FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_55.34100000007208.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_55.34100000007208.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391471
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC14510.37065325400859833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG1800.036.2496923
GACGATA1850.035.2699726
CACGAGA2000.033.74971421
CGATAAC1950.033.46125428
ACGAGAC2100.033.21422
AGACGAT2000.032.6247225
ACGATAA2000.032.6247227
CGTCCGT352.8171428E-432.14258622
CTCGTAT2050.031.82899739
CGTATGC2150.031.39508241
TATGCCG2150.031.39508243
TGCCGTC2150.031.39508245
GAGACGA2100.031.07116324
TCGTATG2200.030.68155940
TCTCGTA2150.030.3485838
TACGGCC300.005143375629.9997447
CCGTCGG300.005143375629.99974425
TCCGTCG300.005143375629.99974424
TATCTCG2100.029.99974336
ATGCCGT2250.029.99974344
Produced by FastQC (version 0.11.2)