FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_59.3410000000724c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_59.3410000000724c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106358
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC33473.1469188965569113No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5170.4860941349028752No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT4740.4456646420579552No Hit
CACTAACACTAACACTAACACTAACACTAACACTAACACTAACACTAACAC2910.27360424227608643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.12692980311777205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4350.035.68965541
TATGCCG4350.035.68965543
CCCACGA4350.035.68965519
CCACGAG4350.035.68965520
ATGCCGT4300.035.58139844
TGCCGTC4300.035.58139845
GTATGCC4450.035.39325742
GACCAAG4200.035.35714326
CAAGGCG4200.035.35714329
CGAGACC4200.035.35714323
GCCCACG4400.035.28409218
CTCCGAG4400.035.28409212
TCCGAGC4400.035.28409213
AGCCCAC4400.035.28409217
CACGAGA4400.035.28409221
CCGAGCC4400.035.28409214
CGAGCCC4400.035.28409215
GCGAATC4150.035.24096333
ATCTCGT4350.035.17241737
CTCGTAT4350.035.17241739
Produced by FastQC (version 0.11.2)