FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_22.34100000006fff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_22.34100000006fff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC2317.557251908396946No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC118.396946564885496No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCTTC21.5267175572519083Illumina PCR Primer Index 8 (95% over 23bp)
CACAAGGAAGGCGTCCTCTCCTGATAGCTAACATAGGCCTGTGTGTTGCCT10.7633587786259541No Hit
CTTCTGAAACAGAAATGTTTGTAATTTTTATTTCTGTCATGGTATCTGGCC10.7633587786259541No Hit
ACTTATACATCTTCTATGAAAAAACTTTCTACCCCTAACACTAGCATTATG10.7633587786259541No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGGAATCTCGTATGCCGT10.7633587786259541No Hit
CGTCAGATGTGTATAAGAGACAGTATTCTACCCTCCTTAAATCTAGGCTAC10.7633587786259541No Hit
ACTGAATTCTTAAGTCAAAGGGAAAAGGATTATCCTTTGTTTTGGACTAAC10.7633587786259541No Hit
TCCTGTTCCTTATACTCTTAGCACCGAGAACCTTCTCTTTTAGTTCATATC10.7633587786259541No Hit
CTCCACAGGTCTTGCCTCAGTTGACATCATTCTGCCAATCAGCCCGAGTCC10.7633587786259541No Hit
GTATACGTCATTGTTCATAATCTGTAATCGCACAATGTCTGTCTCTTATCC10.7633587786259541No Hit
ATGCTTAACAGACCTTCCAAGTTTGACTGCATAATGACATCACGTCAAACT10.7633587786259541No Hit
AGCAAAAGAAATAACCTGCTATAATTGTGGACAAACAGGTCATATACAAAA10.7633587786259541No Hit
CAGTACTGTAGGGCCCCACATGGCCTCCATCTCAGACCCAACTGATGCGCT10.7633587786259541No Hit
CTTATAGATGGCTCCCTTTCAATGCCATGCTTCCTGCCCACTTCCCAGACA10.7633587786259541No Hit
CAATCGTAATCATCTGATCCAGGTGAGGAAGGGGGACGTGTGTAGATGCAA10.7633587786259541No Hit
CGTGGAACCCATGCTGGCCTTCAGTTAATCCTGTAATTTGTCCTGACAACA10.7633587786259541No Hit
CTCCAATAGCAGGGGCAGGTCGTGGGCGAGCAGGTCCCCTGCGGAACCTCG10.7633587786259541No Hit
GCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT10.7633587786259541No Hit
CCCCTGGGGACACGGTGCCTGATGGAGCCTGCATGGCTGGCTAGGTGCAGG10.7633587786259541No Hit
GGACATGGTTAGTGTATGTGACGCTCTGGATGCTGGGAGCGGAGATTTCAG10.7633587786259541No Hit
TTCCCCGGGGTGGACTCAGGGCATGGACGCGACCATCCTCCTCTTAGGAGC10.7633587786259541No Hit
CACTTATGTCACTCCCTTTGGCTAACAGGCTATTGCACCACTGTCTCTTAT10.7633587786259541No Hit
CTTATACACATCTCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT10.7633587786259541Illumina PCR Primer Index 8 (95% over 21bp)
GTATCAACGCAGAGTACGGGACTCGGTTGCTGACCCTTATACACATCTCCG10.7633587786259541No Hit
TCTGATTTCCGTTGCAAGTCCACACATTTAGCGACTGTAATGCTAAGTCCA10.7633587786259541No Hit
GGCCACTTGCTGTGTGATATCTGTCGTCCTCTCACAGCTGTCTCTTATACA10.7633587786259541No Hit
CTCTTGTATCTTCAGGTCAGAATGCAGCTCGGCCAGCCTCACAGGAGACAG10.7633587786259541No Hit
CACTAGCATTAAGTCTATGAGTTCCCCTACCAATACCACACCCATTAATTA10.7633587786259541No Hit
CTTATACACATCTCCGAGCCCACGAGTCTTGCTGGAATCTCGTATGCCGTC10.7633587786259541No Hit
CTACTGGGTTGGTGAAGATTCCACATATAAATTTTTTGAGGTTATCCTCAT10.7633587786259541No Hit
GTCCTGATCATCCTCACTGGGCGCCACAGGGGCAAGAGAGTGGTTTTCCTG10.7633587786259541No Hit
TCCCTGTGGTCTTCAGATACTCATCAAACACCAGTCGTAGGTGCTGTTTGC10.7633587786259541No Hit
GGCATATTTTAAGCAGTAAGCAGTCATCAATATAATGGTGGTAGCCTAAAT10.7633587786259541No Hit
CTTCAGCTACATTTTCAGCTTCGTGAGAAACCTCATCATCAAAGGAGATGG10.7633587786259541No Hit
CAAAACATACACTACACACACCACATTACACACAAACACACACACACACAC10.7633587786259541No Hit
TATGTAGACACCAGAGTCTCACTCACTATGTACTCCTGGCTGGCCTGGGAC10.7633587786259541No Hit
CCATTATAGCCATCCCCACTGCCACCATATCCACCACCACCACTGTCTCTT10.7633587786259541No Hit
GGTTAGTGGGACTGTGGGCTAAAACAGGACATTAAGTAAGGTGATTTGTCA10.7633587786259541No Hit
AACACAAACAGGTGAAGGAAAGGAACAAAACCATCCAGGATCTAAAAATGG10.7633587786259541No Hit
CTAGATAGCAAGTGATATGAAATCTAGAAGATGAAAACACTGACTAGTTTA10.7633587786259541No Hit
ACCACAGCGTTGGTAACAGTCTTTCCGAGGTACGCTTCTGCAATTTCCTTC10.7633587786259541No Hit
GTTCTGATCAGCTATGCTAACCTTTCTGAATGTACCCCAGTGGATGTGAAT10.7633587786259541No Hit
ACTCTACAATGGGCTACATGATGGCCAAAAAAACACCTGGAGATCAACCCT10.7633587786259541No Hit
CCTTATGTGTCTTTGTTAGAGTTCCTGTTGATATGGGAAAAAAATGAGACT10.7633587786259541No Hit
AGCCTGAAGAACAAAAGTCTCAGAAAGCAAAGAACAGCATCACCTTGATAA10.7633587786259541No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA10.7633587786259541No Hit
GTGCATGTACACCCCAGATTAGTCAGGCTCTTAAGGAGCATGGAGGGAACT10.7633587786259541No Hit
ACTTAACACCACTCACAGATTTAAGTCTGCAAAGGATCTCCTAGCACTACT10.7633587786259541No Hit
CTACTGAATCTCAAAATGTGCCTATTTCACTGGGCATGGGCTGAATGTCTG10.7633587786259541No Hit
CCCCTATACCTTACTCGCGGCCCCGCACCCGCTGCTCCTCTACCCACCAAC10.7633587786259541No Hit
TCTCCCGGGGAAGCATGGCGATGAAAGCGGTGTGCGTGCTGAAGGGCGACG10.7633587786259541No Hit
GTATAATGTTAGCTGTGGGTTTGTGGTTAATCTGTCTCTTATACACTCTCC10.7633587786259541No Hit
GTGCTATGCATGTGTAAGCTGAATAAACCTTTTCCTCCCCAACTTGGTCTT10.7633587786259541No Hit
CACACACACACACACACCCGTACTCTGCGTTGATACCTGTCTCTTATACAC10.7633587786259541No Hit
AATAAAGACATGAGAGAGGCAGAGAAGACTTTAACAGAACTCAACAAGTGT10.7633587786259541No Hit
AGCAAGCTGCAAGTGACGAGGGGTGATACGCTTTACCTTTAAGTCTTTTGA10.7633587786259541No Hit
GGCTACCATTTTCTCTCCCCTGCTCCCCTCACCAGGGGACAATTCCCTGGA10.7633587786259541No Hit
GTTCCACGTCCATCAGTATGGGGACAATACACAAGGCTGTACCAGTGCAGG10.7633587786259541No Hit
GACCTGTACCTGCGGATCCTCATGGCCATCGATTCAGAGCTGGTGGATCGG10.7633587786259541No Hit
ATTCTCAGCAATCAGACTGTGGACATTCTAGAGAATGTCGAAATCACTCTG10.7633587786259541No Hit
CTGGAACTCACATTGTAGACCAGGCTGGCCTCAAACTCAGAAATCCCCCTG10.7633587786259541No Hit
CGTGCTGGATTGTGTCCTCCTGGCTGGAGTGGGGAATAATTCCTGTCTCTT10.7633587786259541No Hit
TACATATACAGATAAGTAGGTAGGTAGGTAGATGGATGGAGAGAGAGAGAG10.7633587786259541No Hit
CATTTAGGTTGAGGTCAGTGAGTTTTTTTTTTTTTAACCTCTTTCCAAACC10.7633587786259541No Hit
CACTGAGGACGTTCAGGCAAAAACTGTCTGACTAACTTCCATGGTATGGAT10.7633587786259541No Hit
GGGTAATCAATTCTCCAGCATCGAATCGAGCCACCACCGACTTGAGGATCT10.7633587786259541No Hit
AGCTGCATGTCGGCCCCCAAGGTTCTCAGGTTCTAGTTTCTGGAAGGCATA10.7633587786259541No Hit
GATATATTCCATGTCCTACAGTGGACATTTCGAAATTTTCCAACTTTTTCA10.7633587786259541No Hit
ATGAATACCTTCAGTGTGGTGCCCTCGCCCAAAGTGTCTGATACCTGTCTC10.7633587786259541No Hit
CTCTGAGGCCCGTGAGGACATGGCTGCCCTAGAGAAGGATTATGAGGAGGT10.7633587786259541No Hit
GATTAATCCTTGGTGATTGTCTTCTTTACCAGTTTCTGGTAGTTCAGAATC10.7633587786259541No Hit
CTTAGCGTCCTTGAAGACACTTTCCAGGTCCGCGGGGCCGGCGTGGTCGCT10.7633587786259541No Hit
AGCATATATGTGGAAACTTAACGAGAAAGTAATGAGTCTTGGTGTTATTAC10.7633587786259541No Hit
ACGTAGAAGCGGACGCCGTGGTCAAGATGTCTCTGCCATCCCCACAGGACG10.7633587786259541No Hit
ATCACGGATAATGAGAAATACACACTTTAGGACGTGAAATATGGCGAGGAA10.7633587786259541No Hit
TTCTATGGCTCATTGAAGGTGACCAGGGAATGGGCTGTCTCTTATACACAT10.7633587786259541No Hit
CCCCTACTTAAAGGTCCCTCAGGATGTTTGCCTGTGAAGAAAACCAGCTCA10.7633587786259541No Hit
CTAGATGGTAGAGAGGTTGTGTCCCAGCTACAAGTTTTTGTTTTCAGAGGC10.7633587786259541No Hit
GAGGGAGGAAGTGTAAGAAGCAAAAGCCTCGTGCGCGCTACAGGCCATACA10.7633587786259541No Hit
GGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACAT10.7633587786259541No Hit
CTTGAGCAGAGGCCACGGCTAGTGTTTGTGACATGATGCAGCTATGGGTGG10.7633587786259541No Hit
ACCTGAATGTCACCAAAATAAAAATAGCAGATTACTTACAGGCTGTCTCTT10.7633587786259541No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT10.7633587786259541No Hit
AACCTGCTTGGAAGAACATAAGTAAGTCTCAGTAAACAATAATGCCTGTCT10.7633587786259541No Hit
CTCTGAACCCTGGGACGTGAATCTGCCACTTGAAACTTGGCGACAGTTTTC10.7633587786259541No Hit
GTCATTGGCAAAATAATCTATCTCAGTAACTATAGCAGTCTACCAGTAATG10.7633587786259541No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGAATCTCGTATGCCGTCT10.7633587786259541No Hit
ATTCTATACCTTTGCACCACTGGTGGCTGAAGAGGCCAATGCCCTGGTGTC10.7633587786259541No Hit
GCAACATCTAGCCTATCAGTTTACTCCATTCTATGATCAGGATGAGCCTCA10.7633587786259541No Hit
ATCCACAAGTCTCTGATTGGAAAGAAGGGGCAGCAGAAAACTGCTTAGGAC10.7633587786259541No Hit
ATCCAGCATTTAGTGACTTCCACTCTGACAAAGCTGGGAGGAGGGACATTA10.7633587786259541No Hit
GTGTCGACAGATTATTATCTCTGTAGATGACACTCACGCTGAATTCTGAAC10.7633587786259541No Hit
GGCCATTAGATTCCCTAGCAGTTGTGAGCCACCTAGTGTGGGGTGCTGGGA10.7633587786259541No Hit
CACCACCTTGGTGGAGAACAAGAAGGCTCAGCTGGTGGTGATTGCCCATGA10.7633587786259541No Hit
TCTCTGTGGACCCGAGGAGGCGAAACAAGTCCACGGAGTCACTGCAGGCCA10.7633587786259541No Hit
TCCTTGATGATTCTGTTGTTCTGGCTGCAACATAACTAAATCAAGTACAAA10.7633587786259541No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers