FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_62.34100000007272.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_62.34100000007272.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC2715.517241379310345No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC116.321839080459771No Hit
CTTATACACATTCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT21.1494252873563218No Hit
TTCTGGGGCTCCCACAAGGGGAGATGGGAAGAGGTTCCACATGTTGGGCTG10.5747126436781609No Hit
AACTCAGAGGGGAACCAGAGCAAGCTCCTTTCTGGCAGCTAGTAATTAAAT10.5747126436781609No Hit
CTTTAGTACTTATTCAGCTAAAGACAATGATGCGTCTATAACACAAGAAGC10.5747126436781609No Hit
TCATGTGAGTAAGGTAAAGAGAACTGAACCCTAGTCCTCCAGAAGAGCAGC10.5747126436781609No Hit
CTCTTACCCACTCCCCAGCCTGGTTAGCAAGCTCTAAAAAGCTTCCAGGGA10.5747126436781609No Hit
TTATACACATCTCCGAGCCCACGAGACAAAATAATATCTCGTATGCCGTCT10.5747126436781609RNA PCR Primer, Index 44 (95% over 22bp)
GTCCTGCTCCACCTTCTTCAGGTTCCGCCCTTCCTTGCCAATGAGTCGCCC10.5747126436781609No Hit
CCCAACATTGCAAATCCATTCTTGTTTTAAGCTATTAATGACACTGCTTTT10.5747126436781609No Hit
CTGTTTCTGTTCTGACGTATGATAGACAACCCAGGCTTTCCCTGTTCAAAA10.5747126436781609No Hit
ACACACACAATAACGCATGAGGTTATCAAAGTCCATGCTAATGAGTGCTGT10.5747126436781609No Hit
GCATATAGCTTTCTGTTTTGTTTGTTTCTTTTTCTTTTTTCTTACTGAGAT10.5747126436781609No Hit
GCTCTGGTAAGGAACATACCCTGCTCTCCTGTTTCTCATTGTGCCCATCTT10.5747126436781609No Hit
CCTATGAACTGCGAATGTTTCTTCACCACTTCCTTGACCCTCCTCTCCTCC10.5747126436781609No Hit
ATCACCAAACTGCTTACTCTGTGATTCAACATGTCACTTTACTTTGAAAAT10.5747126436781609No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTGGACTCTCGTATGCCGTCTT10.5747126436781609No Hit
CAATGTGCGTGCCACGGGTGGCTGAAAGCCTGAGAGGTCAGCAGCTCCTGA10.5747126436781609No Hit
GCTCTGCAGAGAACTTAAAATCATCCATATTGCAGACAAAGTACTGCCCAT10.5747126436781609No Hit
CTTATCTACAGCTCCAGGAAGATCTGGAAATAACTAGTTCTTCCAAGAACA10.5747126436781609No Hit
CTTCAGTTCTGCTCTGATCTTAGTTATTTCTTGCCTTCTGCTAGCTTTTGA10.5747126436781609No Hit
GTTGTGCCCATCCTGGCAGACTTAGTTGCCGATCACCTTGTTGTAGCCATT10.5747126436781609No Hit
CAATGTCATAGCTTCCTCCTTGACTCTCATTTCTTTAGGAAAATGAGCCAT10.5747126436781609No Hit
ATCTGGAACTCAAAGGCAGCCTCTGTCACAGAATGAATTTGAGGCCAGCCT10.5747126436781609No Hit
GAGTTCAAGTCCTAAATATCCTTGTTAATTTTCTGTCTCGTTGATCTAATA10.5747126436781609No Hit
GTCTACACACGTGCATACATACAGGGCAACTTTCCCTGTAGAGTACTCTTT10.5747126436781609No Hit
CCCCTCATCACTTGCAGCTTGCTATACGAGGAGATGAAGAATTGGATTCTC10.5747126436781609No Hit
CTCAGAGACCACGGCTGGAGTAATAAGCTTATAGTTCGGAACCTCCTTACA10.5747126436781609No Hit
GTCTTCTAAGTCTGGCAACTCTTTCTGAGGACAAGAAAAGGCAATTCTTCC10.5747126436781609No Hit
GCTTAAAAGAGATCCAAAGAGAGAAAGAGCAGTATGCCACTTCTTAGAACC10.5747126436781609No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT10.5747126436781609RNA PCR Primer, Index 44 (95% over 22bp)
