FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_46.34100000007173.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_46.34100000007173.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC2110.5No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC42.0No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21.0No Hit
CTTATACACATCTCCGAGCCACGAGACTTGCTGGAATCTCGTATGCCGTCT21.0RNA PCR Primer, Index 8 (95% over 21bp)
GTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGACAGATCCTCTTCAGCTG10.5No Hit
GCTGGACAGTGGCTTGCTGCTTGTGACTGGGCCTCTTGTCATCAACAGAGT10.5No Hit
TACCAGCGATGAGCCTCCCATGCAAGCCAGCCAGAGCCAACTGCCCAGCAG10.5No Hit
AGATACATCTTATTAACATTTGTAGCTTCTAATAATAATAATAACAATAAC10.5No Hit
CTTAGGGGGAGTCTCTTTTGGTCCTCTGCCCTCTCCCTGTCTCTTATACAC10.5No Hit
CAGTGAGACCCTGTCTCAAGAAGCGAACAAAAGCCGGGGCGTGGTGGCGCA10.5No Hit
TTTCAAGGGTATGCCTTGATCTTACATCGGAAAGTGGATCTCTTGCTCTAA10.5No Hit
CCATAATCTTCAGTTTTCTTGCCATAATCCTTGTCCTACAGTGGACATTTC10.5No Hit
CCAATAAATGCAGGAAGGGTACTCAAGACTAAAGGGGGCGCGATGGCATGG10.5No Hit
GTTATAACTAGTTTGAGTTATTTTCTAGCCATACTTTCAAGTCTGTCTCTT10.5No Hit
CTACCACAATATGTTGAACAGACTCTTCTTCCTTCAGTAGACTTGAAACCC10.5No Hit
GGTATATATTGCCTGTTGGATTTTGGACAGAAGATTCAATGAATGGCACCT10.5No Hit
TCACTGTTTAGGATGAACACCTGCATGCTTTCCTGAGAACTACAAGCCCAT10.5No Hit
GCTTAGGGCTTTTCTTCTCTTATCCCTAGGCCCGGCTGGACATTTTGAAAA10.5No Hit
GATATCAACGGCATGCAACACCACATGTGGCTCATGAAAGATATTCATTTA10.5No Hit
GAGCCTTCCTTGTGGGCGTGTACGGCCAGATTGACACCAGCGCCCAGATAT10.5No Hit
TTTTTTTGCTGTCCCAATGATGCATAGTTGAGGATGTGGAGACTGTCTCTT10.5No Hit
GATCTAGGTCAAATGAAAGGAAATAGAAGACCAGTTTGCAAAAGTGGTGTA10.5No Hit
ACCTGGTGGTTCATGCTGTAGCCATTTTTGTATTTTGTGTGTTCCCTGTCT10.5No Hit
CCAGTCAGCAACAGCCCCATCCCTCAATGCCAGTTTCCCTCATTACTTGTG10.5No Hit
TTTCGAGACAGGCTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTTT10.5No Hit
AACCAAGACACTGTCCAGGCCCTTCCAGCCCGCCAAATACACGAGCTCTTC10.5No Hit
CTTCCAGGCGATGCGGTGGGGTCCCGGCGGGTCATGCTCCAAGAACCCATA10.5No Hit
TGATAGAGGGTAAGGATCTTAGAAATGATAGGAAGTACAACTGTCTCTTAT10.5No Hit
AATTTGTTCATTGCATCTGAAACTGAATTTCAAGAGTTATGTATGGTCTGT10.5No Hit
CTTCCTAATTTGTGTGCACTCGTGCGTGTGTGATTGTGTGCATGGTCCCCT10.5No Hit
GGAAAGGACGGTGTGGCCAATGTGTCCATTGAAGATCGTGTGATCTCACTC10.5No Hit
CAGCCAAAGGATCCTCTAGTCACAGGTTCCTGGGCTGGTGCAACTCTGAGC10.5No Hit
CTGTAGAATTTTGTTTACAAATACTACTAAAAAGGTCATGATCAGGAGTGC10.5No Hit
GAAGAAAGATGAGAAGAAGGAGGAGTCCGAGGAGTCGGATGACGACATGGG10.5No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATTTCGTATGCCGTC10.5No Hit
ATATAAACCAGGTGGGCCTTGAACTCACAAAGATCTGCCTGCTTCTTCCTT10.5No Hit
ATCCTTTCCTGTGTTGGGTTTGGGCTGTTCTTGCTCAGAATTCTGTAAGGT10.5No Hit
GACTAACATCACTGCTAAAAGTCCTCTAAATACCCCATGACAATCTCTATT10.5No Hit
ATTTAACAGTTGCAGATATCCACTACTTAGCCCACGGACTGTCTCTTATAC10.5No Hit
GGTCACATACATCCAGTCTGAAGAGAACAAAGAATGTTAGTGCTAAGCCAG10.5No Hit
GTACATGCACCCAGCCACTTGCATGCCTATTTTTCTCACAGAGACTGGGAG10.5No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT10.5RNA PCR Primer, Index 44 (95% over 22bp)
GAGAAAGAGATGGAAAACAGCCCCAAGGAAGGAGGCCTAATAGTATTGAGA10.5No Hit
AGCATGAACTGTGTGTGAGGTAGGGGGAGAGCGGGAACGGAGCGGAGGGAA10.5No Hit
ACGTTGGGCACTTCTAAACATCTAGATAGACCAGATGTTTCAAGTAAGGAC10.5No Hit
TTTCTCTCCTGGTGTCTTTTAACAAAATGAAGAAGCTGACAACTGATGGGA10.5No Hit
AATCAAAACCCACTACTTCAGAATCAATAGCTTCTTTGAAGCCACAGTAAC10.5No Hit
GTGTTCTAGCCATGCAGTTACATCTCTTTCTAAATGAGGTCAGGTTTGCAA10.5No Hit
GTACAGAGAAACAAAACATTAAAATACTGTCTGTCTCTTATACACTCTCCG10.5No Hit
CTTCCAAGTCGCTTTGGTTTTAAGAATTTCCTGATGCTCTTACAGTCTGCC10.5No Hit
CTTCCTAGAGATCTAGAATCTTGTCGCTGGCTTTTCTTCTTTTCTCTCTTC10.5No Hit
GTCCCCATACTGATGGACGTGGAACCCATGCTGGCCTTCAGTTAATCCTGT10.5No Hit
CATATATGTCAGTGTCAGATTAGACACTGATACTTTTGTTCCTGTCTCTTA10.5No Hit
CATCAGCAAGGTAACAGTTCATACTTATTGCCTAAGCATATTGACCTTAAA10.5No Hit
CTCCAGGAGCATCCTGCTATTTAAGAAAAACAAATTCTCTTCTTTTGTCAG10.5No Hit
TTGTAAGAACCAATGATACTTGCTGAGGTGATCTTTATGTGCGCTTGCGCT10.5No Hit
GTGCAAAATACACCACGTCTACAAGACAGCAATGATCTGATAAGGACTTGA10.5No Hit
GTACGGGTGGTATCAACGCAGAGCTGTCTCTTATACACATCTCCGAGCCCA10.5No Hit
CCAAAATATAAGCAGCCCCAGGACCACCCACAAGAAGGCTGGGGTAGGTTT10.5No Hit
GATCTACAGCCATTCACTGCTGCCCATCATACGTTCAGGACCTGTCTCTTA10.5No Hit
CACCAGGCCATCTGCTTGAATGCTTCGGACACACTCCTCTAGTTTTTGTCA10.5No Hit
ATCCTTGTCATCTAGAACTACTTTGGTGCCTCCATATTCTGGGAGAAGAAC10.5No Hit
ACACTGGAGCTCTGTCGCGAGGGTCCAGGCGCCGGGCTGGAGGGTCTCCTC10.5No Hit
ACTTTTTACGAAACACATAAAAACCTCTATCAGTAGACCCTGTCTCTTATA10.5No Hit
CACCCCGGAAAGCTCCCCAGGGTGAGGATGAGCCCAAAGGGACTCAAGAGT10.5No Hit
GTGTTCACGTGTGATTTCAGGACCCGGACTTTAACCAGCGAGTTGAATGTA10.5No Hit
CTCGTTCTCTTTCTGCCGCCATCTTGGTTCCGTGATCTCCGCACAAAATGC10.5No Hit
CTGTAATGGCGGCTGTGACTGAAGTTGTGGTCCCCGCGGATGGCGCGGAAG10.5No Hit
GGCCACAGGCCTGCCCAACAGGGGCTTCCACAGCATCATGAGCTCCCCCAC10.5No Hit
CATGAAAGCTCTGGGCGTGGGCTTTGCCACTAGGCAAGTGGAAAACGTGAC10.5No Hit
ATGCTAGGTTTTGGATTGGACTTTGGGTCAGCTTCAGGGTAGTGAGGATGG10.5No Hit
TTACGATGGCATCTTCTGGTTCACTGAAGTGGCTGCTTGGGCTGTCTCTTA10.5No Hit
CTAATGCATTTCCCTAACCTAGTCACCTCGTTCCTAAAGGCTTTCCTACCC10.5No Hit
GTTTGAGTCATGTGGCAGCTTATTAAGTCCCATTTTTAAGTCAAAGACATT10.5No Hit
GTCATGCCCTGCCCTTGCTTGTGTCTTTCCTTCACAAGGTCAAGCCTGTGC10.5No Hit
GGATGGAAAGGAACAACATGGGTGGTGCGTCTGGAAACTAAAATTTGTTGG10.5No Hit
GGTGTGCACCTCGTTGCGGTACTGCCCGAGTCGGTTGCGTAGATCCTCCAT10.5No Hit
GGGAGTATGTGTTTAAAAGGACTCTCCTTCCTTTTTGTGTGTGTTTCCTTT10.5No Hit
CGCACCCACCGCACTCACTTTTCAAACAAAAGCCACATTCCCACTGTCCTT10.5No Hit
GGTAGGGAGGACTTGGGATTCATGAGCTATTTTTACTGGGAGACTGGGAGT10.5No Hit
ATTGTCAGCTGCTTTTATCAAATTCCAGGCCATTATCCAGCAAACACTATT10.5No Hit
TTCATAAATTGTGATGTGCAGGGGAAAGGTAAGACTGAAAAGGTAAAGCAC10.5No Hit
CTCCCGCAGGCTGCTGCGCAGCGTGAAGCGACGGCCGCACAGGTGGCAGGC10.5No Hit
GACCAAGGGTGTCACGCTGGGCTTCCGATACAAGATGCGGTCTGTGCACGC10.5No Hit
CATTGAGGCTATTTAAAACCTGGCGCTGTTAATACACAGTACTTCACTGTA10.5No Hit
CTTCAGTACAGTGCCAACAAATGCAGCCTGGGCTTTTCTAGCCACCCGACT10.5No Hit
AGATAATCATGTGGTTTTTGTCGTTGGTTCTGTTTATATGCTGGATTACGT10.5No Hit
CAGAGAACACACTACAAGAGCCTTTAGACGGCTGCCTTCCAGATAAAGTAC10.5No Hit
GTATAGATCTTAGATCCATTTTGAGTTAATCTTTGCATATGATCTGTCTCT10.5No Hit
GTGTGGCATGAAAACTAAGGGGAAGATAAAATTTCTACATGTGATACTGTG10.5No Hit
GAAATATTTGGAAAAACTCTACGTCGCTCCTGATAAAAATTCTCAACAAAC10.5No Hit
ATACACAAGGCTGTACCAGTGCAGGACCTCATTTTAATCCTCACTCTAAGA10.5No Hit
CCTCTGGGGAGGTTGCTGCCCAAAGTACAGGAGGTGGCCCGGTGCCTGAGG10.5No Hit
CATCTAAGTTGTGCACAATAAACTTAAAATGCTGTCTCTTATACACATCTC10.5No Hit
GGGCAGGCACGTGCCTTGGCACCACCCAAGTGACTGTTTTCTCTCACCGAG10.5No Hit
ATCTCAATCTGCGAGGTGTGATTTCTCATAGTAGGCCAGCGGGTCCTCTTC10.5No Hit
GATATAGTAAACCCTGGCTGACGTTGTCCTGTAGGTGTTAAAGAAGACTTC10.5No Hit
CACCCTGTCTGTCCATCAGTTGGTTGAGAACACGGATGAGACCTACTGCTG10.5No Hit
ACCTACGTCAGTCTCACATCTGAGAGCTTCTGCTACATACCTGTGACAGAC10.5No Hit
CTACTCAACTAACTTCATAATAGAAGCTCTACTACTATCATCAACATTCCT10.5No Hit
ACATTCAGAAGCTCTATAAAACTTCAATGAAGTACATTTCTCTGTTGGTGT10.5No Hit
GGTGAAAGAAGGCATGAACATTGTGGAAGCCATGGAGCGTTTTGGGTCCAG10.5No Hit
ATCCACTTCGAGCAGAAGGCAAGCGGTGAACCAGTTGTGTTGTCAGGACAA10.5No Hit
GTATGGAAAACAATCCAAGACAGCCAAGCTCCGGGTTTCTAGTGAGTAGGT10.5No Hit
ATGCTGAGGAGGCTGGGGTGAGAGCACAGTGAGGAGCGTGGGGAGTCACCT10.5No Hit
GTACTATCTGTTCCGCTAACCATATTCTTCCCCCAGGCCCGGGTCTGAGCC10.5No Hit
CCATTGTTCCCCTGTCAACAATGGTGCCTGGTTTTTTGGTCGCATCGTAGA10.5No Hit
GAAGTCATCCGGGAAAATTGTGTACTGCGGACAGGTGTTTGAGAAGTCACC10.5No Hit
AATTTACATCATAGTGGATAAAGTTTCTTTATGAGCTCTAGCCAATAGCAT10.5No Hit
GCTAAAGATAATCTCTCTTCCATAGCAATGGAAAAATCAAAGGTACCAAAC10.5No Hit
GGTCCTGCACTGGTACAGCCTTGTGTATTGTCCCCATACTGATGGACGTGG10.5No Hit
TGTTAGTACCACACTGTTTTGATTACTGTAGCTTTGTAATGTTCTGAAATT10.5No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTCCC10.5No Hit
ATATAAACCATTCTACCATAAAGACACATGCATACAAATGCTGTCTCTATA10.5No Hit
TAGTCATTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCC10.5No Hit
ATCAAGCTGGTTTCAAAGCACAGAGCCCAAGTAATTTACACCAGAAACACA10.5No Hit
GCACAGGTCAGACTCTTTCCAAGGCTTCTAACATGATGATGCCTGTCTCTT10.5No Hit
GTAATAATTCAGATAATCCTGAAGTTCATCCAGAAACAAAAACAGAAGACA10.5No Hit
AAAATGTACAAAACCACACCAGATGTCATCTTTGTATTTGGATTCAGAACC10.5No Hit
GACATAAGATGAGTCTCTCCTGAAATCTGTCATTTGCTAACTTGTTTTGAG10.5No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCAT10.5No Hit
TCGAGGAGGTGTGAGAACAATGGGCCTCTTCTCATCCAATGACAAACTCCA10.5No Hit
GGACTCGCCAGTGTTAGCTGCTGGAATGAGGTGTTTGTCCAGTACATCCAG10.5No Hit
GCTGATAGCAAGATGTCTTCAGGAAATGCAAAAATCTGTCTCTTATACACA10.5No Hit
CACATGGGCTTGCCAGGGACCATATCAACAATGGCAGCATCGCCAGACTTC10.5No Hit
AGTTACGAGTGCTTTCCTGATCCACATGAATTCCGAGGTTCAATCCTTAGC10.5No Hit
ATCCAGCGTAAATTCTACTAAGTGATAAGTTTGCTAGGACACAGGGAGATT10.5No Hit
CTACAGGATGGAAGCAGAGAAAAATCACCTGGCATTGAAGCCTGCACAGCC10.5No Hit
CACAGGAAACTGGTGATTAAAGAGGAAGGTGTTCTTTTTGAAACATTTGCC10.5No Hit
CCTCCAGTCTTCAAAAAAGTCAAGGTCGTGAAAGACTGATGATCTGTTTCA10.5No Hit
ACCTTGGCGGGGCACTGACCGAAGGAAGCACCGATTTGAGTGTGGAAGTAC10.5No Hit
GTTTTCTGCCTGTCTCTGCCTCCTGAGTGCTGGAATTAAAGATGTGGGACA10.5No Hit
AATCCATCTTGAGCTGATTTTTGTATAAGGTGGGAGAGGAGGATTCAGTTT10.5No Hit
CCCATGTGCTGGTGACCTGAGGAGGGGGCTTTTCTGGAAGAGAGCCCTGTC10.5No Hit
GTGAACAGCCTTCCTTGGTTATACTGCTGAGCAGGTCCTCCTGGTGCGGCG10.5No Hit
GTTAATATATATGTCACTGAACTGTTTTTAAAGAATCAAAGCCCCACAAAA10.5No Hit
GGCCGCGGGGAGCCTGGAACTAGATCAAGGCAGAGACAACTTTCAAATTCG10.5No Hit
CCCTTGATGAGCCTTGTGCGTCCTGTCTCTGGGTCCACCACCTTTCGAATA10.5No Hit
TCCATGTGCGTCTCCAGACCTGTGGTCCAGGGCGGCAGAAAGGCTTCCCGT10.5No Hit
ACTCTGGCAGACCCACTGGTGTTAGACACCACATCAGTCCTGTCTCTTATA10.5No Hit
AGAGAGAAAGAGAGAAAGAGAGAGAGAGAGAGAGAGCCTAAAAAGGGAAAA10.5No Hit
AATTCACACTCCAGGTTGTCTTTTAGTTCCACAGTTGGTGATTCCTGTATG10.5No Hit
GGACAACCACTGGGTCCTACATCGCCAATCGAGTGACTGACAGGCTGACCC10.5No Hit
CCACAGTAGGATGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGA10.5No Hit
GGTTTACTTCAGCAGCAGCTCGCTTCAAGATGCCGAAAGCTTGAATGACTG10.5No Hit
GTGTAACACCTTTACAGTCAGTGGTCTGGAGTAATGCCACTGACCACTCCT10.5No Hit
ATGCTAGACCACCTCAAGCTCCACTCCTATGCCACTTCCATCCGCAAAGCT10.5No Hit
TCTCTCAGTACATCAAGAACAACGTAACTCCAGACATGGAGGAGATGTATA10.5No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT10.5Illumina PCR Primer Index 8 (95% over 21bp)
GGTCTACAGAGTGAGTTCCAGTACATCTAGGAACACAGAGAAAACCTGTCT10.5No Hit
CACTGAGGGCGGGACGGACCCAAAACCCCGACGAGCCCCGGCCACCGAGAG10.5No Hit
GTGACCTGCTCTTCAGCAGGCCCAGCCAGTACAGAATCCCAACAGGTGCAA10.5No Hit
GTATAAGGTCATTCATCTTAAAAACCTCACACACTACAATCTGTCTCTTAT10.5No Hit
CGCTGGACTCGTTCGATGTAGATGACGTACTTCTTCCTGTACACTTGGACC10.5No Hit
GTCCTCCAGAAGTGATGCTCGTCTTTGCTCAACTTGATGGGTCCTTGCACA10.5No Hit
CAGTAGCACCAAGGACACCATATGTGGCCACCTGAAGGTGGTGGTGACTCA10.5No Hit
AATCTAGGATGTGATTTGTAATCAAAAGTTTAGTCATAACCTAAGACTTCT10.5No Hit
CTTATACACATCTCCGAGCCCACGAGCCCATTCCGAATCTCGTATGCCGTC10.5RNA PCR Primer, Index 46 (95% over 21bp)
AGTCAAGGTTGCGCTGAACTTTCCCATAACCATTATCCCCCAAGACCACCA10.5No Hit
AAACAAAGGGGTGAAAACAAGAAAGAATAGGATGGAATCTGGAATAAACAG10.5No Hit
ACATAATTCTGACCTTTCGGGCCATAGACGGATGCACAGAAACAACTCTTG10.5No Hit
GACTGGAAGTACCTGGTGAGCTCTGGGGTAAGCCTAGATATCTTTACATTT10.5No Hit
GGGGAGGGTATGTTAACATGTTTAAACCTTTGGACAGGAGAGAGACAGAGA10.5No Hit
GATTGTTAGCTTTTTCTGTTTTATTTCTTTTGAGAACATTTGAATCTGTCT10.5No Hit
ATCATAAGACATTTTCTAGTCTTTTGGCAGGATCAACATGTGTAACTGCCT10.5No Hit
ACATTGCATTTACTGCCTAAGAGCTAGTAGATTAACTGGCTGACAAGAATG10.5No Hit
TCACGATACATACTATGTAGTAGCCCATTTCCACTATGTTCTATCCTGTCT10.5No Hit
CCTCAGATATGGTCCTCAGCTAAAAGAAGGCTGCAGGCAGCTCAGCTCTCT10.5No Hit
CTCGTTCGCTCGGCCCTTATTCTGATACTCCACGATACAGCGGATCAGCTG10.5No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTGGTATGCCGTCT10.5No Hit
CGGAGGGAGAAGTCGATGTGCTTCTGCGAGTCCAGGCGAACAATAAAGGCT10.5No Hit
GATATGTAGTGTTTACATTACAATAAAAAGCTAGAACTATAGTCAGGCCAC10.5No Hit
CTAAAGTGCTTATTACCTCACAAGCTTGACAATCTGAAATTTGATTCCCAG10.5No Hit
ACGAGGGAGTGCGCGGCACCTGCGAAGATGCTTCTCTCTGCAAGAGGTTTG10.5No Hit
CATGGGCAATGAGAAACATTCACTTGACGACTTGAAAAAATGACCTGTCTC10.5No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers