Basic Statistics
Measure | Value |
---|---|
Filename | H23NVBCXX l02n01 12h_48.3410000000719d.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 42041 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC | 2127 | 5.059346828096382 | RNA PCR Primer, Index 46 (95% over 21bp) |
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT | 244 | 0.5803858138483861 | RNA PCR Primer, Index 18 (95% over 21bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 160 | 0.3805808615399253 | No Hit |
ATATGAACTCTCGCAAACGATAAACCTGTTATCCCTAGCGTAACTTTTATT | 102 | 0.2426202992317024 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 71 | 0.16888275730834185 | No Hit |
CATATGTTCTATCCTTGAGAATGATCCATGTGCTGAGGAGAAGAATGTGTA | 68 | 0.16174686615446826 | No Hit |
GTATAGATTCTATCCTCGGAAGGAAACAAGAATAGAAGAAGAACCTCTAAT | 57 | 0.13558193192359838 | No Hit |
GTACATAACGAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACCAATGAG | 55 | 0.13082467115434931 | No Hit |
GTGTATATGTGCCACATTTTCTTAATCCAGTCTATCATTGTTGGACATTTG | 51 | 0.1213101496158512 | No Hit |
CTATTATTGGAGCATATCAGCAGGAAAAACAGTTCTACTGAAAAGCAAAAA | 45 | 0.107038367308104 | No Hit |
ATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTTC | 45 | 0.107038367308104 | RNA PCR Primer, Index 46 (95% over 24bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTTATAC | 275 | 0.0 | 40.090908 | 1 |
GTATGCC | 280 | 0.0 | 39.375 | 42 |
TATGCCG | 280 | 0.0 | 39.375 | 43 |
CATTCCG | 280 | 0.0 | 39.375 | 29 |
CGTATGC | 280 | 0.0 | 39.375 | 41 |
TCGTATG | 280 | 0.0 | 39.375 | 40 |
ATTCCGA | 280 | 0.0 | 39.375 | 30 |
GACCATT | 280 | 0.0 | 39.375 | 26 |
CTCGTAT | 280 | 0.0 | 39.375 | 39 |
TGCCGTC | 275 | 0.0 | 39.272728 | 45 |
ATGCCGT | 275 | 0.0 | 39.272728 | 44 |
GCCCACG | 290 | 0.0 | 38.793102 | 18 |
CCCACGA | 290 | 0.0 | 38.793102 | 19 |
ATCTCCG | 285 | 0.0 | 38.68421 | 10 |
TTCCGAA | 285 | 0.0 | 38.68421 | 31 |
TCTCGTA | 285 | 0.0 | 38.68421 | 38 |
TCTCCGA | 285 | 0.0 | 38.68421 | 11 |
AGACCAT | 285 | 0.0 | 38.68421 | 25 |
CCGAATC | 285 | 0.0 | 38.68421 | 33 |
AGCCCAC | 290 | 0.0 | 38.01724 | 17 |