FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_54.341000000071fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_54.341000000071fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC168.743169398907105No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC137.103825136612022No Hit
CCAAACACCTGACTGACGCTTACTTCAAGAAGAAGCAGCTGCGCAAGCCCA10.546448087431694No Hit
CTTATACACATCTCCGAGGCCCACGAGACAACATAATATCTCGTATGCCGT10.546448087431694No Hit
CAAGTAACCATGCCACATACTCTGAATACTACTTACTACCTTTGCACTGAA10.546448087431694No Hit
ACACCAAACTGACAGATTCCCAACTCTTCGGTTCAACCCAGAGGTCTTTTC10.546448087431694No Hit
TAGCCATGCTGTCCGCTCTCGCCCGTCCTGCCGGCGCCGCTCTCCGCCGCA10.546448087431694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGTGAAGA10.546448087431694No Hit
GTCCAAGGGTCCGCTGCAGCCGTGCAGGTCTTCGGACGCAAGAAAACAGCC10.546448087431694No Hit
GCATGGATCAGGTAAGAATCATATGGATAATGTTGAGTAGACAGCTGTCTC10.546448087431694No Hit
CATCTGGGGCATGCAGAATTCCTTCCACCAGTCGGACTCCTCTCATACACT10.546448087431694No Hit
GGGAGGGACGTGCTGTAGTTGATGGTACATGTGCTGTCTCTTATACACATC10.546448087431694No Hit
AAACAAAACATAACGAGAAACAAAAAAAAGTTCATACTGCGACCTACAACT10.546448087431694No Hit
GCTCCAGGAGGGCCTTCTAAATGGGAGGACAGACTGAAATGACTGTTCTTA10.546448087431694No Hit
ATGCTAGGTTATAATTTCAAACTGTGAAAACTGTCTCTTATAACATCTCCG10.546448087431694No Hit
GCGCGAGGACCTCGGCCCCGCGCGACTTCTACACAGCGGCGGCCTTCTCGT10.546448087431694No Hit
GCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTTC10.546448087431694No Hit
CAAGAAAACTGAAAATCATGGAAAATGAGAAATTTCCACTTGACGACTTGA10.546448087431694No Hit
GACATATACACCACACAATGAGCACACATCATATACATACTTACACAAAAC10.546448087431694No Hit
TTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC10.546448087431694No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG10.546448087431694No Hit
GTGTATACTCATTTTGCCTGAAGCTCTTTTACCTTTAATCGATAGACCTTT10.546448087431694No Hit
ATCTGATACCGTTATTCAATTTTGATATTGCATAGGGCTGTCTCTTATACA10.546448087431694No Hit
GTTTCCATGAGTTTTTAAGCTCTCTGCTGTTTCTGTTTTTTAAATCTAGAT10.546448087431694No Hit
CTACCTCTGGGCCTGCCACCACGCCCCTGGCTAGTGCACCAAGACCCTGTC10.546448087431694No Hit
GCCTCCTTGTTTGCCTTGGTATGAAGAGCTGCCTCCGGAGCCTGTGCCATA10.546448087431694No Hit
GAGCTATAGGCTCTTCTCAGTCCTATAGACAAACTGTTTTATACCTGTCTC10.546448087431694No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT10.546448087431694RNA PCR Primer, Index 44 (95% over 22bp)
CAGCTAGAGGGGTCCTGGTTGAAAGAAGGCACATAGTCTGTGTGGGACACC10.546448087431694No Hit
GCTTTAGAGAGATGGTCAGTGGCTAAGAGTACTTGTTTTGCGAGAGGACAT10.546448087431694No Hit
CAAACAGGGTTGGGGGGAGGGCATGCAAAAACTAACATTGAAATTTTGAAA10.546448087431694No Hit
GGGTACTAGCATTCAGTACATTTCCTCCTAGTTGGCTACCTGTGTTTCCCT10.546448087431694No Hit
AGTCACAAGTGGGCTACCTAGCAAAGCCTAATAAGCTTTGAGATACTGGGC10.546448087431694No Hit
CTTTTATTTGAGGGACAGACAAGTACTACATTCTTTCCACATAGACAGCCC10.546448087431694No Hit
CTTATACACATCTCCTAGCCCACGAGACAACATAATATCTCGTATGCCGTC10.546448087431694No Hit
AATTTTGACAGTGTAAAAACTGTATCAACCTATCAAATAATGCTTGGAGAA10.546448087431694No Hit
ATCCTGTAATTTGTCCTGACAACACAACTGGTTCACCGCTTGCCTTCTGCT10.546448087431694No Hit
GAACAAATGAAAGCAGAGGTAATGAAACAGAGAAGCAATAAAATGGGGAAA10.546448087431694No Hit
GGACTTCTAGAAGTCTGAGGGCTGCTTTGTTTAAAAGCAGAGCCTCGAGGT10.546448087431694No Hit
CTGCACAGGTGCACAGGTGAAAGCAGAGCCAGGCAATACTGCCTAAGACAG10.546448087431694No Hit
CAATAACTCTTGAAGTCCTATTAAAGCGTTGGAATGGAACAAACATGATTG10.546448087431694No Hit
GGATTGCGCAGTAAACATTCCCTGTGTGGTCTGAGTCTCAGACTCATCTGC10.546448087431694No Hit
ATACTAATAGAATCAAAGACCTCCAGTGATGCCAACCATATTTCAAGTAGT10.546448087431694No Hit
GAGTTGTTCAATCCATGGGATTCACACGATGCAGGAGCTGTCTCTTATACA10.546448087431694No Hit
AACTACTGACGTAGTGGTCTACTGTATATAACATCTAATACATAGTACAGC10.546448087431694No Hit
CTACACATCCCAGCATGCTTCGCCGGCCGCGGGGCAGCGAGGCGCGGTGAG10.546448087431694No Hit
AATATACCGTCGGCCATATGAAAAGGGACGTCTAATCATTTAGTTTAAGGT10.546448087431694No Hit
ATTTTCCATTTTTCTCATTGTAACGCATTGATATACACTGTTCTACCATGC10.546448087431694No Hit
GACAGAGACTGATAAATCACACTCTATGAGAAAATGCGGTGTAGAAACCTG10.546448087431694No Hit
GTTATATCAGCAAACCAACTGTAAGTCATTTTTAAAACAAATTATTCCCCA10.546448087431694No Hit
CGTGTGATCAGTCTCCTTCATGTTTAACTCCGATCCACACCAAACGTGCAT10.546448087431694No Hit
CAGTTGTCTAATGGGATCTATATGAAGTTAGCTATGTCTGTATGCCCTTCT10.546448087431694No Hit
ACTTTAAGGTGTATGCTTCCTCAAATGGTGATTATGTGAGATCTGTCTCTT10.546448087431694No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCAT10.546448087431694No Hit
ACCCAGAGAAGCACACTTTGTGAGGACTAATGGAAAAGCTGTCTCTTATAC10.546448087431694No Hit
TTTTTTTTCCTGTGTCCATACCCTTGCAGCGGCGCTCAAGCGGGCGGCTGC10.546448087431694No Hit
CCTGACTGGTGTTACCTCCTTGTAATCTGCAGACTGAGCTTGTAATAGGAA10.546448087431694No Hit
GAACTACTCCATCAATAGGCAAAGTGTAACCAAAATCTGTCTAGATGGATA10.546448087431694No Hit
GCCCAAAGGAGAAGGGGGAGATGTTCAGCATGTTCAGCAGTGTGGCCTCTG10.546448087431694No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGA10.546448087431694No Hit
GAATAACATGATTTTCTAGAATTAGCAGACGGATACATGACCTTGGTGCAA10.546448087431694No Hit
CCTCTGAGCTTCTCTATCGCTTTCTCTCTCTCTGCTCCGGGACCTCGACTT10.546448087431694No Hit
GTCCAGCTGCCAGAGCAGCTGGCTCTCAATGGGCAGGCTGTCTCTTATACA10.546448087431694No Hit
CTCTTACCCAATCAGTTCTTCAACCCCAAATTTGTCTACACAACTACCTAT10.546448087431694No Hit
ATTGACATGGCACTGGTAAATAAAACTGTATGCAAAGCAATGGTTATTATC10.546448087431694No Hit
CCTCTACAGTGCTCCACAGGACTCTTGTCCCTTCCAGTGAATCCAGTGAGT10.546448087431694No Hit
GAATCATGTCTTAACGTCTCCGTACTGAGTTGTGGGCACAGCTTGCTGCTG10.546448087431694No Hit
GTGCAGTCCAGAGCATCCCTGCTTCTTGGCAATGGCGTGTGAGCTAAAGTG10.546448087431694No Hit
TCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCTTTGGTCTCCCTG10.546448087431694No Hit
TACGACCACCACGGCGGACACGAATCCTGTAAATGACATAGCCCTGTCTCT10.546448087431694No Hit
CCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCGGATCATCCTCT10.546448087431694No Hit
ATACTACAAAGTTACTTTTCTGGCTCATTGAACCATAACCCAAATACTGAA10.546448087431694No Hit
ATACTGGAGTATTCATACATGACATTTTAAGTGACCCAGTAGAGAGCAAAA10.546448087431694No Hit
GATCCATGCAGAAGAAAAGCTCAGGCTGGCAAATGAGGAGATCGCCCAGGT10.546448087431694No Hit
AACCCTGAGCATAAAACATGCACAATAAGAGAAGAGAGTATATGTTGGACA10.546448087431694No Hit
CTCTTTTTCCGTGATTTTCAGTTTTCTCGCCATATTCCAGGTCCTACAGTC10.546448087431694No Hit
GGGCACAGGATAAGTGTTATTAGTCAATTGCAGGAGATCCTTAGAACAAGA10.546448087431694No Hit
TTTCCTGCTTCTTCTTGGTGTCACTCATCTTATGGTTTGTATCTTCACTCT10.546448087431694No Hit
ACACCAAGGTCTTGCCCTCAAATTCCTTCAGCTGTTGCACACAATACTCAT10.546448087431694No Hit
TAGTTATAGCAGTTACAACAAACCAAAGTGACTGAGTTTGTACAAATGGTA10.546448087431694No Hit
CCCACAACCTCTGTGAGCTCATGTGTGCAACCTGGCTTGCCTTGACTATGA10.546448087431694No Hit
CAGAAAAGTTGCAGATGGAGCAACAGCAGCAACTGCAGCAACCTGTCTCTT10.546448087431694No Hit
TCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCCTGC10.546448087431694No Hit
ATATACCGCCATCTTCAGCAAACCCTAAAAAGGTATTAAAGTAAGCCTGTC10.546448087431694No Hit
TTCTGAGAGAGGGTGTTATCAGTTTGTGGAATGGCTTCCTGGTTGCAGAAA10.546448087431694No Hit
ATCATAAACTTAACTTTGCAATCCAGCTAGACTTGAAGGGGAATGAGGAAA10.546448087431694No Hit
TTTCTATGCCAGCCAGATGGCTCAGCGGAAGATCCGAGACATCCTGGCTCA10.546448087431694No Hit
GAAAAGTATGCTACTTAAGGAGTCTTTCTTCTGGATTTAAAAATGCAGGTT10.546448087431694No Hit
GCAGTAGTGTGTGTCTGCCACATATGCAGACTCACAAGAACCTACTACACA10.546448087431694No Hit
GGTCCTGCACTGGTACAGCCTTGTGTATTGTCCCCATACTGATGGACGTGG10.546448087431694No Hit
CTTCTAGAGTATTTTAAGAGTTTCATTGAAACCCTGTCTCTTATACACATC10.546448087431694No Hit
GTCTTCAATAGGAACAGATTTTGCTTCATGTATCCAGCTCCTCTGCAGGGT10.546448087431694No Hit
GGAAAGAAAAGAAAAGAAAAGAAAAGTAGTTACAGGATGTTGCAAGTCCCC10.546448087431694No Hit
ACAGGGACCCCAGCCTGAGGGGTTGTCCTGGATCACAGGTGTCATGAACTT10.546448087431694No Hit
ATACAATGTCTCGAGTATGCTGTGTAGCTTCATTTCTTTAGAAATGAAAGC10.546448087431694No Hit
CATGGTACTTCACTACCATCTGTAACTCAAGTTCCAGGAAATTCAATGCCT10.546448087431694No Hit
GAGTGAGATCACACGATCTTCAATGGACACATTGGCCACACCGTCCTTTCC10.546448087431694No Hit
CTTATACACATCTCCGACCACGAGACTTGCTGGAATCTCGTATGCCGTCTT10.546448087431694TruSeq Adapter, Index 8 (95% over 22bp)
GTAAGAATATCAAGAACTGTGGCTTTTGAGACAAGGTTTCTCTGTGGCCTG10.546448087431694No Hit
TTATACACATCTACGACCCACGAGACTTGCTGGAATCTCGTATGCCGTCTT10.546448087431694TruSeq Adapter, Index 8 (95% over 22bp)
CGCCCACCCTGATCGCCGACCTCTTCGTGGCAGACCAGCGGAGTCGGATGC10.546448087431694No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGAC10.546448087431694No Hit
GCCTCCAACTTGTCTCGGGAGATTGAGTCTGCACCTGTCTCTTATACACAA10.546448087431694No Hit
CACCTAAAGAGGCCATTGAGGGTACCTACATCGACAAGAAATGCCCCTTCA10.546448087431694No Hit
TACCATCTTCTGAGTCTTCTTGCTGTTCATAATCTTTCAGAAATTGAATTT10.546448087431694No Hit
ATTCAGCACCAGACCTGGCTGGACAGCTCCCGGTTTCTCTGAGGCTCAAAA10.546448087431694No Hit
TTCCATTACCCAACACAGCTCACTGCTGTCTCCACCTTACACCCATGGGGT10.546448087431694No Hit
CTCTTCAGTCGCTCATTGAGAGCCTTTAGGGCTAGTTGCCTTCTGCGCTCC10.546448087431694No Hit
CCTCATGAGAGAGTATACTTTTTGAGCCTAACTGCTATGTTTTCTGCAGAA10.546448087431694No Hit
TGCCAGGATTGCTGTAAGACAATTCCAAAATGTTAGAAGTCAAGCAAGTGA10.546448087431694No Hit
GACAGGGGACAGGTCAGGAACAGGTGAAGCTCTTACCACCCTCTCAGAGGT10.546448087431694No Hit
GAGCAGAAGGCCGCCCCGTGCGTGCTGGACACACAGCTACTTCTGCCTTTC10.546448087431694No Hit
CCAAGTGGCCAGGTACCGCCCTCGGGCTCCTATCATTGCCGTGACTCGAAA10.546448087431694No Hit
GCCCAGGTCCTCTTGTGGGGAGCACAGGGATTTCTTCTCCCATAGATATTT10.546448087431694No Hit
GGTATGAGGAATCACAACTATCAATCATAGCTTCTTACAAGCTGTCTCTTA10.546448087431694No Hit
CTTGAGCTACAGAGGGAGACCCTGTCGCAAAAAAACAAACAAACAAACAAA10.546448087431694No Hit
GAAAGAACATGGCTACCGTGAGACAGCCCTGTGGGCCCTGCTACCCCTCCT10.546448087431694No Hit
CAAAGGAGCCGATGACGCAGCAGACGCAGACACAGCTATAATCAATGCAGA10.546448087431694No Hit
GATTGGAAGTCATAAATTCTCCAAGTTATTCCAAGGTAAGTCAGCATCTGT10.546448087431694No Hit
GCCAGGGACCATATCAACAATGGCAGCATCGCCAGACTTCAGGAACTTGGG10.546448087431694No Hit
GTCTCTGGTTTTATGTGGAGTACTTTGATCCACTTAGACTTGAGTCTTGTT10.546448087431694No Hit
CTTATACACATCTCCGAGCCCACCAGACTTGCTGGAATCTCGTATGCCGTC10.546448087431694No Hit
GAGTGAGGCTCCACCCTTTCCATGTCATCCGTATCAACAAGATGTTGTCCT10.546448087431694No Hit
GTCCAGTATTACCCTTGGCATTATCTTAGCCTTCACTTTATCCACAGTCTT10.546448087431694No Hit
ATCCGCAACAGAGCGTGGTGATGAGAGTGGCCAACCTCTGTCTCTTATACA10.546448087431694No Hit
GTTAATCAACTATTACATGCCAACAGCTATAAAGAAAAACAGAGCAAACTT10.546448087431694No Hit
ACATCACTATGACCTGTGGTTCCCCATGCAAGTGGCTACCACTCCCACGAG10.546448087431694No Hit
CAGTCACACCTTTCCATCTATGCCAACGTCGGCAAAACTATCACATAATGC10.546448087431694No Hit
CTTATACACTCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT10.546448087431694Illumina PCR Primer Index 8 (95% over 21bp)
ATGCCAACCCTCCATTTTTCCTCTCCAGATATTTTTGGGAGTGACAAACAT10.546448087431694No Hit
CCCCATGACCATGGGGTGACTGGAATGTGGCTTTGATGGCTCAGGCTGCTG10.546448087431694No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT10.546448087431694No Hit
CCTTTACATCTTTCTTTGAGTTTATATTAGCTAAAAGGATTGATGGAAAAT10.546448087431694No Hit
AGCAAAGAGAACTGCACAATCTCTTCTCCAAGTCTAAGTTAAACAGAGCCG10.546448087431694No Hit
GCCCCAGCACAGGACAACATCTTGTTGATACGGATCACATGGAACTGTCTC10.546448087431694No Hit
CTGGTGTGTGTGTGTGATACGAATGCATAGAAGAGCGAGAACCTGTCTCTT10.546448087431694No Hit
CAGTCATGCCTTGTTCAGTGCCAGTCAGAACGTAACTACCGGTCCCCATGG10.546448087431694No Hit
CCTGTCTTGGGATACCCCTACTCTGGGGCATCGAGCCTTCACAAGACAAAG10.546448087431694No Hit
TAAGGTGCATCAGTAATTCCTACAAACTTAGCACTTTAGCCAGGATATTAT10.546448087431694No Hit
CCTCAGAAGACCCCCGTCAGAGGATGAAGGAATGGATATCCACTTCGAGGA10.546448087431694No Hit
GTATATATGTTCCGAAGAAAAAATGTATCTCTACATCCTGTCTCTTATACA10.546448087431694No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCACCATAT10.546448087431694No Hit
GTATAGAACAGAAAACCTGTCACAACAATCACAGGTATCTTTTCAAACATC10.546448087431694No Hit
GATTATGAGATGAACCCGAACCATAAAGATGGTAAGAAGGAAGACTCCGTG10.546448087431694No Hit
GAAATATGGCGAGGAAAACTTAAAAAGGTGGAATATTTAGAAATGTCCACT10.546448087431694No Hit
GGACCTAGACACCATGCAGAGGCTCAACATCGGCTATGTCTGTCTCTTATA10.546448087431694No Hit
GTCTCAGACACCTTGGAGCTTGTCTTCCCCTGGGGCTAAAATGAGACTGTG10.546448087431694No Hit
TGCCAGCCCAGGCTACTATACCCAGCCAAACTTTCAATTACCATAAATGGA10.546448087431694No Hit
TTTCTACATTCCTTTTTCTGAACCTTCTTGGAGGTTTCCTTTTCAGAAGCA10.546448087431694No Hit
ATACAGTGGTATTTTTACAGAGTGTTGTACATGTCCCCTTTGGAAAATGGA10.546448087431694No Hit
GTTCACCACCTGCTTGCGGACCCTAATGTGACGTTGGCGGATGAGCACACG10.546448087431694No Hit
CACACACACACACACACACACACACACACGCAGTGCTGTCTCTTATTCACA10.546448087431694No Hit
GCTCAGAGGCCACAGACCCCAGGTTCTCACCTCCTTCATTGGAAACAAAGC10.546448087431694No Hit
CTATTAAAGCGACAGCAGAAGCTGGAGCAGCTTGAACTCTGTCTCTTATAC10.546448087431694No Hit
AGTATCACCGGGTGGGTTTCTCAGCCCCTCCTGCCCCACCCTACAGAGATC10.546448087431694No Hit
CCTTATACACACTGTAACGTGCAGCCAGGCTATGAGCTACTGTCTACCTGC10.546448087431694No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers