FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_23.3410000000700a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_23.3410000000700a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793213
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1200.026.2495639
GTATCAA4350.025.3507941
TATGCCG1251.8189894E-1225.19957743
ACGAGAC1353.6379788E-1223.33294122
TATCTCG1459.094947E-1221.72377436
ACTAGAT1501.4551915E-1120.9996527
TCTCGTA1501.4551915E-1120.9996538
CACGAGA1552.3646862E-1120.32223921
CAACGCA4800.020.155915
GCCCACG1703.6379788E-1219.8538618
CGTATGC1703.6379788E-1219.8526141
TAGATCC1703.6379788E-1219.8526129
ATCTCCG1603.8198777E-1119.6871710
TCAACGC5000.019.3496764
TAACACC706.981648E-419.285394
CGAGACT1656.002665E-1119.0905923
ATGCCGT1656.002665E-1119.0905944
ATCAACG5150.018.7860933
AAATTCG600.006509390718.74968521
TAGGACG2050.018.6582224
Produced by FastQC (version 0.11.2)