FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_64.3410000000729c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_64.3410000000729c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC126.521739130434782No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC94.891304347826087No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC31.6304347826086956No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT21.0869565217391304RNA PCR Primer, Index 27 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT21.0869565217391304Illumina Paired End PCR Primer 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC21.0869565217391304RNA PCR Primer, Index 46 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGACGTCT21.0869565217391304No Hit
GGCAATAGGAACTAGGTATCTTTCCCCGCTACTTCTGTAAGACAGATTTCC10.5434782608695652No Hit
GGTACTGGCAGAGCTGTGGCTCGAATTCCAAGAGTTCGAGGTGGTGGGACC10.5434782608695652No Hit
ATATTATGGCATTGGTTTCACGAGCCCTGTGGACATCAAAATCTAAAGGCA10.5434782608695652No Hit
GTGAAAGAATGAAGGTAATACTCACTAGCTGCCATTCTCCTATGTCTTCAT10.5434782608695652No Hit
CATACACACATTGGTCTATAGAGAGGATAATAATCCCTGATGTAGCAGGCC10.5434782608695652No Hit
GCCCCAGCACCCATATAAAAAGGCAGGCCTGGTAGTGTCCTCTTGTGGAAC10.5434782608695652No Hit
ACATACCCAGTTCTCATCAAGGTTTCTAGAAAAATTAACCACCTGTGTCAA10.5434782608695652No Hit
ATTTGTTCCAGTGCTTTTTCATAGTTTTTACTAAGCCGGACCTGTGGAACA10.5434782608695652No Hit
AAATAGGTGTGTGCCACAATGCCCTCCAAAGCCCTGCTTAATTGTCGCATT10.5434782608695652No Hit
CCACACACACTTTCTAAATTCTCCCTCCTTTGGGCAAAAGAAAAAAGAAAA10.5434782608695652No Hit
TTGTGCCCTCTGGGAAGACATCAGCCTGATTTCCCAAGGGGACCCATGTCA10.5434782608695652No Hit
CTCTCCTCAAGCTAGGTTCCAGCATCATCGCTGTGTTATCGCTGCAAACTT10.5434782608695652No Hit
CACCATTTCACTCACTCATTCATTCATTCCCTCACTGTCTTTACTGACCAC10.5434782608695652No Hit
CTCTGGAAACAGCCAGTAAGAACTTGTCATCAGGTGAGAATCTGTCTCTTA10.5434782608695652No Hit
GATTGTTGGTCTCCAGGCATCCATCACTTAGAACCCTGGCAAACTCAACAA10.5434782608695652No Hit
GGCTGGTCTCTACCTCCTGAGCTCAAGTGATCCACCTGCCTCGGCCTCCCA10.5434782608695652No Hit
GGCCTGATGACCCACTCCTGCTGCTGCTGCATGGCTAGCAGATACTGCTTG10.5434782608695652No Hit
GATGGTCCTTGACTTCCTCACAAGGGATGTGTCGAATGATTTGACAGTCAC10.5434782608695652No Hit
GTATTAACAGTTAAATAATCCCAAAACTCACACAGACCTTGGAGATATTCA10.5434782608695652No Hit
CTAAAGAACTCGGCACAGCATTTGTTGCAGATGTGAGTGTCCTCCCGCCGG10.5434782608695652No Hit
AAGCCAAGAGAGAAAGCAGAGTTGGCTCTGCTCTGGCAATGAGAGGAAAGG10.5434782608695652No Hit
CAACAACGGCCGCCACCACCGAATGGACGAGTTCTCCCGCGGGAACGTGCC10.5434782608695652No Hit
GCTACTAGGAAGATATTTTTCCCTTTCAGGCCGCTTAATTTTGATGGATAT10.5434782608695652No Hit
