FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_44.3410000000715a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_44.3410000000715a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386505
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC41551.0750184344316374RNA PCR Primer, Index 46 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT4980.12884697481274499RNA PCR Primer, Index 18 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC5450.037.1551122
CGAGCCC5500.036.822115
TCCGAAT5350.036.58794432
CTCGTAT5500.036.40825339
CGAGACC5450.036.3294423
CCGAATC5400.036.2491733
TATGCCG5550.036.0802543
TCTCGTA5550.036.0802538
CGAGTTG250.002105620235.9991729
AATCTCG5450.035.91660736
GCCCACG5650.035.83988618
CACGAGA5650.035.83988621
ATGCCGT5600.035.75810644
ATCTCGT5550.035.67485437
CATTCCG5500.035.5900929
ATTCCGA5500.035.5900930
CCCACGA5700.035.525519
CGTATGC5650.035.44166641
TTCCGAA5650.035.04344631
CGAATCT5600.034.9545534
Produced by FastQC (version 0.11.2)