FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_57.34100000007222.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_57.34100000007222.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76625
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC16992.2172920065252852No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT3390.4424143556280587No Hit
ATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTTC3110.40587275693311586RNA PCR Primer, Index 46 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.3367047308319739No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.24535073409461663No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.18531810766721044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.1474714518760196No Hit
TATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTT930.12137030995106035RNA PCR Primer, Index 46 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGT250.002094880236.00000431
CTCTTTA250.002094880236.0000041
GAATATT250.002094880236.0000044
TAAGGCG250.002094880236.0000045
GTATCAA1200.033.751
CGTCTTC453.4489334E-530.045
GTATAAA300.005117669729.9999981
GGCGCAG300.005117669729.99999813
TGTAAAG300.005117669729.9999982
CCGAGCC2400.028.12499814
TATCAAC1450.027.9310342
GCGAATC2350.027.76595733
GAATCTC2350.027.76595735
CGAATCT2350.027.76595734
GGCGAAT2350.027.76595732
TCTCCGA2450.027.5510211
CGAGACC2450.027.5510223
ACGAGAC2450.027.5510222
ATCAACG1400.027.321433
CGTATGC2400.027.18749841