FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_2.34100000006e43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_2.34100000006e43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309589
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC7650.24710180271262866RNA PCR Primer, Index 27 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1100.036.81778339
GAGACGA1100.036.81778324
TATGCCG1100.034.7723543
CGAATTG1100.034.7723528
GACGAAT1100.034.7723526
ATCTCCG1200.033.74963810
TATCTCG1200.033.74963836
CGAGACG1200.033.74963823
TCTCGTA1250.032.3996538
ACGAGAC1250.032.3996522
ACGAATT1250.032.3996527
ATGCCGT1200.031.87465744
CCGAGCC1350.031.66632714
CGAGCCC1350.031.66632715
GCTATCT1250.030.5996734
AGACGAA1250.030.5996725
TGCCGTC1250.030.5996745
GCCCACG1350.029.99967818
GAATTGC1350.029.99967829
CGTATGC1400.028.9282641
Produced by FastQC (version 0.11.2)