FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_61.34100000007265.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_61.34100000007265.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258318
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC38851.5039602350591132No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT4260.1649130141918101No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT3980.15407366114633902No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA3290.12736239828428528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCCC4450.040.45553215
ACGAGAC4450.040.447722
CCACGAG4450.040.447720
TCGTATG4400.040.3959940
CCGAGCC4500.040.0060214
CCCACGA4500.039.9982819
TGCCGTC4500.039.9982845
CTCGTAT4450.039.94210439
GCGAATC4400.039.88464733
CGAATCT4400.039.88464734
GGCGAAT4400.039.88464732
GCCCACG4550.039.56639518
ATGCCGT4550.039.55873544
CGTATGC4600.039.12875441
TCTCGTA4550.039.0642538
CGAGACC4550.039.0642523
AGGCGAA4500.038.9983231
ATCTCGT4600.038.63964537
CAAGGCG4550.038.56976729
CTCCGAG4700.038.3036412