FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_35.341000000070c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_35.341000000070c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310923
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC40171.2919597456605012No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT4940.15888178101973802Illumina Paired End PCR Primer 2 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5600.036.5622445
CGACCTA5550.036.48622531
CCCACGA5700.036.3155319
CGAGACT5700.036.3155323
TGCGACC5650.036.23867829
GACTGCG5650.036.23867826
CTGCGAC5700.035.92079528
ACGAGAC5700.035.92079522
TATCTCG5600.035.75867536
CGAGCCC5800.035.689415
TCTCCGA5800.035.689411
ACTGCGA5750.035.6084427
TATGCCG5750.035.6084443
TCTCGTA5750.035.6084438
GCCCACG5900.035.46584718
CCGAGCC5850.035.38436514
TCGTATG5850.035.38436540
GCGACCT5800.035.3014730
ATCTCGT5750.035.21713637
ATCTCCG5900.035.08449610
Produced by FastQC (version 0.11.2)