FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_30.34100000007074.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_30.34100000007074.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC2111.666666666666666No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC95.0No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT42.2222222222222223Illumina PCR Primer Index 8 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCTTC21.1111111111111112Illumina PCR Primer Index 8 (95% over 23bp)
CCTGAAGGGCTGCCCTGGCCAAGGAACCGCGAATCTTCAGTCTCTCAGAGA10.5555555555555556No Hit
CTCCAATACCCTGAATGAGTGTCACCTCCACTCCACCTCAGCCACTTCACT10.5555555555555556No Hit
AAGATTAAGTGTGCAACATGACATACTATGGAGACTCGGTGCTATGCCTGT10.5555555555555556No Hit
TGTTACATGGGTAAAATATTCCTTTATCATGTACAGAACAAGCTGAAACAG10.5555555555555556No Hit
CCTCACGCATGTGTACCCCATCCGTCTGGTCAAGGTCAATGAGGACACGAT10.5555555555555556No Hit
GTCTTGGCCCTCCTGAGGCCAGTGCTAGGAGCTCTGGATCAGGCGTCTATA10.5555555555555556No Hit
ATGCAGAGCTTCTAGACTTTTATAGCCTAGACTGCTACCTTTCAAAGCCTG10.5555555555555556No Hit
AGTCAACATAAGACATGTAAGAGTTAAGGGTGTGGTGACACAGGCCTTTAA10.5555555555555556No Hit
GAGAAGGAATAAAAAAACAGGGGAAGAAAGAAAGTAAAAAGGAAGGAAGGA10.5555555555555556No Hit
TTGTAAAGTGGTAACAGTCCAAAGAATCACCTAGCTAATTTCCATAAGAAT10.5555555555555556No Hit
CTTTGAATGTTCTTGAACTTAGCCAATTCTCTCCTGTAATACTCTCTATAC10.5555555555555556No Hit
GGTCTGAAAAGTAGAACGCGGATTTGGCTAGGGCCTCGCGTCTCCGCCGCC10.5555555555555556No Hit
ATCTTGGCTATGTGCCTCCCTTGCCCCTCCCAACACCCTGTCTACACACGT10.5555555555555556No Hit
GCGTATGGAAATCCATCTACACTTCCTTCAGTTCTTCTCACCAGCACTAAA10.5555555555555556No Hit
CCTCAGGCCCTCTCCTCTGGCTCCCTCTCCAGCAGCAGTTTTCTCAGCTGG10.5555555555555556No Hit
TCCTAATATAACAAGAGGATTGATGAAACAAATAAGTAGTAGTACTGTGTA10.5555555555555556No Hit
CTCAAATACCTTAATCTTTATGATAGACACATAAGTTACCCAGCACCTATT10.5555555555555556No Hit
TGAGTAGAGAGACCCACCTACTGACTGATGAACTGCGCTGACCCTGGGGTC10.5555555555555556No Hit
GTGTATAAGCTGCTCATTCTGTACAGTGAATTTTCTGAACCTTTCAAAGTA10.5555555555555556No Hit
GTCCCTCCACCACAATGTTCTGCCCAAAGGAATGGAGGCAACAGACCATGG10.5555555555555556No Hit
AGCTTTCTCTGTTCCTAATGATTTTCTTCACTTCTACAATAAGCTCCTTAG10.5555555555555556No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAG10.5555555555555556No Hit
CTGCTGGAAGAGTCAGAAGACCCAGCAGCACAGGGGCTTTTAGAGCACAAA10.5555555555555556No Hit
TCACATATTAGGTCAAATAGAAAACATCAGCAAACCTCATAAAACAGAAAT10.5555555555555556No Hit
