FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_27.3410000000704e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_27.3410000000704e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426859
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC35420.8297821997427722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGT207.027396E-445.001178
TGCCGTC4450.033.8772945
TATGCCG4450.033.87331843
CCACGAG4650.032.90022720
CACGAGA4600.032.76875321
GCCCACG4750.032.21513718
ACGAGAC4750.032.20759222
AATCTCG4700.032.0715536
CGAGACC4500.031.99708223
CGAGCCC4800.031.87956215
TCTCCGA4800.031.87582811
ATCTCGT4800.031.87209537
CTCGTAT4800.031.87209539
TCGTATG4800.031.87209540
ATGCCGT4800.031.87209544
TCTCGTA4900.031.68078838
ATCTCCG4800.031.40706610
CGTATGC4900.031.22164541
CCCACGA4900.031.22164519
CTCCGAG4850.031.08692412
Produced by FastQC (version 0.11.2)