Basic Statistics
Measure | Value |
---|---|
Filename | H23NVBCXX l02n01 12h_57.34100000007222.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 76625 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC | 1699 | 2.2172920065252852 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT | 339 | 0.4424143556280587 | No Hit |
ATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTTC | 311 | 0.40587275693311586 | RNA PCR Primer, Index 46 (95% over 22bp) |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 258 | 0.3367047308319739 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 188 | 0.24535073409461663 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 142 | 0.18531810766721044 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 113 | 0.1474714518760196 | No Hit |
TATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTT | 93 | 0.12137030995106035 | RNA PCR Primer, Index 46 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACAGTGT | 25 | 0.0020948802 | 36.000004 | 31 |
CTCTTTA | 25 | 0.0020948802 | 36.000004 | 1 |
GAATATT | 25 | 0.0020948802 | 36.000004 | 4 |
TAAGGCG | 25 | 0.0020948802 | 36.000004 | 5 |
GTATCAA | 120 | 0.0 | 33.75 | 1 |
CGTCTTC | 45 | 3.4489334E-5 | 30.0 | 45 |
GTATAAA | 30 | 0.0051176697 | 29.999998 | 1 |
GGCGCAG | 30 | 0.0051176697 | 29.999998 | 13 |
TGTAAAG | 30 | 0.0051176697 | 29.999998 | 2 |
CCGAGCC | 240 | 0.0 | 28.124998 | 14 |
TATCAAC | 145 | 0.0 | 27.931034 | 2 |
GCGAATC | 235 | 0.0 | 27.765957 | 33 |
GAATCTC | 235 | 0.0 | 27.765957 | 35 |
CGAATCT | 235 | 0.0 | 27.765957 | 34 |
GGCGAAT | 235 | 0.0 | 27.765957 | 32 |
TCTCCGA | 245 | 0.0 | 27.55102 | 11 |
CGAGACC | 245 | 0.0 | 27.55102 | 23 |
ACGAGAC | 245 | 0.0 | 27.55102 | 22 |
ATCAACG | 140 | 0.0 | 27.32143 | 3 |
CGTATGC | 240 | 0.0 | 27.187498 | 41 |