FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_47.34100000007180.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_47.34100000007180.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387504
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC81542.104236343366778RNA PCR Primer, Index 46 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT8540.22038482183409722RNA PCR Primer, Index 18 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10550.038.38598645
ATTCCGA10550.038.1727330
CCGAATC10500.038.14023633
CATTCCG10700.037.84786229
TCCGAAT10650.037.814332
TCTCGTA10650.037.814338
CGAATCT10650.037.814334
GCCCACG10850.037.5319818
CCCACGA10850.037.5319819
TATGCCG10800.037.4974243
CTCGTAT10750.037.46253639
TCTCCGA10900.037.3742811
CCGAGCC10900.037.36945714
AATCTCG10800.037.289136
CGAGCCC10950.037.19882215
CGTATGC10900.037.15340441
TCGTATG10900.037.15340440
TTCCGAA10850.037.1172631
TCCGAGC11000.037.02973613
ACGAGAC11000.037.0201822