FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_31.34100000007081.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_31.34100000007081.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432123
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC12440.2878809968458055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1800.036.24962643
TGCGGCG250.00210576335.9996310
CTCGATA250.00210576335.999632
ACGAGAC1900.035.52594822
CACGAGA1850.035.26990521
TCTCGTA1750.034.7139338
CGTATGC1850.034.05370341
CTCGTAT1800.033.74965339
CCACGAG1950.033.46119720
AATCTCG1850.032.83749836
GCCCACG2000.032.62466418
CCGGGGT352.8176536E-432.14252538
CGAGACC2100.032.14252523
TCTCCGA2150.031.39502511
ATCTCCG2100.031.0711110
GTATGCC2050.030.73139242
CCCACGA2150.030.34852419
TCGTATG2250.029.99969340
TTAGACC300.005144004729.999693
ATGCCGT2150.029.30202544
Produced by FastQC (version 0.11.2)