FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_5.34100000006ea0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_5.34100000006ea0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486704
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC6830.14033170058187316RNA PCR Primer, Index 27 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAG301.13796006E-437.50556612
CGAGATT450.001224478225.0037115
CGAGACG1101.5097612E-1024.54405423
TAGGACG851.890021E-723.822174
GACGAAT952.1080268E-823.68529126
CTCGTAT952.1103915E-823.6828639
TATCTCG952.1103915E-823.6828636
ACGAGAT602.462199E-422.50333814
TATGCCG1104.0399755E-922.49871643
CGTATGC1257.4942363E-1021.59876841
ATGCCGT1156.7484507E-921.5205144
ACGAGAC1156.7484507E-921.5205122
CGAATTG1056.086702E-821.4295528
GGTATCA1501.4551915E-1121.0052761
GAGACGA1301.2223609E-920.7701824
ATCTCCG1201.0993972E-820.62806110
CACGAGA1201.1019438E-820.62382321
TCTCGTA1109.960422E-820.45337938
TCTCCGA1251.7580533E-819.8029411
GTATCAA2800.019.2905581
Produced by FastQC (version 0.11.2)