##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 12h_19.34100000006fc8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9808 Sequences flagged as poor quality 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.285889070146816 38.0 38.0 38.0 32.0 38.0 2 36.37612153344209 38.0 38.0 38.0 32.0 38.0 3 36.249184339314844 38.0 38.0 38.0 32.0 38.0 4 36.427508156606855 38.0 38.0 38.0 32.0 38.0 5 36.45789151712887 38.0 38.0 38.0 32.0 38.0 6 38.37357259380098 40.0 38.0 40.0 38.0 40.0 7 38.22777324632953 40.0 38.0 40.0 38.0 40.0 8 38.20942088091354 40.0 38.0 40.0 38.0 40.0 9 38.227671288743885 40.0 38.0 40.0 38.0 40.0 10 38.17139070146819 40.0 38.0 40.0 38.0 40.0 11 38.234502446982056 40.0 38.0 40.0 38.0 40.0 12 38.024877650897224 40.0 38.0 40.0 38.0 40.0 13 38.139172104404565 40.0 38.0 40.0 38.0 40.0 14 38.06647634584013 40.0 38.0 40.0 38.0 40.0 15 38.08034257748776 40.0 38.0 40.0 32.0 40.0 16 37.92526508972268 40.0 38.0 40.0 32.0 40.0 17 37.83309543230016 40.0 38.0 40.0 32.0 40.0 18 37.79588091353997 40.0 38.0 40.0 32.0 40.0 19 37.94127243066884 40.0 38.0 40.0 32.0 40.0 20 37.989702283849915 40.0 38.0 40.0 32.0 40.0 21 38.01610929853181 40.0 38.0 40.0 32.0 40.0 22 38.04057911908646 40.0 38.0 40.0 32.0 40.0 23 38.011623164763456 40.0 38.0 40.0 32.0 40.0 24 37.94963295269168 40.0 38.0 40.0 32.0 40.0 25 37.808931484502445 40.0 38.0 40.0 32.0 40.0 26 37.84094616639478 40.0 38.0 40.0 32.0 40.0 27 37.729098694942905 40.0 38.0 40.0 32.0 40.0 28 37.802100326264274 40.0 38.0 40.0 32.0 40.0 29 37.81973898858075 40.0 38.0 40.0 32.0 40.0 30 37.83584828711256 40.0 38.0 40.0 32.0 40.0 31 37.794147634584014 40.0 38.0 40.0 32.0 40.0 32 37.73480831973899 40.0 38.0 40.0 32.0 40.0 33 37.71890293637847 40.0 38.0 40.0 32.0 40.0 34 37.79608482871126 40.0 38.0 40.0 32.0 40.0 35 37.82748776508972 40.0 38.0 40.0 32.0 40.0 36 37.79730831973899 40.0 38.0 40.0 32.0 40.0 37 37.794351549755305 40.0 38.0 40.0 32.0 40.0 38 37.77028955954323 40.0 38.0 40.0 32.0 40.0 39 37.67791598694943 40.0 38.0 40.0 32.0 40.0 40 37.632442903752036 40.0 38.0 40.0 32.0 40.0 41 37.757340946166394 40.0 38.0 40.0 32.0 40.0 42 37.7925163132137 40.0 38.0 40.0 32.0 40.0 43 37.78395187601958 40.0 38.0 40.0 32.0 40.0 44 37.68209624796085 40.0 38.0 40.0 32.0 40.0 45 37.66211256117455 40.0 38.0 40.0 32.0 40.0 46 37.57575448613377 40.0 38.0 40.0 32.0 40.0 47 37.65089722675367 40.0 38.0 40.0 32.0 40.0 48 37.615008156606855 40.0 38.0 40.0 32.0 40.0 49 37.53211663947798 40.0 38.0 40.0 32.0 40.0 50 37.37897634584013 40.0 38.0 40.0 32.0 40.0 51 36.97971044045677 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 -0.549916074624015 2201 2 -1.5268634437619113 2201 3 0.9789164406714761 2201 4 0.9764125417200802 2201 5 1.0942886328444033 2201 6 1.2958907827504262 2201 7 1.1655751248557848 2201 8 0.8276787729223472 2201 9 1.2622274553147221 2201 10 1.073367250291966 2201 11 0.5991131559896203 2201 12 -0.1730884422514336 2201 13 0.438242816574018 2201 14 -1.040110100329244 2201 15 -1.4238854919267894 