FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_60.34100000007259.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_60.34100000007259.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478177
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC41010.8576322156858234No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC207.0294715E-444.9995823
GCGAATC5500.039.68144633
TATGCCG5750.039.52137443
ACGAGAC5750.039.13007422
CGTATGC5700.039.07858341
TCTCGTA5650.039.02618838
AGGCGAA5600.038.9728531
AATCTCG5550.038.91855236
ATGCCGT5800.038.79274444
GTATGCC5750.038.73877342
GGCGAAT5650.038.6279632
CTCGTAT5750.038.34747339
ATCTCCG5900.038.1352410
TCCGAGC5900.038.1352413
CAAGGCG5850.038.0765729
CCGAGCC5950.037.81477414
TCGTATG5850.037.69195640
CACGAGA6000.037.4996521
TCTCCGA6000.037.4996511
AAGGCGA5850.037.30734630
Produced by FastQC (version 0.11.2)