CTACATGAGTGGACAGTTACAACGACAATCATCTTCTGTAATGACCATTTT10.5555555555555556No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTG10.5555555555555556No Hit
ATCAGACGCTGCCAGCTGACCCCCAATGACAGGTTCATTTTGCTGGCTTGC10.5555555555555556No Hit
GGCTAGACCAAGGAATATGCAGAAAAAGACTTGCCATCAGAGACAGCAGAG10.5555555555555556No Hit
CCTACAAGGATCATAGCGGACTACCACAACCTTCTCCTCAAACGCTGTCTC10.5555555555555556No Hit
GTCCACAAACATGGGGAATATCTTTGCAAACCTCTTCAAGGGCCTTTTTGG10.5555555555555556No Hit
GGCCTAGAGATATAGCTCAGTGGTAGAGTACTTGCATGTATGTACAAGACC10.5555555555555556No Hit
TCAGAGAGCAAGGCACCAGGGGCAAGAATCGTGCAGAATTTCAGAAGTCTG10.5555555555555556No Hit
GTTAAATACACTATTGGATTTTTTTTATTTTCTGTTTTGATTTCAGCTTAT10.5555555555555556No Hit
AAACAGAGTATAACTCCGAAGTCAGTGGTCAGTGAACACCTTACCAGACCA10.5555555555555556No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT10.5555555555555556RNA PCR Primer, Index 44 (95% over 22bp)
ATGCAGGCTCCATCAGGCACCGTGTCCCCAGGGGTGGGCATGAACCAGTGC10.5555555555555556No Hit
GGCTGAGAAACACTTCAAACAAACAAACAAACTAACTAACAACCCTTTTCC10.5555555555555556No Hit
TTTCCTCGCCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGA10.5555555555555556No Hit
GCCCAGCTCTCAATGGAGGTGCAGAATCATGGAAGATGGGCAAAACTTTCC10.5555555555555556No Hit
CAGCTGTACAGCGCCCGCCAGAGGAGGCGCCTGAACCGTGGTCTTCGGCGG10.5555555555555556No Hit
GTATGAAGCTTGAGATTTGGGATTTGCATCATAAAATGAATATTTAAAAAA10.5555555555555556No Hit
ATTTATGGTTGCTTGGGTGGAGAAGCCATTGTCTTCAAAAACCTTGATGTC10.5555555555555556No Hit
AGCTACTATTTATCAAATGCTTAGTTGTTCATTTCATGGGCTTCTATGGAG10.5555555555555556No Hit
CTTTACATCATTGTATGTGATCACTTGTCAGTGTTCAGGTGTATTTTAGCA10.5555555555555556No Hit
CTGTGTAGCTATGGCTGTCCTAGAACTCAATGATCTACTTAACTCCTGTCT10.5555555555555556No Hit
CTTATACACATTCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT10.5555555555555556No Hit
GTCTTCTGGAGATACCTGTTCGTGGTCCAGCATGGTGAGCCCTTTGACTCC10.5555555555555556No Hit
CTACAAGGCATACTTTCTCGAGGGTTTGTTTGTTTGTTTGTTTGTTTGTTT10.5555555555555556No Hit
ATTCTAGGCAAGGGAGGAAAGCTGTGGATGGAAGCAGAGCTGTCTGGGAAG10.5555555555555556No Hit
GATAAGGAGGTGGCTGGTGGAAACATTACCAATCTCATCCCGGTAATAGAC10.5555555555555556No Hit
CTTATACACATCTCCGAGCCCTCGAGACAACATAATATCTCGTATGCCGTC10.5555555555555556RNA PCR Primer, Index 44 (95% over 21bp)
CATAAATACAGTGGTATATTTACAGAGTGTTGTACATGTCCCCTTTGGAAA10.5555555555555556No Hit
GCTCAAAATTAAAATCAGGTGCTCCTGACTTGTCCCCCTCTTGACTAACCA10.5555555555555556No Hit
CTCTGAAGCTTTAAAACAAGCGAAGCAGACCCCTTTCAGCTCCCGAGCCTT10.5555555555555556No Hit
