FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_40.34100000007116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_40.34100000007116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293676
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC16730.5696754246176058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1700.033.08698343
ATGCCGT1750.032.14164444
CGACCTA1700.031.76350631
TATCTCG1800.031.24881736
ACGAGAC1800.031.24881722
TGCCGTC1800.031.24881745
TGCGACC1750.030.8612329
ATCTCCG1850.030.40425510
CGTATGC1850.030.40425541
TCTCGTA1850.030.40425538
CGGAGAT453.47086E-530.0141941
GCGACCT1800.030.00397130
CTGCGAC1800.030.00397128
CGAGACT1900.029.60414323
GACTGCG1950.028.84997626
ATCTCGT1950.028.84506237
CTCGTAT1950.028.84506239
CACGAGA1950.028.84506221
CCGAGCC1950.028.84506214
CGAGCCC1950.028.84506215
Produced by FastQC (version 0.11.2)