FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_25.34100000007024.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_25.34100000007024.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451691
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC17510.3876543920512031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1800.039.99881743
TCTCGTA1800.039.99881738
CGTATGC1950.038.07579841
CTCGTAT1950.038.07579839
TGCCGTC1900.037.8936245
CCGAGCC2000.037.123914
CGAGCCC2050.036.21844515
AATCTCG2000.035.99893636
ATGCCGT2000.035.99893644
GAATCTC2050.035.12091435
TCGTATG2150.034.53386340
GCCCACG2250.033.99899718
TCCGAGC2200.033.74900413
CCCACGA2200.033.74900419
ACGAGAC2150.033.4873822
CGAGACC2100.033.21330623
ATCTCGT2200.032.72630737
ATCTCCG2300.032.2852310
TCTCCGA2300.032.2852311
ACCTTGC2250.030.99908427
Produced by FastQC (version 0.11.2)