TAGTGATTCTCGGGGCTACTGGGACTGGCAAGTCCACCCTGGCTCTGCAGT10.5555555555555556No Hit
AGTTATGTGTTTTATGCATACACCACATAGACTGTCTCTTATACACATCTC10.5555555555555556No Hit
GGTCAGAAAAGGGCTGTCTTAGTCAGGGTTTCTATTCCTGGACAAAACATC10.5555555555555556No Hit
CTTATACACATCTTCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC10.5555555555555556RNA PCR Primer, Index 44 (95% over 21bp)
GCACAGCAAATGGTTCTTTTGTGCCTACAACACAGGGAAAGCCTAACTCAA10.5555555555555556No Hit
CTCTAGTACTAAAATATTGCCCCCAAGGGCTGGAGAGGTAGCTCACTGGTT10.5555555555555556No Hit
CAGGATGCCAAACTGCACAGGGCTCCAACCTCCCCAGAGTTGCCGGCTGGG10.5555555555555556No Hit
GAGAAGTCATAGATAAAAGATTTTTCATGTACATTTGGCAGGTTATGTCCT10.5555555555555556No Hit
GTCACCGACCTGTTGGTGGTCCATGAGCATCGAGGCACACCCGTGGGGCTC10.5555555555555556No Hit
GATCTGTATTGTTCTTACTGAAACACAGCATGGAATTAACACTGTCTCTTA10.5555555555555556No Hit
CTCCTGAGAGGCTCTGTCAGAGCCTGACAACAGAGATGAGCCAACCATTGG10.5555555555555556No Hit
CATAAGAACTAAACAGAAGCATTCTCGGAAACTACTTTGTGATGTTTGTAC10.5555555555555556No Hit
GGCGGACCACAAGGTCAGGAGTTCAAGACCAACCTGGCCAACATAGTGAAT10.5555555555555556No Hit
CTACAGAGTTGTTTGGTAGGAAGAGAAGGGGCTTCGCTGTATTCTCTTTGC10.5555555555555556No Hit
CTTCAAACACACCAGAAAGAGGGCATCGGATCCCATTACAGATGGTTGTGA10.5555555555555556No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGACGTCTTC10.5555555555555556No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC10.5555555555555556No Hit
ATGGCAACTTCCTCGTGTATATTTCCCTGAAAAGTGACATTATATCTGTTT10.5555555555555556No Hit
CTTCATACCTGTCCTGCCCTTCATTCTTGAGATTTTCCAGCAGGTGGACTT10.5555555555555556No Hit
GTTACGAACTGCCTGGTCTGTGATTCCCGAAGGGTCACATCCCATGGGCTG10.5555555555555556No Hit
GCCCTCTGTCGCAGTGCGAATACGAATAACTTGACGGATTTCATCTTCAAC10.5555555555555556No Hit
CCTCACCACCATCCTTCTAATTGCTTCAGATACCTTTTATTTCCTGGGGAC10.5555555555555556No Hit
GGGCATAGTCTGCTCTCGGGACATTCCATCCCTTGGATTACTTGATGACGT10.5555555555555556No Hit
GAAGCCCTGGAGGCTGCTTATATTTGTGCCGACACGTACCTGGTGAAAAGC10.5555555555555556No Hit
CTTTGTGGCAGACACAAAGAACTAAATATTGGTTTGCTATACCAAGAGTTT10.5555555555555556No Hit
CTTCTGGGGTCTGAATGGATCTTCTGATAATTGAGTAAGGATGGCGGGGGC10.5555555555555556No Hit
CCCAGAGACTATATGAGGGTTGTACTGTCATTCATACGATCCTGTGCCCTC10.5555555555555556No Hit
GACTGAATGGCTGGATGGCAAGCATGTGGACTTTGGGAAGGTGAAAGAAGG10.5555555555555556No Hit
GAGTCTTGCATCTCTCTACTGAAGTTTTCCAGGCAATTTTAACGGAATTAA10.5555555555555556No Hit
TCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCTTCTGCTTGA10.5555555555555556Illumina PCR Primer Index 8 (96% over 29bp)
ATCTAAAATTGACACCCTAACATCACAATTAAAAGAGCTAGAAAAGCAAGA10.5555555555555556No Hit
CTACAGGAGGCCCTATTACTTCCGTGGGCGAAACCGAGGCTTTTATCCATG10.5555555555555556No Hit
CTACTAGACAGCTTTTGCTGTGAGCTTAAAACTATTGCTCTCATGTGAGAC10.5555555555555556No Hit
GACCGGAACATGGCCAGGTCTTCGAGGGCCATCTGAGAGGGCCCGTCTTCC10.5555555555555556No Hit
GGATAACTCAAGGCACATTGATCGTGAGGAAGGGTGACTGTACACTAAGTG10.5555555555555556No Hit
GCCACATGCAGAGATGCCGATCACTAAAGTTCCATCTGCAAAAACAAATGG10.5555555555555556No Hit
GTCCTGGAATCACTATGTAGACCAGGCTGACCTGGAATTTATAGGAATCCT10.5555555555555556No Hit
GTGCTCATGGTGCAGAGGAAGAAAGAGCAAGTTGTATCACAGCCTAGCTAC10.5555555555555556No Hit
AACGAGTACATCATGAGTTTAATCAGTGACAACGCAGCGAAGATTCTGCCC10.5555555555555556No Hit
AAGTAAGACATTTCATGGGTGGCATTAGTTTTACTACGAGTGTTGTACTTT10.5555555555555556No Hit
CTGTATATCCCTGGCTGTCCTGAAACTCACTTTGTAGACCAGGCTGGCCTC10.5555555555555556No Hit
TTTCCAACCTTACTATCTGTTCTGATTTATCCCTTTTTAGGGGGTGATCAT10.5555555555555556No Hit
TGGTGCCTTTGGGAAGCCCCAGGGCACAGTGGCCAGGGTTCACATTGGCCA10.5555555555555556No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGCCGGAATCTCGTATGCCGTC10.5555555555555556No Hit
GGTAGAGCTCTAGCATATGCAGATCAATGATCAGTCAAATCATCTTCTCCA10.5555555555555556No Hit
AGGATCTACACAGTACCCATAGGCAGTGTCCTAGAGAAACAGCCTGTCTCT10.5555555555555556No Hit
ACAGATGGGAATGAAGCTTATGTATCCATTATCTGTCTCTTATACACATAT10.5555555555555556No Hit
GACTCCTGGTAGGGAAGAAGACTACTGCAAAAGGCAGTCAGGGCAGGAGAG10.5555555555555556No Hit
CATTTTAACAACATGCTATTCTTCTGCAGGTAAAGGAGACATGGCTATATC10.5555555555555556No Hit
GTTCCTAATGATTTTCTTCACTTCTACAATAAGCTCCTTAGTCAGAAAATG10.5555555555555556No Hit
CACCAAAAACTCCTATTCCAATTGATACTGATAACCCTTGTAAAAGGAAAT10.5555555555555556No Hit
TTTCTACCTAGATTCTTTGACATCTCCAGAGATGGAGATTTCTCCTTGTTT10.5555555555555556No Hit
TGGATAGAGAAAGAACCCCATGAAAGTGCCCTTCTCCAGTTAGACCTATTC10.5555555555555556No Hit
AACCCACCCACTCCCGCTTCCCTGTCCTGGTATTCCCCTGCACTAGGGCAT10.5555555555555556No Hit
CCTTAAGTGTGTATATCTCATTTTCCGTGATTTTCAGTTTTCTCGCCATAT10.5555555555555556No Hit
CACGTAGGCGTCGAAGACGCTTGCTTCAGATATGTCCCTGACAGCAGCGGC10.5555555555555556No Hit
GACTATCCAAGCACAGGACAGGCACCTCTCTGGAGAAAAAAATCTCTGACA10.5555555555555556No Hit
CCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAA10.5555555555555556No Hit
ATTAAAGTCCTTATTGATGTCCTCTACCATCATCATGTGATATGTTTTTAA10.5555555555555556No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTA10.5555555555555556No Hit
CTTCTACTGTCCCTGTGGCTAGGCCTTTCTTGCCAAGTGCGCTGACACATA10.5555555555555556No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT10.5555555555555556No Hit
TTACATGGCTTCTGAACACCTAGTGCCAAGGCCTGTCTCTTATACACATCT10.5555555555555556No Hit
GTGCCATCTATGAGGGACAGCCGCTGACGTGTCCTCATTGGCAGTGTGAAC10.5555555555555556No Hit
CCGGCACATTACACAGCGACCATCCCCCACATCCATTCCTGGACCAGCCCT10.5555555555555556No Hit
CAAATATCATTGGCTCTGTTGTTGCTGAACTCACCAAATCTCTTTGACTAC10.5555555555555556No Hit
TTCATTATCTTGATTGTGTTGAGACTTCACAGCTGTCTCTTATACACATCT10.5555555555555556No Hit
GTCAGAACACCTTGCTTCATGGGAAAACCTTGCTTGTCATTCCCACCGCTG10.5555555555555556No Hit
GTGTGTCCCCTGCAACCCCACTCCCTGTAGCAATTTCTGAGAAAAGTGCCA10.5555555555555556No Hit
GGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCGAAGTTCAAATCCCAGC10.5555555555555556No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCCTGTC10.5555555555555556No Hit
GATGCAGGCTGGCCGGGTGGCCCAATAGGGAACCCTAGCTCCTCCAGAGGC10.5555555555555556No Hit
GCTCTCCTCCCCTGTCCCCACTGCAGAGATGGCACCGTGGAGTTCCCTTCA10.5555555555555556No Hit
GAGTAGCATCTGTGATGTCAAGGCCTGCCAGCCGGAAGTCAGTCATGTTCC10.5555555555555556No Hit
ACTCTGAAGCACAAACATGCCGGTCAGTTTTGTGTTTCTCGGCATTTCTGA10.5555555555555556No Hit
CCACGCCAGCGTCCACCGGTCACTGCCGCCGCCCACAGAGACGTCGCCCAC10.5555555555555556No Hit
TGATGATTCCATTCTGCCAGGCTCCCTGGACCTCCCTGAGCTTCAGCATGA10.5555555555555556No Hit
TCTTCAGGGTCTGAGGCTTGAGCCCGCCTAGAAGTTTCTCTCAGGAGCAGG10.5555555555555556No Hit
ATCAAGCCCAAGATGCCAAAAGGCCCCAAACTCAAGCGGCTGGCTTTCATC10.5555555555555556No Hit
GAGACTCTGGTGTGGGTTTTCACAAGATGATCCGTGAATTCCTGATAAGGA10.5555555555555556No Hit
CCAGACCCTCTTCGCCACTTTTCAGAGCCTCCTCCCCCTCACTGGGTTTCT10.5555555555555556No Hit
GAAGAGCTGTCTGCTCAGCGCCATCGGGCAGAGGATAATGTTTGGGAAGCT10.5555555555555556No Hit
TCTGAGTGCTGACATTAAAGGTGTGCACCTCCACTCCTGGTTCCTCTGGTT10.5555555555555556No Hit
TCCTGCCACTTGGTGGTAAATTTGGTTCCTTTTTTGTCATAGTAGTGGTCT10.5555555555555556No Hit
CTATAATACCATCCATATCCAAAAGTCGAGCAATATCCTGTCTCTTATACA10.5555555555555556No Hit
GAATCAAACACTGCTCCTCAGGAAGAGCAGCAAGCATTACCTACCTCTGAG10.5555555555555556No Hit
GCCAGGGCAGGAGCTCAAGGCAGGAATCTGGAGGCAGAATCCTTGGAGGAA10.5555555555555556No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATTCGTATGCCGTCT10.5555555555555556No Hit
GTGTGGTAGGCCCTAGGTGCCTGCCAGGCTCAGGCCTCAAGAGCTCTTGAG10.5555555555555556No Hit
GTAGTATACACCAGTCAGTAATCTCAGTACTTGCAATTAAATAGAGTCAGG10.5555555555555556No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers