Basic Statistics
Measure | Value |
---|---|
Filename | H23NVBCXX l02n01 12h_47.34100000007180.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 387504 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC | 8154 | 2.104236343366778 | RNA PCR Primer, Index 46 (95% over 21bp) |
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT | 854 | 0.22038482183409722 | RNA PCR Primer, Index 18 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 1055 | 0.0 | 38.385986 | 45 |
ATTCCGA | 1055 | 0.0 | 38.17273 | 30 |
CCGAATC | 1050 | 0.0 | 38.140236 | 33 |
CATTCCG | 1070 | 0.0 | 37.847862 | 29 |
TCCGAAT | 1065 | 0.0 | 37.8143 | 32 |
TCTCGTA | 1065 | 0.0 | 37.8143 | 38 |
CGAATCT | 1065 | 0.0 | 37.8143 | 34 |
GCCCACG | 1085 | 0.0 | 37.53198 | 18 |
CCCACGA | 1085 | 0.0 | 37.53198 | 19 |
TATGCCG | 1080 | 0.0 | 37.49742 | 43 |
CTCGTAT | 1075 | 0.0 | 37.462536 | 39 |
TCTCCGA | 1090 | 0.0 | 37.37428 | 11 |
CCGAGCC | 1090 | 0.0 | 37.369457 | 14 |
AATCTCG | 1080 | 0.0 | 37.2891 | 36 |
CGAGCCC | 1095 | 0.0 | 37.198822 | 15 |
CGTATGC | 1090 | 0.0 | 37.153404 | 41 |
TCGTATG | 1090 | 0.0 | 37.153404 | 40 |
TTCCGAA | 1085 | 0.0 | 37.11726 | 31 |
TCCGAGC | 1100 | 0.0 | 37.029736 | 13 |
ACGAGAC | 1100 | 0.0 | 37.02018 | 22 |