Basic Statistics
Measure | Value |
---|---|
Filename | H23NVBCXX l02n01 12h_43.3410000000714d.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 525370 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC | 2981 | 0.5674096351143003 | RNA PCR Primer, Index 46 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCTCGTA | 315 | 0.0 | 37.85626 | 38 |
AATCTCG | 315 | 0.0 | 37.85626 | 36 |
TATGCCG | 310 | 0.0 | 37.74106 | 43 |
TCCGAAT | 330 | 0.0 | 36.817326 | 32 |
CTCGTAT | 330 | 0.0 | 36.135525 | 39 |
ACGAGAC | 330 | 0.0 | 36.135525 | 22 |
CGTATGC | 340 | 0.0 | 35.734463 | 41 |
CCGAATC | 340 | 0.0 | 35.734463 | 33 |
TCGTATG | 345 | 0.0 | 35.21657 | 40 |
CATTCCG | 340 | 0.0 | 35.076054 | 29 |
ATTCCGA | 340 | 0.0 | 35.076054 | 30 |
TTCCGAA | 340 | 0.0 | 35.072716 | 31 |
CACGAGA | 350 | 0.0 | 34.713478 | 21 |
CGAGCCC | 345 | 0.0 | 34.56441 | 15 |
ATGCCGT | 340 | 0.0 | 34.410965 | 44 |
TGCCGTC | 345 | 0.0 | 33.912254 | 45 |
ATCTCGT | 355 | 0.0 | 33.590767 | 37 |
ATCTCCG | 355 | 0.0 | 33.590767 | 10 |
CTCCGAG | 360 | 0.0 | 33.12423 | 12 |
TCTCCGA | 360 | 0.0 | 33.12423 | 11 |