FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_34.341000000070b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_34.341000000070b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498990
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC17020.3410889997795547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2750.032.72545243
TGCGACC2800.032.1507329
ACTGCGA2750.031.91690827
TCGTATG2900.031.80857740
TATCTCG3000.031.49824736
CGACCTA2800.031.3375431
GCCCACG2950.031.26944718
TCTCGTA2950.031.26944738
ACGAGAC2950.031.26944722
TGCCGTC2950.031.26944745
CTGCGAC2850.030.79701628
ATGCCGT2950.030.50677744
CCACGAG3100.030.48217220
GCGACCT2900.030.26603530
CACGAGA3050.030.24421921
CGAGCCC3050.030.24421915
ATCTCGT3100.029.75640537
CCCACGA3100.029.75640519
GACTGCG2950.029.75305226
CCGAGCC3200.029.52960814
Produced by FastQC (version 0.11.2)