2201 16 -0.29624825001119603 2201 17 -1.5049673802800072 2201 18 -1.9655203741262142 2201 19 -2.148159636939752 2201 20 -0.05754700887186459 2201 21 -0.2370193685662798 2201 22 1.0450423059187344 2201 23 -1.533521072365886 2201 24 0.5114313511565811 2201 25 1.6928872674506863 2201 26 0.18010550029767103 2201 27 1.0936719294207649 2201 28 0.21511756405574545 2201 29 -1.3261391164224676 2201 30 -1.0851669271561022 2201 31 -2.35102335899127 2201 32 -0.044909750001799864 2201 33 0.8521192241925704 2201 34 -1.3037989389297877 2201 35 0.8630777895538131 2201 36 -0.30233100890875164 2201 37 -0.42920619857599007 2201 38 -0.5946047723373269 2201 39 -0.13121196607131225 2201 40 0.16060681758364836 2201 41 0.04853621046781598 2201 42 -0.6693430737487844 2201 43 -0.26877950078172574 2201 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-2.94315259755831 1213 43 -2.233065215067441 1213 44 -2.3718854155229465 1213 45 -2.7349465008272844 1213 46 -1.335531843981073 1213 47 -1.713778558768162 1213 48 -0.27011507698367154 1213 49 -1.1229466341997707 1213 50 -2.43605099542539 1213 51 -5.378826185760083 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 3.0 22 1.0 23 5.0 24 22.0 25 30.0 26 44.0 27 64.0 28 78.0 29 128.0 30 158.0 31 173.0 32 208.0 33 236.0 34 294.0 35 394.0 36 534.0 37 856.0 38 1585.0 39 4994.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.42017749668469 23.911047638478017 14.985208609609305 33.683566255227994 2 10.78711256117455 21.900489396411093 53.10970636215334 14.202691680261012 3 12.347063621533442 28.486949429037523 44.91231647634584 14.253670473083197 4 8.187194127243066 36.7862969004894 36.48042414355628 18.54608482871126 5 7.738580750407831 35.307911908646005 48.5216150081566 8.431892332789559 6 18.117862969004893 53.02814029363785 18.62765089722675 10.226345840130506 7 14.019168026101141 30.96451876019576 25.672920065252853 29.343393148450243 8 15.446574225122351 52.15130505709625 20.626019575856443 11.77610114192496 9 17.903752039151712 16.863784665579118 22.512234910277325 42.72022838499184 10 9.45146818923328 42.98531810766721 33.06484502446982 14.498368678629689 11 21.890293637846657 22.50203915171289 40.4873572593801 15.12030995106036 12 13.009787928221861 22.78752039151713 32.718189233278956 31.484502446982056 13 18.43393148450245 20.982871125611744 43.780587275693314 16.802610114192497 14 13.66231647634584 20.8605220228385 26.070554649265905 39.40660685154976 15 16.802610114192497 26.42740619902121 23.450244698205548 33.31973898858075 16 34.349510603588904 28.099510603588907 23.72553017944535 13.825448613376834 17 16.721044045676997 44.11704730831974 24.19453507340946 14.9673735725938 18 32.06893035586826 26.746201692668503 24.79861323544407 16.38625471601917 19 14.059951060358891 26.814845024469818 25.662724306688418 33.46247960848287 20 13.968189233278954 25.97879282218597 26.610929853181077 33.442088091353995 21 14.590130505709626 26.814845024469818 25.101957585644374 33.49306688417618 22 