FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_58.3410000000723f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_58.3410000000723f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631251
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC17140.2715243223377072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2800.033.74875339
AATCTCG2900.033.36083636
GGCGAAT3000.032.2488132
CGTATGC3050.030.98246241
CACGAGA3100.030.48274421
ACGAGAC3100.030.48274422
ATCTCCG3150.029.9988910
GCGAATC3200.029.5301633
AGGCGAA3300.029.31709731
TCTCGTA3250.029.0758538
TCGTATG3450.028.69459240
TATGCCG3300.028.63530343
CGAATCT3300.028.63530334
CTCCGAG3400.028.45708512
CGAGACC3400.028.45483223
CGGCGCC803.3869583E-928.1261912
TGCGGCG803.3905962E-928.12396210
CCGAGCC3450.028.04466214
CGAGCCC3400.027.79529215
TCCGAGC3600.027.50116213
Produced by FastQC (version 0.11.2)