FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_41.34100000007123.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_41.34100000007123.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237752
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC20850.876964231636327RNA PCR Primer, Index 46 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT3360.14132373229247283RNA PCR Primer, Index 18 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACC250.00210414235.9986534
TCTCGTA3200.035.15493838
TGCCGTC3200.035.15493845
TATGCCG3250.034.6140943
CCGAATC3250.034.6140933
TCTCCGA3350.034.2596611
GCCCACG3350.034.25245318
CGAGCCC3350.034.25245315
TCCGAGC3400.033.74873713
CCCACGA3400.033.74873719
ACGAGAC3400.033.74873722
CGAATCT3350.033.58083334
ATGCCGT3350.033.58083344
ATCTCCG3500.033.43435310
ATCTCGT3450.033.2596337
CTCCGAG3450.033.2596312
CCACGAG3450.033.2596320
CTCGTAT3400.033.08699839
TCCGAAT3400.033.08699832
ATTCCGA3400.033.08699830
Produced by FastQC (version 0.11.2)