTCCG1500.031.49969310
CTCGTAT1600.030.93719939
CCCACGA1600.030.93719919
GACGACG1600.030.93719926
CCCCCGG300.005136065730.0095441
GCCCACG1750.029.5711418
Produced by FastQC (version 0.11.2)