FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_51.341000000071c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_51.341000000071c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338813
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC18390.5427772842246312No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGCG207.0265774E-444.99955721
GATCGGC250.002105118235.99964534
CGAGACG2550.034.41142723
TGCCGTC2550.034.41142745
CGTATGC2750.033.54512441
TATCTCG2750.033.54512436
GACGATA2500.033.2996726
TATGCCG2750.032.7269543
ATGCCGT2700.032.4996844
GCCCACG2850.032.36810318
CCCACGA2850.032.36810319
TCTCGTA2850.032.36810338
TCGTATG2850.032.36810340
ACGAGAC2850.032.36810322
AGACGAT2600.032.01891725
CGATAAC2600.032.01891728
ATCTCGT2900.031.81003237
CTCGTAT2900.031.81003239
CACGAGA2900.031.81003221
CCACGAG2900.031.81003220
Produced by FastQC (version 0.11.2)