FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_45.34100000007166.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_45.34100000007166.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271843
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC41021.5089592154294942RNA PCR Primer, Index 46 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT4560.16774388157870537RNA PCR Primer, Index 18 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAT250.002104285236.06
TGCCGTC5400.035.41666845
AATCTCG5650.034.24778736
TTCCGAA5700.033.9473731
TCTCGTA5700.033.9473738
CGAGACC5700.033.9473723
ATCTCGT5750.033.65217237
GCCCACG5750.033.65217218
CCGAATC5750.033.65217233
ATGCCGT5700.033.5526344
CCACGAG5850.033.46153620
CTCGTAT5800.033.3620739
CATTCCG5800.033.3620729
CGAGCCC5800.033.3620715
TCCGAAT5850.033.0769232
CCCACGA5850.033.0769219
ATTCCGA5850.033.0769230
CGAATCT5850.033.0769234
ACGAGAC5850.033.0769222
TATGCCG5800.032.97413643
Produced by FastQC (version 0.11.2)