FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_49.341000000071aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_49.341000000071aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361642
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC14960.41366876634904126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAC1750.037.28468328
ACGATAA1850.035.26929527
CACGAGA1950.034.61442621
AACCTAT1900.034.34115632
GACGATA1900.034.34115626
TCTCGTA2050.034.0234538
CTCGTAT2000.033.7490739
TATGCCG1950.033.46061343
CCGAGCC2100.033.2179614
TCGTATG2100.033.21336740
ACGAGAC2100.033.21336722
GAGACGA2050.032.9259224
TATCTCG2050.032.9259236
CGAGACG2150.032.44096423
ATCTCCG2100.032.1419710
CGTATGC2100.032.1419741
AGACGAT2100.032.1419725
TAACCTA2050.031.82838831
TGCCGTC2050.031.82838845
ACCTATC2150.030.34799633
Produced by FastQC (version 0.11.2)