FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_42.34100000007130.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_42.34100000007130.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482418
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC23260.48215448013962997RNA PCR Primer, Index 46 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATC3350.033.58154733
CACGAGA3600.032.49947421
ACGAGAC3600.032.49947422
CCACGAG3650.032.05427620
CGTATGC3600.031.87448541
TCGTATG3700.031.6211140
CGAGCCC3700.031.6211115
CTCGTAT3650.031.43784939
TATGCCG3650.031.43784943
AATCTCG3650.031.43784936
TCTCGTA3750.031.19949738
CATTCCG3650.030.8214229
CGAGACC3750.030.59950623
ATGCCGT3750.030.59950644
GCCCACG3900.029.99951618
TCCGAAT3750.029.99951632
CCCACGA3900.029.99951619
ATTCCGA3750.029.99951630
CGAATCT3750.029.99951634
TGCCGTC3900.029.99951645
Produced by FastQC (version 0.11.2)