FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_20.34100000006fd5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_20.34100000006fd5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516596
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC15960.3089454815755445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1800.038.7496743
CTCGTAT1850.037.70237739
TATCTCG1900.035.5260136
CGAGACT1950.034.61508623
TGCCGTC2150.033.48808745
CACGAGA2100.033.21421
ATGCCGT2100.033.21444
CGTATGC2150.032.4415841
TCTCGTA2150.032.4415838
ACGAGAC2150.032.4415822
ATCTCCG2350.031.59547410
GCCCACG2250.030.99973518
CCCACGA2250.030.99973519
TCGTATG2250.030.99973540
CGAGCCC2250.030.99973515
TCTCCGA2350.029.68059711
TCCGAGC2450.029.387513
CCGAGCC2450.028.46914114
CTAGATC2250.027.9997628
ATCTCGT2500.027.8997637
Produced by FastQC (version 0.11.2)