H23NVBCXX l02n01 12h_65.341000000072a9.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 12h_65.341000000072a9.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2986
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	552	18.486269256530473	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	219	7.334226389819157	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	83	2.7796383121232418	No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT	57	1.9089082384460818	Illumina PCR Primer Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50	1.6744809109176158	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23	0.7702612190221032	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21	0.7032819825853985	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16	0.5358338914936369	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13	0.4353650368385801	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12	0.4018754186202277	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	9	0.3014065639651708	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	9	0.3014065639651708	RNA PCR Primer, Index 27 (95% over 21bp)
AAATAGAACCACACTGAATTAACACAACTGTGAAACTGGTATTATGAAAGA	8	0.26791694574681846	No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCTTC	7	0.23442732752846618	Illumina PCR Primer Index 11 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.23442732752846618	No Hit
TTTAGAGAACATCAAACATGTCATGTATGAAATGCTAAATCAGTTGTATCA	6	0.20093770931011384	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.20093770931011384	No Hit
ATCAAGTGTATGTTTCTCATTTTCCATGATTTTCAGTTTTCTTGCCATATT	6	0.20093770931011384	No Hit
GAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAAAA	5	0.16744809109176156	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG	5	0.16744809109176156	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.16744809109176156	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT	4	0.13395847287340923	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	4	0.13395847287340923	No Hit
GTTAAAATCCTTGTACTTCTTATTGTGGAGGAATTCACTCATGTTCACTTC	4	0.13395847287340923	No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.10046885465505692	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATTTCGTATGCCGTC	3	0.10046885465505692	No Hit
TTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.10046885465505692	No Hit
CTGAAAGACCTGGAATATGGCAAGAAAACTTAAAATCACGGAACATGAGAA	3	0.10046885465505692	No Hit
TTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.10046885465505692	No Hit
TTCAACCACTAGAACAAACTTTTTTCAATTGACAGTTGCAGAATTGTGGAG	3	0.10046885465505692	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	45	1.7644197E-10	45.0	1
GTATCAA	30	8.8741144E-5	37.499996	1
AGCCCAC	55	1.2751116E-9	36.81818	17
GTATGCC	55	1.2751116E-9	36.81818	42
ATCTCGT	55	1.2751116E-9	36.81818	37
ATCTCCG	55	1.2751116E-9	36.81818	10
CACATCT	55	1.2751116E-9	36.81818	7
GCCCACG	55	1.2751116E-9	36.81818	18
TATACAC	55	1.2751116E-9	36.81818	3
TGCCGTC	55	1.2751116E-9	36.81818	45
CCACGAG	55	1.2751116E-9	36.81818	20
TATGCCG	55	1.2751116E-9	36.81818	43
CATCTCC	55	1.2751116E-9	36.81818	9
GAGCCCA	55	1.2751116E-9	36.81818	16
CACGAGA	55	1.2751116E-9	36.81818	21
CCCACGA	55	1.2751116E-9	36.81818	19
ACATCTC	55	1.2751116E-9	36.81818	8
TTATACA	55	1.2751116E-9	36.81818	2
TACACAT	55	1.2751116E-9	36.81818	5
ACACATC	55	1.2751116E-9	36.81818	6

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content