CTAAAGTGGTAGGATTGCAGTTTTGAGCCACCAAGCCCAGCCAGACTTTTT10.5747126436781609No Hit
GCCACTGGCACTGCCATCTTTGCGGGTGACTTTCCATCCCTTGAACCAAGG10.5747126436781609No Hit
GTGTCATCAGAAGATGGAAAAATCAGAAATTAAGTCTTTTGTCACATAAAG10.5747126436781609No Hit
ACCTTATCTTCAGATACTTGCAAAATAGATCTCGAATTCAGGTGTGGCTGT10.5747126436781609No Hit
TTTCTATTCCATTCCATTCCGGATGATTCCATTCCATTACATTCCATTCCA10.5747126436781609No Hit
GGCTTCATCCACATATTTTTGGTAATAAGCTACCAGGGCCTTTGAGATGGA10.5747126436781609No Hit
GGATGGTCCAGCGTTTGACATACCGCCGTAGGCTTTCCTACAACACAGCCT10.5747126436781609No Hit
CCTTGTGGTTTGGGTATATTGCCAAAAGGTAAACCAGCCACGACTTCATTA10.5747126436781609No Hit
GGATACCAGCTCCGAGATCACCACCAAGGACTTGAAGGAGAAGAAGGAAGT10.5747126436781609No Hit
GCTAAATAATAAGCCAAAGAAGTGCAATCTGTAAGGGGCAAAAGCCCAGGG10.5747126436781609No Hit
GTAGGAGAAGTCCATGATCCTCACAGCGTGATCGTCCTTATTTACATAGTC10.5747126436781609No Hit
CCACAGCAATCAGGCAAGAGAAAGAAATGAAAGGCATCCAAATGGAAAAAG10.5747126436781609No Hit
GCATTGGAGTGACTGAACAACAAGGCTGTCACCAAATCCCACACATTTTAT10.5747126436781609No Hit
GTTCCTCTCTGCTGCCCAGTCACGTTTTCTTTCCCGCCTCGCAGTATCAGA10.5747126436781609No Hit
CCTGTGACCACTGTTTCAATGTTTTCCTTTTTGTTTGTCTCCCAGGCAACT10.5747126436781609No Hit
TATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCAATAAG10.5747126436781609No Hit
TTACATCATTGTATGTGATCACTTGTCAGTGTTCAGGTGTATTTTAGCAAA10.5747126436781609No Hit
CATCTCATCAGTATTTCTAGATCTTTTAAAGGCTTGCTCCTCTTCCATATC10.5747126436781609No Hit
CTCTACAATGGGCTACATGATGGCCAAAAAACACCTGGAGATCAACCCTGA10.5747126436781609No Hit
GGCTAATGGCACAACCGTCCATGTGGGCATCCACCCCAGCAAGGTCGTTAT10.5747126436781609No Hit
CTGGAGCACAGTGGTGCAGTCTAGGCTCATTGCTCCCTCTGCCTCCTGGGT10.5747126436781609No Hit
CACAGAAATCAGGAGGTGCCGACCCAAGTCTTCAGCAAGGAGAGGGGAGTA10.5747126436781609No Hit
GTATACTGTAGCTGTCTTCAGGCACACCAGAAGAGGGTGTCAGATCTCATT10.5747126436781609No Hit
CTCGAGATCTGAGGACGTCCTTGATTATGGGCCTTTTAAAGACATCCCTGC10.5747126436781609No Hit
GTCCAGTATAAAGATTTGTATAAAGAGCTTACATTTTTGTGCATAAAAATG10.5747126436781609No Hit
GGGCAGAGATGGGCTTGCTGGGCTTCCAGACTAGCTGAGTCACAAGCCCCA10.5747126436781609No Hit
ACCCAGTATAGTATATAAGAATGGACAGGAAGGTGAGAAGATAGTAACGTG10.5747126436781609No Hit
CACCAGAAGAGGGCGTCAACCAGAAGAGGGCGTCAGATCTCATTACAGATG10.5747126436781609No Hit
GTGTTAGATAGAACCATCCAAGGAGCACGGGCACCACAAATCCCATGTGTC10.5747126436781609No Hit
ATACAGTTTTCAGTTGCTTGGAGGGGTTTGCTATTGAGAAAAAAACTGTCT10.5747126436781609No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCAT10.5747126436781609No Hit
TCCTAACTATGTGAAGTTTGCAATTATCTCCTACACCTGTCTCTTATACAC10.5747126436781609No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5747126436781609No Hit
TTCCACGCCTGTGCCATCCATCTTTCTTCTGAGGCAAAGACCCTCGAGACA10.5747126436781609No Hit
CCAACAGTGTGGCTTAAACATTTGTTTCTTGGTGTTGTTATGGCGAGTTTT10.5747126436781609No Hit
GGCAGATACAGTGTATCTATGTACTATATGTGCCCTGTTGTCTTGGAATAC10.5747126436781609No Hit
GTATGAAGGAAAGGTATTAGGGCTAAAATTAGGATAGATAAGATCTGTCTC10.5747126436781609No Hit
TACTTGGAATGACTATTAAAAAAAAAGACAAAACCAAAAAAAAAAAAAAAA10.5747126436781609No Hit
GAGTTTGTTAAAACACGCTGTTCTGGTTGATTGCAGAGTGTGATTTCTGTC10.5747126436781609No Hit
GTGTGTTGAGGCTAAGTTGGTGGGTTGGGTTGGCTTCTTAACATCAAGAAA10.5747126436781609No Hit
GGAATACAGATTAAAACCAAATATAGTTTCTCACACATTGCTGGTGGGAGT10.5747126436781609No Hit
CTTATACACATCTCCGAGCCCCCGAGACCCTGGATAATCTCGTATGCCGTC10.5747126436781609No Hit
TCACGAAGATGACAGATTGAAAGCAAAGTGAAAAATTTTGAATGTCAGCAA10.5747126436781609No Hit
ATCCTACACCGAAAAACTTTCCACCAAACCCCATAAGGAGAAAGGTCATAT10.5747126436781609No Hit
CTATTATACACTTGCCGTTTTCGTCTTCAACTTGGTATCGTGCATCATCTT10.5747126436781609No Hit
GCACCATGGCGGTCGGCAAGAACAAGCGCCTGACGAAAGGTGGCAAGAAGG10.5747126436781609No Hit
GTCCCTTGCCTGCAAAGTCTTCTGCTTCCAAGCCTAGCACTTGGTAAATAT10.5747126436781609No Hit
TGATAAAGCTGACCAGAGTCTCCTTTTCTGAAAATGCATATCCATTTTACG10.5747126436781609No Hit
GCTATATAGAAACCCTGTCTCGAAAAACCAAAAAAAAAAAAAAAAAAGCCA10.5747126436781609No Hit
GTATGAGTCCAAGAAAGCTAAAAAGCCTGCAGTTGTTGCGAAGTCTTCCAT10.5747126436781609No Hit
GCTGAGACCTTTCTCTTGGTATTTTTCTGAAGGGCATGTCAAATAATTTAA10.5747126436781609No Hit
TCTGTAATGTTCTGGTACAGTTGCCAAAGTTTAGTGCTGTCTCTTATACAC10.5747126436781609No Hit
AAAACAAACAAAACAAACAAAAAATCACCTAGTTCAGGAAATCTAACATTT10.5747126436781609No Hit
CTACAGCTGCACCTTATGTATTTCACGATGATCCTTACCTCATACCAACCT10.5747126436781609No Hit
GGATTTGATCCAAATCAAATAAGTGTAGCCACATTACTGTTCGAGGGAGAC10.5747126436781609No Hit
GCTCAGGGAAACTCCTTACAAGAAACCCCGCCCAGGGAAGCAAGAAGGAGG10.5747126436781609No Hit
GCTTTGCATCCTGAGCTGAGATGCGGTCACCGGTGAGGATTTCTGGCTGTG10.5747126436781609No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC10.5747126436781609No Hit
CAACAAATGTTACTTACATCATAACGGAAAAAACAAACATTTCTCACCACC10.5747126436781609No Hit
CAAAGTAGAGAGCACGTGGTTAGAAACCACTTCATCAGAATGTGTGAGTCC10.5747126436781609No Hit
GCAGAAGTGAAGTTGAAGTAGCCAGCTTTGAGAGGACGCAGGACAACTGTG10.5747126436781609No Hit
CCAGAATATTGAACTAGGTACGAGTTATTTGGTAAAGGAACTGTCTCTTAT10.5747126436781609No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCACGTATGCCGTC10.5747126436781609No Hit
CTTTAGGACGTGAAATATGGCGAGGAAACCTGAAAAAGGTGGAAAATTTAG10.5747126436781609No Hit
ATGTATGCTACCATCCGCACTGAGAGTATTCGGTGGCTGATTCCAGGACAA10.5747126436781609No Hit
ACCTAAAAGGGCTAGTAGAATCCGCAAGCTTTTTAATCTCTCCAAAAGCTG10.5747126436781609No Hit
TGTCTATCATAAAGATCAAGGTATTTGAGAGGTGTTTCCCAGTTAACAAAG10.5747126436781609No Hit
TGCTAAGTGAGTGAACAAAATTTGCTAATATCAATTAATGACCTGCATGCC10.5747126436781609No Hit
CTGCAGTACAGTAGCAGTGTCCTTTTCTGAGGAAGGAAGTCTTAAACCCTT10.5747126436781609No Hit
CGTTTATTGAGTACACATGATAATGGATACATAAGCTTCATTCCCATCTGT10.5747126436781609No Hit
ATCTAAAACACACATTGCAAACATACAAATATCTATTCTCTCCACATGTCA10.5747126436781609No Hit
