FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_48.3410000000719d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_48.3410000000719d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42041
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC21275.059346828096382RNA PCR Primer, Index 46 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT2440.5803858138483861RNA PCR Primer, Index 18 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.3805808615399253No Hit
ATATGAACTCTCGCAAACGATAAACCTGTTATCCCTAGCGTAACTTTTATT1020.2426202992317024No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.16888275730834185No Hit
CATATGTTCTATCCTTGAGAATGATCCATGTGCTGAGGAGAAGAATGTGTA680.16174686615446826No Hit
GTATAGATTCTATCCTCGGAAGGAAACAAGAATAGAAGAAGAACCTCTAAT570.13558193192359838No Hit
GTACATAACGAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACCAATGAG550.13082467115434931No Hit
GTGTATATGTGCCACATTTTCTTAATCCAGTCTATCATTGTTGGACATTTG510.1213101496158512No Hit
CTATTATTGGAGCATATCAGCAGGAAAAACAGTTCTACTGAAAAGCAAAAA450.107038367308104No Hit
ATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTTC450.107038367308104RNA PCR Primer, Index 46 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC2750.040.0909081
GTATGCC2800.039.37542
TATGCCG2800.039.37543
CATTCCG2800.039.37529
CGTATGC2800.039.37541
TCGTATG2800.039.37540
ATTCCGA2800.039.37530
GACCATT2800.039.37526
CTCGTAT2800.039.37539
TGCCGTC2750.039.27272845
ATGCCGT2750.039.27272844
GCCCACG2900.038.79310218
CCCACGA2900.038.79310219
ATCTCCG2850.038.6842110
TTCCGAA2850.038.6842131
TCTCGTA2850.038.6842138
TCTCCGA2850.038.6842111
AGACCAT2850.038.6842125
CCGAATC2850.038.6842133
AGCCCAC2900.038.0172417