FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_43.3410000000714d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_43.3410000000714d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525370
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC29810.5674096351143003RNA PCR Primer, Index 46 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA3150.037.8562638
AATCTCG3150.037.8562636
TATGCCG3100.037.7410643
TCCGAAT3300.036.81732632
CTCGTAT3300.036.13552539
ACGAGAC3300.036.13552522
CGTATGC3400.035.73446341
CCGAATC3400.035.73446333
TCGTATG3450.035.2165740
CATTCCG3400.035.07605429
ATTCCGA3400.035.07605430
TTCCGAA3400.035.07271631
CACGAGA3500.034.71347821
CGAGCCC3450.034.5644115
ATGCCGT3400.034.41096544
TGCCGTC3450.033.91225445
ATCTCGT3550.033.59076737
ATCTCCG3550.033.59076710
CTCCGAG3600.033.1242312
TCTCCGA3600.033.1242311