FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_53.341000000071ed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_53.341000000071ed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291305
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC18390.6312970941109833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG2500.039.5984936
TGCCGTC2500.039.5984945
CCGAGCC2600.038.9475114
CACGAGA2600.038.94082321
CCCACGA2600.038.94082319
CGATAAC2500.038.69852428
CGAGACG2650.038.2060923
CCACGAG2650.038.2060920
AGACGAT2550.037.93972825
GACGATA2550.037.93972826
CGAGCCC2700.037.50500515
TATGCCG2700.037.4985743
TCTCGTA2700.037.4985738
ACGATAA2600.037.2101227
GCCCACG2750.036.81677618
GAGACGA2750.036.81677624
ACGAGAC2750.036.81677622
ATCTCGT2700.036.66526837
CGTATGC2800.036.15933641
CGTTCTA250.002104926635.99862724
Produced by FastQC (version 0.11.2)