FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_32.3410000000709e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_32.3410000000709e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC74.487179487179487No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC63.8461538461538463No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC63.8461538461538463No Hit
CCACTGGTCACATTTCTACTAAATCTTTCTGTAACACTTCCTGTCTCTTAT10.641025641025641No Hit
GCCCTGGACTGGGCGAAGTGGGAGTACTGGGTTCTGATGATGGTGAGTGGT10.641025641025641No Hit
TGCTTATTTGGTCCTTTAACCAGTAAAGTGACAGAACGGGGATTGTTACAC10.641025641025641No Hit
TGGTACTAGTTACCATTTGTACAAACTCAGTCACTTTGGTTTGTTGTAACT10.641025641025641No Hit
AAGCTAGACTTCGTGCAAAACCAAATACTCTACCTGTCTCTTATCACATCT10.641025641025641No Hit
CTCCCTGGCCAGAAACTGGCCGGATGGGCCCTAGATCTGTCTCTTATACAC10.641025641025641No Hit
AGGCAGCTATCCCCTAAAAGCTGAGGATGTCTGACAGCCCATAAAACTGCA10.641025641025641No Hit
TCTCTAGCTTCTCGGTGTTCTGTTTCTCTTTCTCCTTTGTAGTTCTGGGGA10.641025641025641No Hit
TACCAGTGTCCCCTCTAACCTGCCTTTGGATACGCATCACCCCAGCTGACT10.641025641025641No Hit
TGGCATGAGTGGTGACATCTTGGGGTGATAGCTGGGGGGATTCATTGGGCT10.641025641025641No Hit
GCGACAGTGGGTGGTTTTTTTGTTTTTTGTTTTTCTTTTGTGTGTGTGTGT10.641025641025641No Hit
GGATAAGCCAGGATGCTCGCCTTTGATTTTGGGGCGGTACTCAGAACAGAA10.641025641025641No Hit
TTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCAGATTTTTGAGTTCGAGG10.641025641025641No Hit
CTTCACAATGCTTCAAAATTCAAAAATAAAGGAAAAGAGAATTCGATGAGA10.641025641025641No Hit
ATCAAGTACTACTCAACTATTAAAAACAATGACTTCAGACTGGAGAGATGG10.641025641025641No Hit
GATGAGCCCCGCTGCCCTGGCCCCCGGCGCCCCCGGAGCTGTGTCGAGGGA10.641025641025641No Hit
CCCCGCGATCGTTCTGAAACTCGAGGAGACGCTCCGCGCCCTCGCGCTTCT10.641025641025641No Hit
GTTTTGTTTGGTAACTGTTGAGGAATAAAGGCTTTTTAACCCATTTTTGAA10.641025641025641No Hit
ACAGTGACTGCAGAAAATGCTGGGTAGTAATCAGTCATATTTTGAACACTT10.641025641025641No Hit
AGTCAAAGCCATCCAAAAAGACCGTGGGAGTGAAGAAGACAGCAACCAAAA10.641025641025641No Hit
GCGCCATGTGCTGAGGCTGTCTCCTGCTTCAAACTCTCTGCTACCTCCACA10.641025641025641No Hit
CCCCTCCTTCCCTGCACATATGTGCAGCACACTTAGCCAGACACACAGAGG10.641025641025641No Hit
GCCGAGGGAAGAGCAAGTCAGTGGAGCTGGAGGATGTGAAATTCCACCAGT10.641025641025641No Hit
TAGTGATGTTGCACACACACAGCTGAGATTTACTGAGAGGGGGTAGCAGTA10.641025641025641No Hit
ATACTCATCAATGGGCTCAATCATATAAATTACTTCTAAGCCATTGCTTTC10.641025641025641No Hit
ACCCTGGGTGACCCAGTACACAGAGTTCCTGTAGATGGGGCCCCAAGGATA10.641025641025641No Hit
AGGTACCTCAGAGCAAAAGTTAGGTGAACAGGCTCATTCATCTCCTGTCTC10.641025641025641No Hit
ATAATGAATCAAGTGTTTGAGGTACTAATGCTCTTTTGCAGATATGCAAGA10.641025641025641No Hit
CTACCTAGCATATTTCTGACAGTCAGGTGGATTATCTTGAAATTTTAGACA10.641025641025641No Hit
GTTACGATAACATTGTAAATTTGGTCATCTGCTAAAAGTGCACCTGGTTGA10.641025641025641No Hit
TCCTTGATTAGATGACAGCTGTGAGTGAGATATCTGCTGCTAACTCAGCTG10.641025641025641No Hit
GTACAAGACACATTTTTAAGTCCTCAAGGAAAATACAAGTGTTCCTCAAGT10.641025641025641No Hit
CTTCTATCTCTTATATTCTGTTGGTGATGTTTGCATCAATGACTCCTGATC10.641025641025641No Hit
TCTTTGAATGACACCAAAAACTGATCAGAATGAGTCCTATAAAGTATGCCC10.641025641025641No Hit
GCAGAAGCGCCCCAGAAGCCGTACCCTGACAGCAGTGCACGACGCCATCCT10.641025641025641No Hit
ACTCAAAACTGGCCATGGATGTGATATCCTAGGCAGGTAGAGATGGATGGA10.641025641025641No Hit
CTAACCCTCAATTCCTCCTTATAGCAAAAGTGAAATAACTGCCACAATGTA10.641025641025641No Hit
CTACAGGCGACAGCGCTGCAGACGCCATTATCCTCTGCTTCTCCGCTGCAC10.641025641025641No Hit
ACTCAGTTGCCTTGGAAGCAGCCTGGGATGTGGACGAGGAGTGTCCTGTCT10.641025641025641No Hit
TGTAGGAACCCTAAACCTCATAATTTTATCATTCACAACACACACCTTAGA10.641025641025641No Hit
GGTATGGACCCAGAAATGGAAGACCCCAACCGCCTCCCCCCAGACCGCGAA10.641025641025641No Hit
GGTATCAACGCAGAGTACGGGGGTGTCCGGGAGAGGCTGTCTCTTATACAC10.641025641025641No Hit
CATTAGATGTATATATGGAAGCCTCAAACAATGATTACAGGGTCTGTCTCT10.641025641025641No Hit
CTTCTCAACTTTACTGGGGTTTTTCCCATCTATTATTCACCGCATTACACC10.641025641025641No Hit
ACAAAAACGTGATGATTATGTGAGGTAATGCATACGTTAATTAGCTACATT10.641025641025641No Hit
GTAACAAATTTCTCCACTGAGGAGGTTGATTTAATTGCTCCAGAAAGACCG10.641025641025641No Hit
ATACCACTGCCCGTACTCTGAGTTGATACCTGTCTCTTATACACATCTCCG10.641025641025641No Hit
AATAACAACTGTACATCAGTCGCTCCAGCAGCTTGCCCAACATGCCCTTCC10.641025641025641No Hit
CTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCTTCTGCTTGAA10.641025641025641RNA PCR Primer, Index 27 (100% over 28bp)
AGGTTTGAGTAAGAGCTGAACATCCGCCGAGAGCTAAACGAGATGGAGCGA10.641025641025641No Hit
GAAGACGACGACGTCGCTGGTGCCTGAGAAGTCGTGCCGCACGTCGTCGCT10.641025641025641No Hit
GGCACATACATCTTGACCGCGGCGGGTGAACATCTTGACCGCGGTGGCTCG10.641025641025641No Hit
CTTGTCCTGTTCGACAAAGCGACATACGACAAGCTCTGTAAGGAGGTTCCG10.641025641025641No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT10.641025641025641Illumina Paired End PCR Primer 2 (95% over 21bp)
GTGAGGTGGTTGGTAGTGTCTGTCACAGGTATGTAGCAGAAGCTCTCAGAT10.641025641025641No Hit
ATTATGTTAAGGCTGTGCAGTCTACAATCAATCATGTAAAAGAAGAACGTC10.641025641025641No Hit
CCTGGAATCCCTGCATTCAAGAGTGAGACAAGAGGAGAAAAAGAGGCAAAG10.641025641025641No Hit
ATAATATCTTCAGCAGTTTCAGAAAGACTAGTGATATCAAACCGAGACTCA10.641025641025641No Hit
ATGGCCAGGTTGTCAGCCACCTTGTCCAGAAGGGCGGTTGTGGGCTTCTCC10.641025641025641No Hit
GGCTTGCGCAGCTGCTTCTTCTTGAAGTAAGCGTCAGTCCTGTCTCTTATA10.641025641025641No Hit
CCTAAGGAAGTGTGTACCCATCAACAGTGCCCAAGAAGGGCCACAGATTAT10.641025641025641No Hit
ATAATGAGGAAGATCAATAGAGTCCAAATTATAGGTGCTATCTGGATTTTC10.641025641025641No Hit
ACCCGCGAGCATGCTCTTCTGGCTTACACCCTGGGTGTGAAACAGCTGATT10.641025641025641No Hit
GGCTCTATGTACAGCCCAGGATACCTGTTCTTGTAGAATGAGCCCATGAAA10.641025641025641No Hit
GCTCCAAAGTACTCAGCTTCAGACATCCACTTCTGACAGCTTTACTTTCCC10.641025641025641No Hit
GTACATGTAGCACACCACTATTTTACTAATGTAAAAACCTTGTAAGTGATT10.641025641025641No Hit
GATGTGTTATGGTTCCAAAACATTGGTTTCATCCTATTAGTTCTGTTCCTT10.641025641025641No Hit
GTGTATTGTCCCCATACTGATGGACGTGGAACCCATGCTGGCCTTCAGTTA10.641025641025641No Hit
GGTGTGGCCTGACGAGGCAAAGTGTACATGGCTCCCAAAAACTGGTCGGCA10.641025641025641No Hit
GTTTAAGTCCTGCAAGTCGGGGCATCTTGGGGTTTTATTATTGTAAAGTGA10.641025641025641No Hit
GCCTCCGTACACCAATAAGTCTTTTCTGTAAAATGCTGAAAGAAGCTCATC10.641025641025641No Hit
GAACACAGACTGCCTCCACTCTGGTCTTTTTTTTCCTTCCTTGTTCTGTTT10.641025641025641No Hit
CACAGGAGCCCAGCATGAACACGATGTACACGACCTGTCTCTTATACACAT10.641025641025641No Hit
CACACACACACACACACAAATACATATTTTATACATTTCAAGTAAGTATTA10.641025641025641No Hit
CAAATAGTATATGCAAAAGCAAGACCACAATTTCCCAAAATAAAGAAGCCA10.641025641025641No Hit
TGGCAGCACATGGGACTGGAGAGGTAGCTAAGAGTTCTACATCTTGATCTG10.641025641025641No Hit
GCGTCATAGCAACCATAGCCTTCCTCATGATTAACGCCTGTCTCTTATACA10.641025641025641No Hit
ATTTGAGGGTTCGCGTGTGGCTGGCCACGAGTCTGTGGCTGCTCGTCTCCT10.641025641025641No Hit
GTGTAAAGCTTTTCTGTTATCTGAGTCAGTATATAATCTGTCTCTTATACA10.641025641025641No Hit
TTATAGTACGGCTGTGGATCCGTTCGTAGTTGGAGTTTGCTAGGCAGAATA10.641025641025641No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.641025641025641No Hit
GTAGAGAGGACAGGCGAATCAGTAGAAGCTCAGAGCCATCGCACAAACGAG10.641025641025641No Hit
GCCTGGAACATGGCATCCAGCCTGATGGCCAGATGCCAAGTGACAAGACCA10.641025641025641No Hit
TCTTAAGGAGAAAGCAATTTTGCAGGCAACAATGAGAGAAGAAAAGAAACT10.641025641025641No Hit
CACAAGTACTTGTTGAGTAAGAGGGAAGCCGGGTCCATCACGCCAGGTCAC10.641025641025641No Hit
GTATATAATCTACCCTTAACAGTGATAAAATGATTTCTGAGAACGTGGTTC10.641025641025641No Hit
TTTCACTTCTATAGGAGCAGATGCATTCGTAAATGCAGTCTTAATAAACCA10.641025641025641No Hit
ATGATGGCCTGGACGGCCTCTCAGAATCCCAAGACTCAAAGCTCCTTGATT10.641025641025641No Hit
CTTTAGAGGCAGAGACTCAACACAAGTTTGAAATGTCAGACTCATTGAAAG10.641025641025641No Hit
GTACTGAATCGTCGGATGGTCCATAACAAGGAACGTGCTTTGCTATTCATT10.641025641025641No Hit
GCTTCATACAGACCCCTGTGTATGGGGCGTGAAAGATGATACCCGTGTGTC10.641025641025641No Hit
GTACAGCTGATTGAACCTGTTCATGCATCTTCGCCAGCAGCTGGAGCGTCC10.641025641025641No Hit
TTGTTTGGCATCTTAAAACTTGGAGTTTGGGCATGTTCTGATGTAGTAGTA10.641025641025641No Hit
TCCGGATAGTCCATGTCAAACAGTGATTGTGTCTCATTTTCCACATTTTTC10.641025641025641No Hit
TCTTGTTTGGAAGTATGTCTGTTGGGATCCCTTTCCCGTATTTTAATTTGG10.641025641025641No Hit
CCTTTAGTCCCAGTACTTGGGAGGCAGAGGCAGGCGAATTTCTGAGTTCAA10.641025641025641No Hit
GTGTGTGTGTGTGTGTGTCTGTCTGTCTGTCTGTTTTTTGCTGGTGTCTCT10.641025641025641No Hit
GTGCTACTTGTGTGTTCAGAAATTGTTTCCTCCTTCAAGCTATTTGCAGAA10.641025641025641No Hit
CCCTTGGCGAGTCCCGCTCTGAGCGCTAGAACCCGGCTCCGGCCGGCCGTG10.641025641025641No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAACTGTCTCTTATACACATCTCC10.641025641025641No Hit
GGAAGAGGCACTGTTGCAGCTGCTGCCGCTGCAGCCACAGCCAGTATTGCA10.641025641025641No Hit
GTACCAAAGTGATCCTTCACCTCAAAGAAGACCAGACAGAGTACTTGGAGG10.641025641025641No Hit
CCGCAGGAGGACCAATACCCACCCAACATCGCTGTGAAGGTCAACCACAGC10.641025641025641No Hit
GGATATAACCCTGGACCAATAAAACAATGGAGGTGTTAAAATACTGGCCTA10.641025641025641No Hit
CTCTATAACCGCATCGGAGACATCGGATTCATTTTAGCTATAGTTTGATTT10.641025641025641No Hit
GCTACCAACTCTCCATTTTTTTTTTTTTATCATTTTGGGAAAAATCTATAA10.641025641025641No Hit
GGCTACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGA10.641025641025641No Hit
TTTCCAAAGAGATAATACAAAAGCAACAGACTTGGTTTCAAACACAAACAG10.641025641025641No Hit
GGGTAAGCTGAAGTGGAACCTGGCCGCAGTCACCCCTCACGACTTCATTGA10.641025641025641No Hit
AGAGAGATACTGCACAACTTAAATCTCAGGTTATGAGAGGGGTCTTGTAGA10.641025641025641No Hit
ACAGTACATTACATATGGCTCTTGTACAGAGTAAAACAGACCCCAGGAGTC10.641025641025641No Hit
GCCCCTCTTTGTGAAAATATGCCTAGCGGCAAGGCCAACAAGCCAGGGGAT10.641025641025641No Hit
CTCTACCAGTGAGTTTCATCAGCTAACATAAGGGGCTGTCTCTTATACACA10.641025641025641No Hit
CCATGAGGTTGCTGGGATTTGAACTCAGGACCTTCGGAAGCTGTCTCTTAT10.641025641025641No Hit
GTTCGAGGCTTGGCTGGGGATGTGAGCCAGCTACCCATCCCAGCCTCTCCT10.641025641025641No Hit
GTGAAAAGGAACAAAAGGGCTTCTAACACTAGAAAAAATTTAAGGCCAAAC10.641025641025641No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC10.641025641025641RNA PCR Primer, Index 46 (95% over 21bp)
GCCTGGAAGAAGGAGGTCTTCTCGGGCCCCAGACCAGTGTTCTGAGCTGGC10.641025641025641No Hit
GTACTATCCTTTGGAATCTCACTGAAGATGAGAAGCAGACAATTTCAGTGT10.641025641025641No Hit
CCCTTAATTGTGCCCACGTGCAGTCAAGAATTGGAAAGAATGAACAAAGTT10.641025641025641No Hit
CGCCTGGCCTCAGCTACCAGACGCACTGAGGCCTTGGAGCGGGAGCTGGAA10.641025641025641No Hit
GGCTTGCTAGCAGTGAGCGTTTCTTACTTTCTAAATCATCTTGCCTGCCCT10.641025641025641No Hit
GCCCAGTAACATTAGAAGTTGTCACATAAAAATTCAAGAACTTTTCTAGAA10.641025641025641No Hit
GTCTGGTGTCAGTACCCACTTTTCTCTCACTCCAGACCAGTTCAGCAAATA10.641025641025641No Hit
GCATGACCCACATCGTCCGGGAAGTTGACAGGCCAGGATCTAAGGTGAACA10.641025641025641No Hit
CTCTAAGAAACATGGTGGCCCGGCGGATGAAGAGAGGCATGTTGGAGACCT10.641025641025641No Hit
GCTGTGCATCAGCCCATGGCTGCCTACTCCTACCGCCCAGGCCCGGGTGGT10.641025641025641No Hit
AACTGCACCACAATATCAGCAGGATATGGCTTTGTTGTTCGTTCAGGAATC10.641025641025641No Hit
TCTTAAAGTGGGCTTTAGTCCAGAAGTCAGAAGCAAGCTGACCTTGCTCCG10.641025641025641No Hit
ATCTTACTTTGTAAATTCTGATTTATTTTCACCATTCGTTATGTAATGCTG10.641025641025641No Hit
TTCTTGGCTTATAAGAAGCTGTAACTGCCAGAGCCACAGTGAATGTGTCAC10.641025641025641No Hit
CAGGGTTTCTGTGTGTAGCCCTGGCTGTCCTGGAACTCTGTAGACCAGACT10.641025641025641No Hit
GCCGCGACTTCCTCAAAGGTGACTGTCAGAGAGGAGCCAAGTGCAAGTTTC10.641025641025641No Hit
CTGCTCGCTTTCGAGCGCCCCTCAGCAACTTCCGGGATAGAGGCGGCGAGG10.641025641025641No Hit
CTTCCTGCTTGTCAGTCTTTGTCACACTTATGATTGAGCACTGCACATCCT10.641025641025641No Hit
TGATAGTAGAGTTGAGTAGCGGGTAAATTTGAATTAAAATTGATAGGGGAG10.641025641025641No Hit
TGTGTATGGTGTTTGCTTTGTGGACACTTCACTGGGCAAGTTTTTTCATCG10.641025641025641No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers