FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_38.341000000070fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_38.341000000070fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC2213.414634146341465No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC63.6585365853658534No Hit
TGCCTACACTTATCAACTGGTGCCACGTATTCCTCAAAATGCCAAGATTAT10.6097560975609756No Hit
ACCTTACACAAACACAAGTAGCAGTCACAAAACAGAAAAGAAAACTTTGGG10.6097560975609756No Hit
GGGTTTCTCTGTAGCCCTTGCTGTCCTGGAACTCACTCTGTAGTCCAAGCT10.6097560975609756No Hit
GTACTTCCAGGCTTCCCTGGGATATACCAAGACATGGAGTTCAATTTTTGC10.6097560975609756No Hit
CCATATTAGAGGATAAGTATACCGTGTATAAGAACAGACCTATCTCCTCTT10.6097560975609756No Hit
TCTTGACACTCCTGCTACAGCATGCTTGTGACCCAACAGGTCTTTAGTTCT10.6097560975609756No Hit
CTATAGTCTAGCTCTTTGGGGACCCACCAGACTGTGCCTTGGGAATTATAG10.6097560975609756No Hit
GAGGCTCTCCAGGCTGGTGCAGACATCTCCATGATCTGTCTCTTATACACA10.6097560975609756No Hit
GCCCAGATTCCACCTCTCCTTCTACCAGCCCTACCTTCTGGAATTACAGTC10.6097560975609756No Hit
CTTTTAGGAGATGACCAAATTTACAATGTTATCGTAACCTGTCTCTTATAC10.6097560975609756No Hit
GCGAATGACTGAGGCCATTCCGGTCGCCGAACCGAGAACAAGAGCGCGAGC10.6097560975609756No Hit
GCAGAGCCTTATTATCTAATACACACGCCTTTCAGAAATTCAGCGTGTATT10.6097560975609756No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT10.6097560975609756Illumina PCR Primer Index 8 (95% over 21bp)
CGTTTGTTGTCTGTCGGTTTGTTTAGTGGAGTTTGGTGGATGCCCTTTGCA10.6097560975609756No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATATCGTATGACGTC10.6097560975609756No Hit
TCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCATTTTTC10.6097560975609756No Hit
CTACTGTGTAGCATAAGCTAGTGTCAAACTTGTGATCCTTTTGCCTGTGCA10.6097560975609756No Hit
CTCTTCTAGGTTTCCAGTTTATGAAGCCCCCATTTTGTCATTTAAATTCTT10.6097560975609756No Hit
GTCTTTGTACTTTCTTCATTTCCACCTTTGCCCAAGTCATCTTGTTTCTCA10.6097560975609756No Hit
TCTCATGGCAGGTTAAAACAAACAACAACAAAACCCAACTTGACATCACAG10.6097560975609756No Hit
GTATGGATTCTGTGATGGAGAATAAGATGGTAGCCCCGACTAAAGGTCTTC10.6097560975609756No Hit
CAGTAATTTGTTGAAGAGTTAACAAGAAAAGGTCTTTCTAGATTGTGTCCT10.6097560975609756No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT10.6097560975609756RNA PCR Primer, Index 44 (95% over 22bp)
CCCTTTTCTGTGAAAAAGACTAAAAGTATCTTCAACAGCGCCATGCAAGAG10.6097560975609756No Hit
GATCAAATACTGCATAGAAACAAACTACAAGAGAAAAATTCGGGAACTACC10.6097560975609756No Hit
AACAAAACAATGTACAAAGTCCTCAGCCACATTTGTAGAACTTTGGGGGAT10.6097560975609756No Hit
GCTCATATTGTCCTGGAAAAAATGTCTGTATTGAGAAATCCTAAAGAGCCT10.6097560975609756No Hit
TTTCTCTCCTGGTGTCTTTTAACAAAATGAAGAAGCTGACAACTGATGGGA10.6097560975609756No Hit
CACACACATACATACATACACACACACACACATATTACTCATCCTACACTG10.6097560975609756No Hit
CTCTTGTATTCTGTTGCTGATGCTCAAATCTATGGTTCCAGATTTCTTTCC10.6097560975609756No Hit
CATGCATACTAGGCAAGCTTTGTACCACCTGAACCACCAATCCATTATTGC10.6097560975609756No Hit
GTCTACAGCTGAGTGTGACCATTGATATGTTTTCTCTCCCCTAGAAGTCTG10.6097560975609756No Hit
GCAGAGGCACTTGGCAATTTTGGATATGAAAGAGGGTAAGAAGTGTAACTG10.6097560975609756No Hit
GGAATTAGGGCCTAGATTGTAAGCCCTGTGGCAGTTGAATAACGTTCATTC10.6097560975609756No Hit
ATTTAACATTGTCCACTCATTTGCCATACTGACTGTGCAAATACAAATCTG10.6097560975609756No Hit
CATGCCTACAGTTCAGCTATCTTGGAGCCTATGACAGTGAACTTCTTGAAC10.6097560975609756No Hit
ATACCACATTTACAGAATGGAGGACAAAAATGACATAATCCTGTCTCTTAT10.6097560975609756No Hit
GCGGAGAATCAGAAGCTGGACTTTAGTACTGGAAACTTCAACGTGTTGGCC10.6097560975609756No Hit
TTTTTACCACTGTACAAAGGTACAAAAATCATACTTTAGGGAAAACCATTC10.6097560975609756No Hit
ACTTTTAATAAAGGGAAAAGTAGGATAATGATGAAAAACCTGTCTCTTATA10.6097560975609756No Hit
CAGTAACAACAAAGAAAAGAGAAAGAGAGAGAGAGAGAGAGCTGTCTCTTA10.6097560975609756No Hit
ATCCTCCCTTCGGTGGTTTTCCAGCCTAGGATGTTCCCAGGGTGTCTAATC10.6097560975609756No Hit
TTGAAGAGCTTGACTCCTGGGATCTATATACCTTGCTTCTGGAATGCTCTG10.6097560975609756No Hit
TGTATTTTGAAGATTGATGTTTGCATTTTATTTCAGTAAACAATTGCTAAA10.6097560975609756No Hit
ATCCTACTGTGAATATGTGGTGGGCTCATACAATAAAGCCTAGAAAGCCAA10.6097560975609756No Hit
CTTTAAGACCATGATAGTGGAGTAAGGGAAAGAGTGAGATGTAGCGCTCTG10.6097560975609756No Hit
GTGCTTGGATAGTCAGTTGGTTATTTGTGTATGAAACAATGTACAAATCCT10.6097560975609756No Hit
ATCAAATTGATGCTGGTTAGCTGGAGCTAAGAAATTAGCACTGATTAAGAA10.6097560975609756No Hit
CGGCTACTATGGCAGGCGCCGTGGACCTCCCCGTAATTACGCTGGGGAGGA10.6097560975609756No Hit
GTACTCTCTGTTCATGAACTGACAATAATGAAGATACTGAGGCTATAGCTG10.6097560975609756No Hit
GCTGTGACTGGTCCACAATTCCTTTCTTGTCATCACCAGCAGCTGTCTCTT10.6097560975609756No Hit
CAATCATTAGGAGTTGAGGAGTACTGAATCCATTACTACTCTGATGACACA10.6097560975609756No Hit
GTACTAGTTACCATTTGTACAAACTCAGTCACTTTGGTTTGTTGTAACTGC10.6097560975609756No Hit
TTGCCAAACACTTTTAAACCAGTGAGCTCCAAGGCCTTTTCTAGGCTGTCT10.6097560975609756No Hit
TCACCTTGCTGCAAATACATCATTTTTAAGCCATAATCTTCCACCTGTCTC10.6097560975609756No Hit
GATCATGATGAGAACAGCTGTTAGTGAAATAAATGATCCTATAGCTGTCTC10.6097560975609756No Hit
GCTTGAGGGAGGGGCAGGCGCAGCCGCGGGCCCGCGGGGAGCGCGCCGTTG10.6097560975609756No Hit
GCTCATTCTTGCCGACTCCTCCCCCTCTGAAGCAGGTTAGCATTGAAGGTG10.6097560975609756No Hit
GTGCAGGGAACCATCCACTTCGAGCAGAAGGCAAGCGGTGAACCAGTTGTG10.6097560975609756No Hit
GTCATGGCCTTGTTATTGGAATGCTTGTACATCTTTAATCTTGCTTCAATA10.6097560975609756No Hit
ATTCTGAACACTTATTTCTAAGGTTCACTTTTCAGTGACTGACAGGACATT10.6097560975609756No Hit
GAGTAGTGTAGTTCTGAGTCACTCAGGGTTTCATCCCTAGTCTTTTGCTGT10.6097560975609756No Hit
GAAATATGGTGAGGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACT10.6097560975609756No Hit
CGGGGAAGCATGGCGATGAAAGCGGTGTGCGTGCTGAAGGGCGACGGTCCG10.6097560975609756No Hit
CCATATGCCAGTCCCCATGGAACAGAAGACGGCTCTTATCCCACCAGGGCA10.6097560975609756No Hit
GCCCAAGCCCCCACAGGCTAAGCAGGGGGCCGTGACTGTCTGTCTTGGGGA10.6097560975609756No Hit
GAATAAGGAGTGAGCTACATACTAAGTCACTAAGACACCACAACTCACACA10.6097560975609756No Hit
GTGCGGAAGCGGGGCTTCGAGGTGGTGTATATGACTGAGCCTATTGACGAG10.6097560975609756No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGC10.6097560975609756No Hit
ATTTAAGTCCTTTGCTCATTTTTGGGTGTTTTTGTTTTTGTTTTTGTTTTT10.6097560975609756No Hit
TAATTCATCAGTAGGGCATCTCGCAGCTTGCATAGTCCGGCCCGCCATCTT10.6097560975609756No Hit
TGGTAACCTGCCCTATGATGTGACTTCCACTGAGCTCCTGGACACCCACCT10.6097560975609756No Hit
CTGATCAATATTGAGCCTATATAAACAACAACAACAAAAAAGCTTGATTAA10.6097560975609756No Hit
CCAAGAAACACAACCAAAACCCCCCCACAAACCAAAAAAGTCCACAGAGAA10.6097560975609756No Hit
AAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTGTCTCTTATACCA10.6097560975609756No Hit
GTCCTTTACTAAGGAGCTCCTGAAGGGCTGCCCTGGCCAAGGAACTGCGAA10.6097560975609756No Hit
GTATATCAGGGAGGAAAAGCTGCTGGGTAACCTGAGCAGGTATCAACCCTC10.6097560975609756No Hit
GGGTAATCCAATGCTACTTCAGCTTTCTAGTCTTCATAGTTACATATCTAC10.6097560975609756No Hit
CCCTGGTTCAGTTTTATAATTTCTAGAGGCTATATATCATAGTTCAAGAAC10.6097560975609756No Hit
GCGTCAGATGTGAATAAGAGACAGGGAATATGGAGAGTTCTACAAGAGATT10.6097560975609756No Hit
GTGGTGGCTCATCCAGCTACAGCTCCTACTACCAAGGAGACAGCTACAACT10.6097560975609756No Hit
GCCTAGACTGAGCTGAGGGCAGTGGGAAGGAGTTGGGTTCTGATAGACAGA10.6097560975609756No Hit
CCTCAAGGCCATGCTGCTTTCTGAGTTGTTCTTGTTGCTTTGTTCATAATG10.6097560975609756No Hit
GGGCGGGGATGCACATCCGCTGGTCCGCCTCGAGAATGTTGATTTACCTGC10.6097560975609756No Hit
CAACATGGCTTCATCCTGAGACTCGGGAAAAACCCAGAGAGAAGATAGAGA10.6097560975609756No Hit
CAACAGGTCCTGAGTGTTCCTATCACAGTAGGCACACATAGCCAGGGATGG10.6097560975609756No Hit
TCATCACAGCGGCCAGCCTGGTGTGCCGGAAACGAAAGGGCACCGAGGTGC10.6097560975609756No Hit
TAACGGAAGCGTGGATAAGATGCTCGGATCCATAGGAATGTTGATGATAGT10.6097560975609756No Hit
CTGTTGATCTTGCGCAGGCTACGCACCTCATCCTCCAGATCAAACACACGT10.6097560975609756No Hit
ATAAATTTGGGGGGAAGTATCTTGAGCAAAATACAACAGGAAAAGAATCTC10.6097560975609756No Hit
AGCATACCTGAATAAATCTATTCAGTGAGTTACCTACCTGTCTGTACACAT10.6097560975609756No Hit
GTTCTAAGGTCTCTAACTCTGAATAATGTCTAGCTGTGGGCTTCTGCATTT10.6097560975609756No Hit
GGGCCCGCGTGTGTCCTGTGTGCTGTGGTGGGTGCAGCGGTGCGGTCGGCG10.6097560975609756No Hit
GGCTATATCGGCGTGGTTAACCGAAGCCAGAAAGACATCGAGGGCAAAAAG10.6097560975609756No Hit
TCCAAAGATTGACTTTTAAGTTTGGTTTTAGAAAGTTAACTTTCATTTCAG10.6097560975609756No Hit
ACTTTACATCAGCCACTATAATTATCGCAATTCCTACCGGTGTCAAAGTAT10.6097560975609756No Hit
CAGTAGCATTTGTGCCAATTTCTGGTTGGAATGGTGACAACATGCTGGAGC10.6097560975609756No Hit
CACGGAGGCCATACCGGCTGGCGCGTCGAGTTTCGCTCAATGGAGTTTCAG10.6097560975609756No Hit
GTGAAACGTGGTTATGAGGCTGGTTTTGGTGACGGACCACCTTCTAATCCC10.6097560975609756No Hit
AAAGAGAACACTGAACAGTCGTATATCTTCTTAACCAAAGACTTGGGACAC10.6097560975609756No Hit
GCCATGAGAGGGGAGGAGGTAGAGAAGCAGAAGGTAATGGCTACTCCACGC10.6097560975609756No Hit
CTTAAATCCAATTTAAAAGGTGTTTGGTTGTTTCCATAACATTAATGCCTG10.6097560975609756No Hit
GGCTTTTATTGAAGGCCTACTGCGTGTTGAGCACCAGGTATGCCTCCCATC10.6097560975609756No Hit
ATGAAAAGGACAGCCCTGTGCACTCCTCTTCATCTGTCTGGGCTCAGCATG10.6097560975609756No Hit
CCACCATACAGTCAGAAGAGATACGAGGAAATCGTTAAGGAAGTCAGCACC10.6097560975609756No Hit
GCCACTGAGCTAGTTTTTAACTTCAGTCCAAAATTTCATCTACAAACAGGT10.6097560975609756No Hit
GCTGTAGATTCTAGCTAGGCCAAAGTCAGCCAGCTTGACGGTCCCATTACT10.6097560975609756No Hit
GATGAGGACATCCCACTGTTTCCAGTGCAGTCCAAGTACATGGATGTGGTC10.6097560975609756No Hit
GTAAGAAATTCCTTAATGAAAGTAGGTGCTAGTAAAAAATCTGTCTCTTAT10.6097560975609756No Hit
ATCCGTGGCTGCTCATTCTTGCCAACTCCTCCCCCTCTGAAGCAGGTTAGC10.6097560975609756No Hit
GTTGATGGGGCCTTAGCAGTTATAGGCTCTGCATCTTCCTAAACATTCAGA10.6097560975609756No Hit
CTCCTCGTCCCCATTCTAATCGCCATAGCCTGTCTCTTATACACATCTCCG10.6097560975609756No Hit
CCAGTATGCAGCCAACTGGCATCAGTGTCTCCTACCCAAAGACAGTGGAGC10.6097560975609756No Hit
ACTCTAAACAGCTTCAGGAAGTTCCTAAAACTGACAAGACAAAGTCTCTCC10.6097560975609756No Hit
AACGTATCCCCAGCTAAAATAATAGATTGGTATCCTGATGCAATTGCACAG10.6097560975609756No Hit
ATGGTGGTCCATGAGAAACAAGATGACTTGGGCAAAGGTGGAAATGAAGAA10.6097560975609756No Hit
CATAAGCCCACATCGGACATATTTCTGGCCCATGGTTTCCCTGATCTTGTC10.6097560975609756No Hit
GTCAACTACAAAGGTGGCCTGAGTGAGCCTGGTAGACTGGACCAGGCCCCA10.6097560975609756No Hit
ATTGAGAACTGCCAAAGAATTCTTTAATTGATGCAATAACTCTGTCTCTTA10.6097560975609756No Hit
GAATTAAGTAAAGCCAAATATTCCAGGAATATCGTTAAGAAATTTCTCATG10.6097560975609756No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT10.6097560975609756Illumina PCR Primer Index 8 (95% over 21bp)
GCATGTAAGAGTTTATTTGTAAAGTACTTTGAATGTAACTCTTACATGCTT10.6097560975609756No Hit
GACTTCGGGGTGGTGCTAAGAAAAGGAAGAAGAAGTCTTACACCACTCCCA10.6097560975609756No Hit
ATATTGCACTTTAAGAGGAACTAATTTCTAGGCTTTTCATCAAAGAAGGAA10.6097560975609756No Hit
GATAATAGGAGTGGATGAGAATGAAGATTTTCAACTCAAAGGTCCAGCAAA10.6097560975609756No Hit
GTCAAGAAATGAATGGATTTGGATAGCTGCTGGGGACTCACTGTCTAGCAG10.6097560975609756No Hit
ACCACGATGACTCAGAAAAGGTGCCTAAAAGACTATATTCCTCCTGGGCCA10.6097560975609756No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTGCTGGAATCTCGTATGCCGT10.6097560975609756No Hit
ACAGAGAGACAGAGAGTCAGACAGACAGAGTTAGAGACAGACAGAGACAGA10.6097560975609756No Hit
CTCCACGGGTGTGTCACTGCAGTGGCACCTAAGAAGCCGCTGTGGAAACCT10.6097560975609756No Hit
GTACAGTGGAGAACTGTGAGCAAGTGAACACTGAAAGTGAGACAGCTGTGG10.6097560975609756No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGAATCTCGTATGCCGTC10.6097560975609756No Hit
ATATTCTGGAATGTGCATTGACTTCGATGTAGTGTACCTGTCTCTTATACA10.6097560975609756No Hit
GTTAAAATCCCCAAACACCTGACTGACGCTTACTTCAAGAAGAAGCAGCTG10.6097560975609756No Hit
GTGTTATGTGGAAAGATGGAATGGTAGAGGGGCAAAAAGAAGAAAATTCAT10.6097560975609756No Hit
TAGTTGTTTAGTTCTTTATCCAAGTCATCTAATAAGGAAATTGCCTGAACA10.6097560975609756No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers