FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_26.34100000007031.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_26.34100000007031.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462209
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC29650.6414846963170341No Hit
GTAAATATACAGTTGGATGACATTCTGATACTACAAAGTTACTTTTCTGGC11590.25075236527198735No Hit
ATTTAAGACCTGCTAAGTGAGTGAACAAAATTTGCTAATATCAATTAATGA5550.12007555023809575No Hit
ATATACAGTTGGATGACATTCTGATACTACAAAGTTACTTTTCTGGCTCAT5290.11445038932604082No Hit
ATTTAACATTGTCCACTCATTTGCCATACTGACTGTGCAAATACAAATCTG4700.10168560110253154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC3600.040.62392422
TGCCGTC3700.039.5259845
CGAGACC3750.038.9989723
ATGCCGT3750.038.9989744
TCGTATG3950.038.73315440
CTCGTAT3900.038.65282439
CGTATGC3800.038.48582541
TATGCCG3800.038.48582543
TCTCGTA3900.037.4990138
CCTTGCA4050.036.665728
GTATGCC4050.036.11015742
AATCTCG4050.036.11015736
ATCTCGT4100.035.66978537
CACGAGA4100.035.66978521
ATCTCCG4200.034.82427610
CCACGAG4200.034.82050720
GAATCTC4300.034.5339735
CTCCGAG4250.034.41457712
GCCCACG4250.034.41085418
CCCACGA4250.034.41085419
Produced by FastQC (version 0.11.2)