GCATTTCAAAGTAAGGTTTGTATAAGCCACCCTCATTCCCCTTTTTTGGTT10.5434782608695652No Hit
CCACCAGGCTTATCGCAGATTCCCACGTCCTTCTTCGACTCCTGTTGCCAA10.5434782608695652No Hit
GTATTTGCATGACACCTTATAACTTAGTGTTTCAGCACACTGTAATGTAAG10.5434782608695652No Hit
CATTTGAGGGTGAACAAGTAAAAAGGTGTCATGAAAAGCTTTCTACATTTT10.5434782608695652No Hit
ACAGAACAGAGGGTTTGCATTTGTGCAGCTATCTTCTGCAATGGATGCCTC10.5434782608695652No Hit
TCTGAATAGGTTTGGCATGGACAAGATCTATGAAGGCCAAGTGGAGGTGAA10.5434782608695652No Hit
GCTATGATCTGTCCCAGACACAACAGCCCCAAGCCCCTCACACCTCACTAA10.5434782608695652No Hit
GGCTATAAGGGTTCCTCCTTTCACAGAATTATTCCAGGATTCATGTGCCAG10.5434782608695652No Hit
GCCCCCACCTTCAGTGCTGGAGGCTGACCTCACAGAGGGAGACCTGGCCAA10.5434782608695652No Hit
GCAAAGGGCGGAGGCGCCCTTGCTGGATCCTCAGAAGCCAGATCCTCTTGG10.5434782608695652No Hit
CACATTCTCTCAGCTTTTTCTGAGCCCCGTCAAAATCAAAGTTAACATATA10.5434782608695652No Hit
GGCTGATATTACAGTTGTCTGTACCCTGTTGTGGCTCTATAAACAGGTCTT10.5434782608695652No Hit
CAATAATGCATTGCCTCTATCATGTTAGAACGTGCATTAGACTCAAATACA10.5434782608695652No Hit
ATTTTAGGCATTATCCTCACAGAACAACTGATTTCTCACAAGCTCAATATT10.5434782608695652No Hit
CTCGTTCTGTTGCCCAGGTTGGAGGGCAGTGGCGAAATCTCGGCTTACTGT10.5434782608695652No Hit
GCCCATTGGTGACCACATTGGGTGTAAAAAGCCTCATTGAAAGCAAAACCG10.5434782608695652No Hit
CCACAATCTTCTTCGTTGTCTTTATAGGTGTGCTGATGATGGAATACTGCC10.5434782608695652No Hit
CAATGCAAACTGTGCACTGGATGTAATGTCTTCACGCTGTTGGTCAGTCAT10.5434782608695652No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACA10.5434782608695652No Hit
GGGCAGAGACCTTCCAAAGTGAAGGCAGAAAGTGTGGCCCTGTCTCTTATA10.5434782608695652No Hit
GGCAGGGGCCTGGTGGGGCTTGAAGAGTTTAGAGAGGTCCTCCTCAGGGAG10.5434782608695652No Hit
ACAGAAGGTTAAAAGATTATGTTCACCTTGGGTTTAGTCTCTTTTAACACC10.5434782608695652No Hit
CTGCCTGCCTTAGCCCAGACTTGGTGATTCTGGGGGAGGTACTGCTCATTA10.5434782608695652No Hit
CATTTCAACAGACTTGACTTCAGTTGTTACATTGACTGGAGCAAAGGTAAC10.5434782608695652No Hit
CTTATACAACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT10.5434782608695652No Hit
ATTGACGAGTACTGCGTGCAGCAGCTCAAGGAGTTTGATGGGAAGAGCCTG10.5434782608695652No Hit
TTTGTGCAATGTGTTATTTTGTTGATCTGTGTACATTTTTCATTTCATGTC10.5434782608695652No Hit
GTACAGAAAGTAAAAATGTTGTTACAATCGCAGTGTAACTGGCAGCCAGGA10.5434782608695652No Hit
GTAATGACAGCAGCAGCATTTAGAGACACTAAAGATTAACACACTCTTATA10.5434782608695652No Hit
CAGCTATACTATGAGCCTTAGGCTTTATTTTCTTATTTACAGTTGGTGGTC10.5434782608695652No Hit
GTATGTGTAAGGTGCAGGAAAGGGAGAGAGTGCATGGCAAGAGGTGTGAGC10.5434782608695652No Hit
TGGTAGACTTCATCTTTATTGTGTACTGTGATTTCCTCTAAGCCTTTGATT10.5434782608695652No Hit
CTCCTGCAAGGCACCCACAAGAAAAGGTTCTGCCTTCACCCAGAGGAGAAC10.5434782608695652No Hit
ATTAAAGTCCTTCTTGATGTCCTCTACCATCATCATGAGATATGCTTTTAA10.5434782608695652No Hit
GTCTTGCATAGAACTGAGTGTGTAGGTAAAACATGGCTAGAATGACAGCTG10.5434782608695652No Hit
GTTGTACTTCTCATCACTTCTAGTTCCCATGGTACCTAATGATTGCTGTCT10.5434782608695652No Hit
CTATATAAGTAATGAGTGTAGCCTAATTTTCTGTCATGTGCTCCCTAGCAT10.5434782608695652No Hit
GTAGTAAGTAACTACATGTGAAATAATTCCAAATCCTGGGAGGATAAGAAT10.5434782608695652No Hit
TCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCTTCTGCAAGA10.5434782608695652Illumina Paired End PCR Primer 2 (96% over 26bp)
AAGTAGCAGACTTTTCTGTGTAAGCAGCCCCTGGGAGAATAACATCAGCCA10.5434782608695652No Hit
CTGTGTGTCAATCCTCCCATAAATACTTTTAGCAGGTGTTCAATTATAGTC10.5434782608695652No Hit
GAGCAGAAACCCTGAAAGGTGGCCAGCCACCCTGGGACAGTGAAGTCACCT10.5434782608695652No Hit
ATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCTTC10.5434782608695652Illumina PCR Primer Index 4 (95% over 23bp)
CCATTACCCTCGAGTCAGAGTGCGAGTCCTTTCCTAGTCTGTCTTCAGATG10.5434782608695652No Hit
GCTACAGCTAGGTCCATAGAATTATAGCAAGAGTCTGTTTCAATTGTTGGG10.5434782608695652No Hit
AATGAAAGAGAGGCGAGGAGAGGAAATGAGAATGTGGCAGAGAGGGGAAGC10.5434782608695652No Hit
GAAAAAGGCTGTAAATTAAAAAGGAAACACAGAAAATACTGCTTTCATAAA10.5434782608695652No Hit
CCACATGAACATTGGTACCATTGGTCACGTTGACCACGGTAAGACGACGCT10.5434782608695652No Hit
GTTTTTAGAGACAGGGTTTCTCTGTATATCACTGGCTGTCCTGGAATTCAC10.5434782608695652No Hit
CACACCACACACAGTGTCCTCCCTCACCACACACAGTGTCCTCCCACACCA10.5434782608695652No Hit
TCATCATCTTGTGCATGGGCAGGTTATAGTTACTCTTGAGGGAAGCAGCCT10.5434782608695652No Hit
CATATGTACAGCTAAGCAGCCTGATTAGGCTTCCTTTAAGGTCACATAAAC10.5434782608695652No Hit
ATACTAAAAGCGGTAAGGGAAAAAGGTCAAGTAACATACAAAGGCAAGCCT10.5434782608695652No Hit
CAAATATACTGTACTTATGGACCAAATAAATGATGTCTGCATACTTGGCCT10.5434782608695652No Hit
GCCTATATCCATCATCACATGAAGAATGTCATTCTGTTAACTTGCCTGTGT10.5434782608695652No Hit
ATGCACCTCCAGTGCAGTCAGACATCAGATTCCCTGGAATTGCAGTTAGGG10.5434782608695652No Hit
GAAGCACTCCCCCATATTAAAAAGAGCGGAGATATTAAAAGAGAGGCGAAC10.5434782608695652No Hit
CTTATACACATCTCGGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC10.5434782608695652No Hit
ACATTAGGGTTCCAGAACCTTAATTCTGAAAAGTTGTCACTAAGCCAGCTT10.5434782608695652No Hit
AGTACTGCCTGCTCTTCTGGGACGCCAGCATCACATGGCAGCTAACAACTC10.5434782608695652No Hit
CATTTTTTCAGGTGCTTCTCTGCCATTCGGTATTCCTCAGGCTGTCTCTTA10.5434782608695652No Hit
CTTATACACATCTCCGAGCCCCACGAGACCATTCCGAATCTCGTATGCCGT10.5434782608695652No Hit
GGGCAATATCAGACTCCCATACATGTAATTGGAATACCAGGAGAAGAAGAT10.5434782608695652No Hit
TCTTCAAGCTCTCCACCACTCCTGCTACGCTCTCATGCTCCAGGCTGCTGT10.5434782608695652No Hit
CCATTGTCTGATTTATGGTCAGTGGGTTTAAAAAGAAATCCACATGCAGAT10.5434782608695652No Hit
GTGGTTTTCCTGAAGCAGCTGGACAGTGGCTTGCTGCTTGTGACTGGGCCT10.5434782608695652No Hit
ACCTGCAGAGGAGAACTTGATATCAGCTACTGCTTCAAATTTTCCACCTCC10.5434782608695652No Hit
AACAAGAAGTGTGGCCTGGAATCTAAAAACAAATAAAGGGAGAGAGCCAAA10.5434782608695652No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCCTATCTCGTATGCCGA10.5434782608695652No Hit
ACTGATGAAAAGGCTGTTATTGTATCTGATGTGTAGTGTATGGCTAAGAAA10.5434782608695652No Hit
GTATATCCCTGGCTGTCCTGAAACTCACTTTGTAGACCAGGCTGGCCTCCA10.5434782608695652No Hit
GAGTGTGTCTATATCTTCCAGACCTCATTGTGCCATGTTGCTAATCAGTGT10.5434782608695652No Hit
GTTCACTACCATTGCACACAGGAAGAGGACTCAGACAAGGCCATTCCAGTG10.5434782608695652No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT10.5434782608695652No Hit
CTTAAAAGTTGTACTTGGTACCCTGGAAACACAGGCAGAAAGGACAGTGTC10.5434782608695652No Hit
AAAATGCACAGTACAAAGATTATTTGCTTTGATGGGATCATGAAGAACAGA10.5434782608695652No Hit
GCCTGTGTGTACCTTAAAATCTTGCAGAAAAGGAAGTATAGATTGAGGGAT10.5434782608695652No Hit
CCCAAGGACCTGCCGTACTGTTGAGGTGCTGTGTGCACAACCCCTGGGGCT10.5434782608695652No Hit
CCATCAGAGAGCTCACACCGGAGAAAAGCCGTTTAAATGTGACCAGTGTGG10.5434782608695652No Hit
GGTGTACTCTGTTCCCATGGGGGGTATGATGGTAAGACAGTCCTTTGCCAT10.5434782608695652No Hit
TCCCTGCTTAACAGGACCCAGAAAAAGCAAACTAACCTCAAAGGAACAAAA10.5434782608695652No Hit
ACCTTAAGGAGGGTCTTACAGCATCTTATTTACATTTTCAACTGCAACAAA10.5434782608695652No Hit
GTAACAAACTCTAGTACTTTCAAATCCAGCACCTTTCAAATGTTTCACAGG10.5434782608695652No Hit
GAGTTCATGTGTGTCAAATGTGTGCAGCTACCAGAGAAGGCTAGAAGACAG10.5434782608695652No Hit
GTATATACCCAAAGGACTATAAAATCATGCTGCTATAAAGACACATGCACA10.5434782608695652No Hit
CAAGATGTCTGTCTTCCTTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCT10.5434782608695652No Hit
TCCTCAACTTAGTGGCAAAGCCCTGTATGGATACTTGTAGGTGCGGGTGAT10.5434782608695652No Hit
CTCCTGTGTACCGGATACCGATTACTTCGAATCCCAGTACTTTGTACTCAG10.5434782608695652No Hit
TGTCAATAGTGATACCACGCTCACGCTCAGCTTTCAGTTTGTCTAAGACCC10.5434782608695652No Hit
ATATTTTAATCATGAAAGGTACTCTAAATGATGACTGAGGAAAGGACTGCC10.5434782608695652No Hit
CAGCAGTTCAAAGCAGAGAAACTCAAAGAGAAGCTAAAAATGAAAGAGCGA10.5434782608695652No Hit
GTGTACCACTGTGGATGAAGCAGAAAAAGCAATTGAACTACGTCTGGCAAA10.5434782608695652No Hit
ATGAAGCTTTGAGTGAAGCTCTTCCTGGGGACAATGTGGGCTTCAATGTAA10.5434782608695652No Hit
CTCCACAGGCCAAGCGGCTCCCAGCATTTCCAGTCTTTGTACTTTCTTCAT10.5434782608695652No Hit
CTCATCACCTTGTCACTCCAGTGATCATACGGATACACATCTAGGTAGTTA10.5434782608695652No Hit
AGGCAACTGTTTTCTTAGTTACAAGCTTTACTTTTGTGCAGTTAAAACAAA10.5434782608695652No Hit
GTCTGGTGGCTTTCGATTTTTGGCGTTCAGTAGTCGCGGTTGGTGGGTAAC10.5434782608695652No Hit
GTTTTATGCAATGGCAATTAAAGTTTTTCTTCCACAAGTTGAGTCTTTGTA10.5434782608695652No Hit
CTTATACCACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT10.5434782608695652No Hit
GTTTAGAGATTGTTCTTCTGGTGATTCTGTTACCGTCTATCAGCAGACCTG10.5434782608695652No Hit
CTTCTGTATGGACACAGTAGAATATAAAGTGACCAAAGTAGAATTCAACAT10.5434782608695652No Hit
CCTGCTTTCTGACCTGTTTGAGTTCCAGTCCTGACTTCCTTTGGTGATGAA10.5434782608695652No Hit
CTTTTTTAACCGTCTCTGCCGGGCCTCCTTCTCCATACCCATCAGCTGACT10.5434782608695652No Hit
CTGGAACCTTGTAGCACTCATAACTCTCGAAGGAAGTGCCCCCCGGAGAGT10.5434782608695652No Hit
CTATCAATGGGAGCAGTGTTTGCTATCATAGCAGGATTTGTTCACTGATTC10.5434782608695652No Hit
CTCTGGGAAGTTCTCAGGAAGATCCATCACAGCAGCAAGGAGCCTTGCCGC10.5434782608695652No Hit
GACTTCCATTCTGAGAAGCAGGGGAATCGACGTGGTGTCACCATTTAAATG10.5434782608695652No Hit
TTGTTGTTTTGTTACAGGTATGAGCTGCCATGTGAGTGCTGGGAATTGAAC10.5434782608695652No Hit
GAAAAGGCACTTAAAACAAGATTGTGACTAGTTCGAGATAGAGCAAGCATG10.5434782608695652No Hit
TGTTAATACTGACTTGAATGGGGACCTTATGGTGAATCCTAGACTATGAGG10.5434782608695652No Hit
GTTCCCAAAGATGTCAATGCTGCCATTGCCAACATCAAGACCAAGCGTACC10.5434782608695652No Hit
GAGTGACTTTTATTTTTTGTTCATTGTTGGTGGTTTTAAAAAAAATTCCCC10.5434782608695652No Hit
GTCCTATTTAAACTTGTGAGAAAAAGTGGTATCCCTTGGTACCTGTCTCTT10.5434782608695652No Hit
TGTTTCTACTGACCTGAATGTGTTGGATCTTCACGTGTCGTTTTGTTTTTG10.5434782608695652No Hit
CTCAGGTACTGTGCATGGCCCAGTTGCTGGTACTGAGCATGGCGCTGACGC10.5434782608695652No Hit
CCTAATGAAACAAACTCCAGAAATAACATTCGGAAATCTGTTTTCACAAGC10.5434782608695652No Hit
GTGTAATAGAGGGATTTCTTTATCGTATGCAGTCTTCTGCTTGAAAAAAAA10.5434782608695652Illumina DpnII expression Adapter 2 (95% over 21bp)
CTGTAATGTCCAGTTTTATTTACGGGATACAACCATGCCTGTCTCTTATAC10.5434782608695652No Hit
ACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTTCTG10.5434782608695652RNA PCR Primer, Index 46 (96% over 26bp)
GCACGTTGTGAGCTTCAGGGAGCAGGCCCTATATCGCAGGGAGTTAGGCTC10.5434782608695652No Hit
ATCCATCTAGACAGATTTTGGTTACACTTTGCCTATTGATGGAGTAGTTCC10.5434782608695652No Hit
GTCCTGCACTGGTACAGCCTTGTGTATTGTCCCCATACTGATGGACGTGGA10.5434782608695652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA10.5434782608695652No Hit
CCTCTATTGTTTATGCCCATAGCACCTCCCATAGCCATTTGGCCCATCCGT10.5434782608695652No Hit
GCATGCAGAGGAACGATGAGAGCCCGGCTCCCAGGCCCCACTAAACACATG10.5434782608695652No Hit
ACTGACAGTTGAGTTGATTCTCTTTTTACAATAAAAAAGCTAAGTAATATT10.5434782608695652No Hit
CTTAGAGTGAGGATTAAAATGAGGTCCTGCACGGGTACAGCCTTGTGTATT10.5434782608695652No Hit
TGTTATTAGAGCTCTGGGCAGATGCTGTTATTCTCATTTTTAAAGTGTTCA10.5434782608695652No Hit
TCCTATAATCGCAGCACTTCGGAGGCAGAATGAGGGAGATTAGGAGTTCAG10.5434782608695652No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers