FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_24.34100000007017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_24.34100000007017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384339
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC7330.19071704927160657No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGT207.0278207E-444.99947723
AAGCCGT352.8170992E-432.1424836
TAAGCCG300.005143335629.9996535
CGGAGAT406.1449833E-428.1356561
TAGTACC406.159034E-428.1246744
CCCACGA1004.5474735E-1126.9996919
CCACGAG1004.5474735E-1126.9996920
CTAGTAC608.173749E-626.2496973
TATCTCG1058.367351E-1125.7139936
ACTAAGC450.001225151724.999713
CGTATGC1101.5097612E-1024.5451741
CACGAGA1101.5097612E-1024.5451721
GCGTCAG1301.8189894E-1224.239951
TATGCCG1152.6375346E-1023.4779943
CGAGCCC1152.6375346E-1023.4779915
CGTCAGA1353.6379788E-1223.3330632
CGAGAAA602.4635575E-422.4997421
ATCTCCG1257.4760464E-1021.59974910
GCCCACG1257.4760464E-1021.59974918
CGAGACT1257.4760464E-1021.59974923
Produced by FastQC (version 0.11.2)