FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_52.341000000071d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_52.341000000071d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332219
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC15240.4587335462450974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGATA1950.036.9229526
CGATAAC2000.035.99987828
CTCGTAT2200.035.79533839
TATGCCG2200.035.79533843
AGACGAT2050.035.12183425
ATCTCCG2250.034.9998810
ACGAGAC2250.034.9998822
ACGATAA2100.034.285627
CGAGACG2100.034.285623
CGTATGC2350.033.5105341
CACGAGA2350.033.5105321
CCCACGA2350.033.5105319
TATCTCG2350.033.5105336
ATGCCGT2350.033.5105344
GAGACGA2150.033.48826224
GCCCACG2400.032.81239318
TCCGAGC2450.032.14275413
TCGTATG2450.032.14275440
CCGAGCC2550.031.76459914
CGAGCCC2500.031.49989715
Produced by FastQC (version 0.11.2)