GTGTATACAGCAACTATTTGCAATACTTGGAAAGTAGACAACACAGGCTGC10.5747126436781609No Hit
CTCCGTCTGCATCCCTTGCTGAACACCAAGTTAAGTTAGTACTGGCCCACT10.5747126436781609No Hit
CTATACAGGAACGATCCAGGAAGGGAACTTGTGACTCAGGGTTCCATCTGG10.5747126436781609No Hit
GAATTGAGCCTATGTGTAGAGAGAGCTGGGCTGTATATCAGGGAGGAAAAG10.5747126436781609No Hit
GGTTCAAGCGATTCCCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGT10.5747126436781609No Hit
GAATTTTAGCTCTATTTTACAGCATTAAAAAGCTTACGCATCAGGTAGCTT10.5747126436781609No Hit
GGCTCAAACCGTCTATTTTGGAAAAGGTTTAGAATATCTACCTGTCTCTTA10.5747126436781609No Hit
CATCACGTCAAACTTTGTCTCCCCAACTTCGTATTTTTTGATACGCACTTT10.5747126436781609No Hit
GTCGTATCCACCAGTGTATCAGCATTAATATGTTAGCAGATAAACTGAATA10.5747126436781609No Hit
GCCTTTGCATTGTTGGCCTGCATCTTCTTCAGGCCTTTCTTGTTGTGCTTC10.5747126436781609No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGC10.5747126436781609No Hit
CCCAGACATGGAACAAACAACCCAGCAGGTTATCAAAACGACGAAGTGGTG10.5747126436781609No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCTTC10.5747126436781609Illumina PCR Primer Index 8 (95% over 23bp)
GAAGAAAGTTACTTAACCAGCAACTCACCCCTGGAAAATGAACAGAGCCAC10.5747126436781609No Hit
CTTTCAAACTGACAGACAAGGACGGGGAGTATAGTTAACTCGTAAAGTGTA10.5747126436781609No Hit
CCCCAGGGTCGTGGGGAGTGAGCACAGATAACACTGGACTGAGTTGATACC10.5747126436781609No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT10.5747126436781609Illumina PCR Primer Index 8 (95% over 21bp)
GTGTGTAGTTATGTATGTGATTATGCTCTATACGAGTTAGTGTGAAGATGG10.5747126436781609No Hit
GGCTGGAATGCAGTGGCAGGATCTCGGCTCACTGCCACCTCCACCTCCCAG10.5747126436781609No Hit
GTACATGCCTGGCGGAGACCTTGTCAACCTGATGAGCAACTATGACGTGCC10.5747126436781609No Hit
AGCTAGAACCCCTCCCTGCCCCAATCCTTTGAGTGTCCATCACCACAGACC10.5747126436781609No Hit
GTCTTGCTGCAGGTAACTAATGAAGAAGTGGTCAACCACAGAGTCTTCAAG10.5747126436781609No Hit
ACACAGAACAAAACAAATACACCACACATGCATACACCTGTCTCTTATACA10.5747126436781609No Hit
CCCCAGCCCTGCAGGGTCTCTGGCCACTCTTGTTTGTGCCTTCCACACCTT10.5747126436781609No Hit
GTCCATTAATCATCTCTGAAAAAAGTGGCATTGAATTTTAGCTCTATTTTA10.5747126436781609No Hit
TAGCAGTGTTCGCTCTAGTGAACTCAGAACCGCAGCTTCATTGCCAGAAGA10.5747126436781609No Hit
GCCCTGGCCAAGGAACCGCGAATCTTCAGTCTCTCAGAGACCACGGCTGGA10.5747126436781609No Hit
GTCATTATGCAGTCAAACTTGGAAGGTCTGTTGAGCATCTGTTGACAGAAA10.5747126436781609No Hit
GCCCAAGCAAGCCAAGGTAGTGGCCCGAAGGGAGAGCGGGGGCCGCCCCAT10.5747126436781609No Hit
GATCAGCAGCTTGCAGTGTGGACTGTACTTGACCTGTCTCTTATACACATC10.5747126436781609No Hit
TCTCGGAAAATGAGAAATACACACTTTAGGACATGAAATATGGCTGTCTCT10.5747126436781609No Hit
CCCCAGGACACAGAGACTTCATCAAAAACATGATTACAGGCACATCCCAGG10.5747126436781609No Hit
ACTTTACTCTTAGAAGGCATAAAACATTCATAGAATATATTTAAAAAATCA10.5747126436781609No Hit
AGCAGTCCCCAAGTTCATAAGCAAGGTCAAACTTGGGATTCTTTCTGTGCA10.5747126436781609No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers