FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_3.34100000006e6c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_3.34100000006e6c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356318
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC7290.20459252690012852RNA PCR Primer, Index 27 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG653.6561687E-1034.61387336
GAGACGA751.6934791E-930.00290124
GACGAAT602.409015E-730.002926
TATGCCG751.6952981E-929.9986943
CGTATGC803.3796823E-928.12377241
CGAGACG803.3796823E-928.12377223
ATGCCGT803.3796823E-928.12377244
ACGAGAC803.3796823E-928.12377222
CGAATTG654.8534093E-727.69498428
ACGAATT709.2695336E-725.7167727
CTCGTAT901.1857992E-824.99890939
TCGTATG901.1857992E-824.99890940
TGCCGTC952.1029336E-823.686545
ATCTCCG952.106026E-823.68317810
CACGAGA952.106026E-823.68317821
CGAGCCC952.106026E-823.68317815
CCACGAG1052.3537723E-923.570420
ATCTCGT1003.6281563E-822.49901837
TCTCGTA1003.6281563E-822.49901838
CCTATAC500.002262140622.4990183
Produced by FastQC (version 0.11.2)