FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_36.341000000070d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_36.341000000070d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3020
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC163554.13907284768212No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT2498.245033112582782Illumina Paired End PCR Primer 2 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCTTC1033.410596026490066Illumina PCR Primer Index 4 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCTT441.456953642384106Illumina PCR Primer Index 4 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC260.8609271523178808No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC230.7615894039735099No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT190.6291390728476821No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC190.6291390728476821No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC190.6291390728476821TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC140.4635761589403974No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC70.2317880794701987No Hit
CTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT60.1986754966887417Illumina Paired End PCR Primer 2 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.16556291390728478No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTAGTATGCCGTC50.16556291390728478No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGGCCTATCTCGTATGCCGTC40.13245033112582782No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG40.13245033112582782No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT40.13245033112582782RNA PCR Primer, Index 27 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCTTCTG40.13245033112582782Illumina PCR Primer Index 4 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC1600.043.593751
TTATACA2050.034.024392
GCCGTCT800.033.7545
TATACAC2100.033.2142873
ATACACA2200.030.681824
ATCTCCG2250.030.010
CACATCT2250.030.07
CATCTCC2250.030.09
ACATCTC2250.030.08
TACACAT2250.030.05
ACACATC2250.030.06
ATCTCGT2200.029.65909237
TCTCGTA2200.029.65909238
CTCGTAT2200.029.65909239
TATCTCG2050.029.63414636
CTATCTC2050.029.63414635
AGCCCAC2300.029.34782417
GCCCACG2300.029.34782418
CCACGAG2300.029.34782420
GAGCCCA2300.029.34782416
Produced by FastQC (version 0.11.2)