FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_33.341000000070ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_33.341000000070ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286892
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC7340.25584540523960236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3180.11084310472233455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG754.7293724E-1132.99872210
CGAGACT801.0004442E-1030.93630423
CGACCTA751.6934791E-929.99883831
TATGCCG751.6934791E-929.99883843
ATCGTCG300.005141870629.99883844
CCGAGCC901.2732926E-1129.99883714
CGAGCCC901.2732926E-1129.99883715
GCCCACG852.0190782E-1029.11651818
CACGAGA952.3646862E-1128.41995221
ACTGCGA803.3669494E-928.12881527
GCGACCT803.3669494E-928.12881530
GACTGCG903.9108272E-1027.50372926
TCGTATG903.929017E-1027.49893440
ACGAGAC903.929017E-1027.49893422
GTATCAA2000.027.0130791
ACCTATC856.4410415E-926.46956333
CTCGTAT957.366907E-1026.05162439
TCCGAGC957.366907E-1026.05162413
CCCACGA957.366907E-1026.05162419
TATCTCG957.366907E-1026.05162436
Produced by FastQC (version 0.11.2)