15.834013050570963 44.77977161500816 23.78670473083197 15.599510603588907 23 16.17047308319739 26.070554649265905 25.570962479608482 32.18800978792822 24 32.2389885807504 26.68230016313214 25.11215334420881 15.966557911908646 25 16.986133768352367 42.21044045676998 25.89722675367047 14.906199021207179 26 33.38091353996737 27.345024469820554 25.20391517128874 14.070146818923329 27 15.161092985318108 42.241027732463294 25.999184339314844 16.59869494290375 28 15.344616639477977 25.152936378466556 27.07993474714519 32.42251223491028 29 15.446574225122351 28.293230016313213 42.31239804241436 13.947797716150081 30 16.223105944733355 42.14336698276741 26.042622616498416 15.590904456000814 31 32.82014681892333 26.243882544861336 23.98042414355628 16.955546492659053 32 17.016721044045678 42.669249592169656 25.040783034257746 15.273246329526916 33 15.935970636215336 26.2336867862969 41.75163132137031 16.078711256117455 34 15.619902120717782 24.439233278955953 27.334828711256115 32.60603588907014 35 16.08890701468189 24.459624796084828 27.37561174551387 32.07585644371942 36 14.457585644371942 25.72389885807504 43.699021207177815 16.1194942903752 37 14.814437194127242 42.90375203915171 26.998368678629692 15.283442088091354 38 15.171288743882544 24.153752039151712 44.19861337683524 16.476345840130506 39 15.538336052202284 22.85889070146819 27.29404567699837 34.30872756933116 40 14.437194127243066 23.419657422512234 45.014274061990214 17.128874388254488 41 16.894371941272432 23.858075040783035 25.152936378466556 34.09461663947798 42 34.27814029363785 23.215742251223492 27.028955954323003 15.477161500815662 43 15.844208809135399 24.316884176182707 43.48491027732464 16.35399673735726 44 14.02936378466558 43.38295269168026 27.75285481239804 14.834828711256117 45 16.924959216965743 23.39926590538336 43.16884176182708 16.50693311582382 46 33.75815660685155 22.583605220228385 25.86663947797716 17.791598694942902 47 14.294453507340945 24.09257748776509 26.019575856443723 35.59339314845024 48 14.957177814029363 24.286296900489397 26.74347471451876 34.01305057096248 49 31.372349102773246 24.78588907014682 27.875203915171287 15.966557911908646 50 14.590130505709626 23.389070146818923 43.52569331158238 18.49510603588907 51 14.459059855205467 23.738146222086264 27.13368002447232 34.669113898235956 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.5 8 2.0 9 1.0 10 0.0 11 1.0 12 2.0 13 3.0 14 4.0 15 2.0 16 0.0 17 1.0 18 2.0 19 3.5 20 5.0 21 8.5 22 12.0 23 26.5 24 41.0 25 77.5 26 225.5 27 337.0 28 562.0 29 787.0 30 1073.0 31 1359.0 32 1404.0 33 1449.0 34 1293.0 35 1137.0 36 864.0 37 591.0 38 472.5 39 354.0 40 277.5 41 201.0 42 164.0 43 127.0 44 124.5 45 122.0 46 134.5 47 147.0 48 221.0 49 295.0 50 1037.0 51 1779.0 52 983.0 53 187.0 54 173.0 55 159.0 56 125.5 57 92.0 58 97.5 59 103.0 60 94.5 61 86.0 62 73.0 63 60.0 64 53.5 65 47.0 66 45.5 67 44.0 68 39.0 69 34.0 70 34.5 71 35.0 72 28.0 73 21.0 74 23.0 75 18.0 76 11.0 77 9.5 78 8.0 79 12.0 80 16.0 81 11.0 82 6.0 83 4.0 84 2.0 85 2.0 86 2.0 87 2.0 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05097879282218597 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.010195758564437194 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.010195758564437194 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.010195758564437194 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 9808.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.034665579119086 #Duplication Level Percentage of deduplicated Percentage of total 1 73.57935250169798 33.13621533442088 2 11.93117500565995 10.746329526916803 3 5.04867557165497 6.820962479608483 4 2.6488566900611277 4.771615008156607 5 1.9017432646592711 4.282218597063622 6 1.1319900384876613 3.0587275693311584 7 0.7697532261716097 2.426590538336052 8 0.6339144215530903 2.2838499184339316 9 0.5886348200135839 2.3858075040783033 >10 1.7206248585012451 12.489804241435563 >50 0.0 0.0 >100 0.022639800769753225 1.4376019575856445 >500 0.0 0.0 >1k 0.022639800769753225 16.16027732463295 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 1585 16.16027732463295 No Hit TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT 141 1.4376019575856445 RNA PCR Primer, Index 27 (95% over 21bp) TGACAATTCCATTCAATACCAATTGATGATGGTTATTTTTGATTCCATTTG 47 0.47920065252854815 No Hit ATCATGTAATCGTTCAGTTACTTCAAACGTAAAGTAGGCTCAGATTTATTT 44 0.4486133768352365 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 37 0.3772430668841762 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 34 0.3466557911908646 No Hit AGAAATTGAATTCTTGGGTAAACCAATAATGAGTTCTGAAATTGAGACAGT 33 0.3364600326264274 No Hit AAACTAGAGAAAGTTATCAGTATCCAAGTACAAGAAGGTTATGGAACATCA 25 0.25489396411092985 No Hit GAAATACATACTGCATCAATCCTTAGATCAGGGGTTAGCAAACATTTTTGG 25 0.25489396411092985 No Hit GTCAGTAATGCTGTGTAGGATTCTAATTCTTCTCTTGTTTAGGGAAGAGAC 25 0.25489396411092985 No Hit GTGAAATAGGTGCTGAATTTTGGCATTATTCTCATGAAAGATGGATATGGT 24 0.24469820554649263 No Hit ATGTAACATTGTTCTTAAAGAAGGGGCACAGATATAATACAGACAAACTCC 24 0.24469820554649263 No Hit ATGTAACATTGTTCTTAAAAGAAGGGGCACAGATATAATACAGACAAACTC 23 0.23450244698205547 No Hit GTGTATTGTTTTACACATACAGCATGAGTTACTGGTATTTAGCTTGTTTGG 22 0.22430668841761825 No Hit AATAAGGCAAGAGAAAGAAATAAGGGGTATTCAAATAGGAAGAGAGGAAGA 22 0.22430668841761825 No Hit GTATCTCATAGACAACATATAGTCAGGTCTTCTGTTTTGATTACTTCTGAC 21 0.2141109298531811 No Hit CCTTTAATCCATTTTGATTTGACTTTTGTATATGACAAGACATTGGACTAG 21 0.2141109298531811 No Hit AAACAGAAAATCATGGAAAATGAGAAACATCCAGTTGACTACTTGAAAAAT 20 0.20391517128874387 No Hit CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 20 0.20391517128874387 No Hit GGTCTTAACTGAAGAATTGGACAAAGCATTAGCTAGTTTTTGAAATGCTAT 18 0.1835236541598695 No Hit CAGTAATGCTGTGTAGGATTCTAATTCTTCTCTTGTTTAGGGAAGAGACAT 18 0.1835236541598695 No Hit ATTCAATTCCGTTCTATTCCATTCTTTTTCATTCCATTCCATTCCATTCCA 18 0.1835236541598695 No Hit GACATTTAGTGCTATAAATTTTCCTCCTAACACTGCTTTAGCTGCATCCCA 17 0.1733278955954323 No Hit TACCAATGTCTTTTATCACAGTGGCTAGAACGGTAACTAAGACAGTACATA 17 0.1733278955954323 No Hit TCAGTTTTCTTACCATATTCCATGTCCTAGAGTGGATATTTCTAAATTTCC 17 0.1733278955954323 No Hit ACCTTATATGAAAAATGAAATGCCATGGATTAGTGAGTTAGTTCAACTACT 16 0.1631321370309951 No Hit TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT 16 0.1631321370309951 No Hit ACATGAAACTCTTACACATTTTCTTTCAAGATTTCGCCTAGAAGTGATACA 16 0.1631321370309951 No Hit ATCATGGAAAATGAGAAACATCCACTTGACGATTTGAAAAATGACGAAATC 16 0.1631321370309951 No Hit TATATGCACTAGGAGAGGTAAATTTTTTAAAGTACTCATGCAGATTTGAAG 16 0.1631321370309951 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 16 0.1631321370309951 RNA PCR Primer, Index 27 (95% over 21bp) GGATTTTTCTCATTTTCCATGATTTTCAGTTTTCTTGCCATATTCCATGTC 15 0.15293637846655792 No Hit GTACATAAGTTAAAGTCCTAATGGATTTGTACCGTCCTCCCATTTTGTTTT 15 0.15293637846655792 No Hit ATCTCTTTTAGTTCAAGCCTATCTAAAAAGTTACTAATGGAGCTAGAGAAG 15 0.15293637846655792 No Hit GGGTAGAGAATCTTAAAAAACATTTTCTGGCACAAGTTACATTAGCTAAAA 15 0.15293637846655792 No Hit ACTTTCTATAGTCGAGGTTGTAGATCTTAGTAATTCTCTCTCTTCCTTTAA 15 0.15293637846655792 No Hit GACTAAGGGAGATACAGTGAAAATTTTTAAAAATGGGGTCATTTCAAGAGA 14 0.14274061990212072 No Hit ACATTTAGGGATACAATGTACAAACAAAGAAACCATCTCCATATTGTGTTA 14 0.14274061990212072 No Hit AATACATGGTGCAACTGTGATTCAAATACTAAGTTCCTCCAAGGAACTATT 14 0.14274061990212072 No Hit TATTTTTTCTCCAGTCTTGTCTAAACATTTTCCTGAAGGTGTTTTTTCATT 13 0.13254486133768353 No Hit CAGTGATATTTGCATTAAAACTTATACTTCCATCTTTCCAAAAATAGCGGG 13 0.13254486133768353 No Hit AGAGAGAAAAAAAAGAGAGGGTAAGATTATGAGAGTATGAAAGGTGTTTAT 13 0.13254486133768353 No Hit TTTCAAAACTGCTCTGTAAAAAGAAAGGTTCATCTCTGTTAGTTGAATACA 13 0.13254486133768353 No Hit GATAAAGAAAATGTGGTACTTAAACACAATGGATACTATTCAGCCATAAAA 13 0.13254486133768353 No Hit ATAAAGACGCACATAGACTGAAAACAGAAAGATGGAAAAAGATATTCCAAG 12 0.12234910277324632 No Hit TGGATGGAGTGTTCTATAAATGTCGATTAAATCCTACTGGTGGATTTCTCA 12 0.12234910277324632 No Hit GTCATATACTGTAAAAAATGTCAAACTCTCTGACAATTCCCTTAATGAGAA 12 0.12234910277324632 No Hit GAAAGAAAGAAAGAAGGAAAGAAAGAAAGGGAAAGAGAGAGAGAGAAAGAA 12 0.12234910277324632 No Hit ATGGGGAGGAAACTGAAAATCACGGAAAATGAGAAATAAACACTTTAGGAT 12 0.12234910277324632 No Hit GATCAATGTACTTTTCCTTTATCTTCTTCTTTTTCTTCTTGTCACCATCCT 12 0.12234910277324632 No Hit ATATTTATATGGCCCAAACAAATCATATTTCCAAAATCATGTGTACCTTTG 12 0.12234910277324632 No Hit AGTGTATGCTATGAAAACACACAGGCTATAAAAATGCTTAAAGGATACAGC 12 0.12234910277324632 No Hit ATCTTCCTGTGTGAAGTTTATATTCAGGTCTTCTGTCCATTTTTCTAAATT 11 0.11215334420880912 No Hit TATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCTT 11 0.11215334420880912 RNA PCR Primer, Index 27 (100% over 21bp) GATTTATTAGTTAGGCATTTCCAAACTTTCTCCATCGATTGTTGTTTATGA 11 0.11215334420880912 No Hit TTCTTGTAATGTCTTTAATCAGTTTTGGTATCAGGGTAATATTGACCTATG 11 0.11215334420880912 No Hit ATACTGGAGTTTGTCTGTATTATATCTGTGCCCCTTCTTTTAAGAACAATG 11 0.11215334420880912 No Hit CAATACATACTTGGTTCTTTAAATAACAATTGTGACAAACAGCAGAAAGAA 11 0.11215334420880912 No Hit GTAATCTATTTTGATGTAGACGATGAAATCTTAGTTCAAAGACTAACAGGA 11 0.11215334420880912 No Hit GTATAGAAATAACACTGCAATAAGTGGACACTGTCAACATGTGCAGTATTC 11 0.11215334420880912 No Hit GAGAAAACTCAGTGGAATAATGTAGAGAACCTCACTAGGTTTGAAAGACCG 11 0.11215334420880912 No Hit TGTAAAGAGACCAAAAACAGTTTTATTGCTTTACAAGCCAGTAACGAACAC 11 0.11215334420880912 No Hit ACTCAGTGTATTAAAAACAACGATTTTTAATGTTGCCTGGATTTTGGTTCT 11 0.11215334420880912 No Hit GTTCTAAGTTGATAACTAGTAAATCTGGGTGATAGGTAGATGAGAGTCTAT 11 0.11215334420880912 No Hit CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 11 0.11215334420880912 No Hit AAATTACCTTATCAGAACTTTGGTTGTTCTAGCAAATATTTTTTGCACAGT 11 0.11215334420880912 No Hit CAATTATGAAGAGAAAGTGAAAGACAAGGAGGAATGTTTAGAAAAATCAGA 11 0.11215334420880912 No Hit ATTTTATCTGATACCGTTATTCAATTTTGATATTGCATAGGGTGTGCCAAC 11 0.11215334420880912 No Hit TACAAATACAAACCTCACAGATCTTATGAAGCAATCACAATTGAGACTCCA 11 0.11215334420880912 No Hit TTTTTTTCCTTTTCTTTCTGTCTATGAAGTTGCTGTTTATTTTTTTTTGGC 11 0.11215334420880912 No Hit ATTCATAATTGCTCAAAACTTGAATCAACCCAGATGTCCTTCAATAGATAA 11 0.11215334420880912 No Hit CTTTACAAACAGAGTGTTTCCTAACTGCTCTATGAAAAGAAAAGTTAAACT 10 0.10195758564437193 No Hit ATGTGCATTCAACTTGCAGAGTTAAAACTTTCTTTTCATTTAGCAGCTTGG 10 0.10195758564437193 No Hit AGGGAACATTGTAAAGTAACAATTCTTGATATCACGTGCCTCTTATGATCC 10 0.10195758564437193 No Hit GTACATACCATGTGAGTTCTTTTGTGATTGGATTACCTTACTCAGGATGAT 10 0.10195758564437193 No Hit TTTAATGACATTATTGATGGTTTTGTTGATGTTTCTTTCTTTCTGCATAGT 10 0.10195758564437193 No Hit TTTGAGTCAAGCATATCTTCTTACTGAAAGAACAAATTTATAAGCATTGAT 10 0.10195758564437193 No Hit TCTCATGTATATGCAAATGTTTCAAAATCCAAAATTATCCAAAATCTGAAA 10 0.10195758564437193 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.010195758564437194 16 0.0 0.0 0.010195758564437194 17 0.0 0.0 0.020391517128874388 18 0.0 0.0 0.020391517128874388 19 0.0 0.0 0.030587275693311582 20 0.0 0.0 0.030587275693311582 21 0.0 0.0 0.030587275693311582 22 0.0 0.0 0.040783034257748776 23 0.0 0.0 0.040783034257748776 24 0.0 0.0 0.050978792822185974 25 0.0 0.0 0.050978792822185974 26 0.0 0.0 0.050978792822185974 27 0.0 0.0 0.061174551386623165 28 0.0 0.0 0.061174551386623165 29 0.0 0.0 0.07137030995106036 30 0.0 0.0 0.07137030995106036 31 0.0 0.0 0.1733278955954323 32 0.0 0.0 0.28548123980424145 33 0.0 0.0 0.5097879282218597 34 0.0 0.0 0.632137030995106 35 0.0 0.0 0.8360522022838499 36 0.0 0.0 1.1317292006525286 37 0.0 0.0 1.6007340946166395 38 0.0 0.0 2.2124796084828713 39 0.0 0.0 2.722267536704731 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 215 0.0 41.860466 45 ATGCCGT 215 0.0 41.860466 44 TATCTCG 210 0.0 41.785713 36 ATCCTAT 200 0.0 41.625 32 TCCTATC 200 0.0 41.625 33 GATCCTA 200 0.0 41.625 31 ATCTCGT 220 0.0 40.909092 37 TATGCCG 220 0.0 40.909092 43 TCTCGTA 220 0.0 40.909092 38 CGTATGC 220 0.0 40.909092 41 TCGTATG 220 0.0 40.909092 40 CTCGTAT 220 0.0 40.909092 39 CCTATCT 210 0.0 40.714283 34 CTAGATC 205 0.0 40.609756 28 AGATCCT 205 0.0 40.609756 30 TAGATCC 205 0.0 40.609756 29 GAGACTA 205 0.0 40.609756 24 AGCCCAC 225 0.0 40.0 17 GTATGCC 225 0.0 40.0 42 ATCTCCG 225 0.0 40.0 10 >>END_MODULE