FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_63.3410000000728f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_63.3410000000728f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312964
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC18500.5911223016065745No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3320.10608248872074744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGCC250.002104881235.99961532
GCCCACG2950.035.84707318
TATGCCG2950.035.0843743
ATGCCGT2900.034.9134244
AATCTCG3000.034.49963436
ATCTCGT3100.034.11253737
TCGTATG3100.034.11253740
GGCGAAT3050.033.93406732
TGCCGTC3000.033.7496445
CGTATGC3150.033.5710741
CGAGACC3100.033.3867423
ATCTCCG3250.033.2304110
GCGAATC3150.032.85679233
CAAGGCG3150.032.85679229
CTCGTAT3250.032.53811339
CACGAGA3250.032.53811321
TCTCCGA3250.032.53811311
GTATGCC3200.032.34340742
AAGGCGA3350.032.23846430
CCCACGA3300.032.04511319
Produced by FastQC (version 0.11.2)