FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_50.341000000071b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_50.341000000071b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239617
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC11140.46490858328081897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1800.042.4992139
TCTCGTA1800.042.4992138
ATCTCGT1850.041.35058637
CGAGACG1850.041.35058623
CGATAAC1850.041.35058628
TATGCCG1900.040.2624143
TATCTCG1900.040.2624136
TCCGAGC2000.039.37426813
CGTATGC1950.039.2300441
AGACGAT1950.039.2300425
ACGATAA1950.039.2300427
ATGCCGT1950.039.2300444
GACGATA2000.038.2492926
ACGAGAC2000.038.2492922
CCACGAG2000.038.2492920
CCGAGCC2100.037.49930614
CCCACGA2050.037.31638319
GAGACGA2050.037.31638324
GTATGCC2050.037.31638342
TCGTATG2050.037.31638340
Produced by FastQC (version 0.11.2)