FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_19.34100000006fc8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_19.34100000006fc8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9808
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC158516.16027732463295No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT1411.4376019575856445RNA PCR Primer, Index 27 (95% over 21bp)
TGACAATTCCATTCAATACCAATTGATGATGGTTATTTTTGATTCCATTTG470.47920065252854815No Hit
ATCATGTAATCGTTCAGTTACTTCAAACGTAAAGTAGGCTCAGATTTATTT440.4486133768352365No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC370.3772430668841762No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC340.3466557911908646No Hit
AGAAATTGAATTCTTGGGTAAACCAATAATGAGTTCTGAAATTGAGACAGT330.3364600326264274No Hit
AAACTAGAGAAAGTTATCAGTATCCAAGTACAAGAAGGTTATGGAACATCA250.25489396411092985No Hit
GAAATACATACTGCATCAATCCTTAGATCAGGGGTTAGCAAACATTTTTGG250.25489396411092985No Hit
GTCAGTAATGCTGTGTAGGATTCTAATTCTTCTCTTGTTTAGGGAAGAGAC250.25489396411092985No Hit
GTGAAATAGGTGCTGAATTTTGGCATTATTCTCATGAAAGATGGATATGGT240.24469820554649263No Hit
ATGTAACATTGTTCTTAAAGAAGGGGCACAGATATAATACAGACAAACTCC240.24469820554649263No Hit
ATGTAACATTGTTCTTAAAAGAAGGGGCACAGATATAATACAGACAAACTC230.23450244698205547No Hit
GTGTATTGTTTTACACATACAGCATGAGTTACTGGTATTTAGCTTGTTTGG220.22430668841761825No Hit
AATAAGGCAAGAGAAAGAAATAAGGGGTATTCAAATAGGAAGAGAGGAAGA220.22430668841761825No Hit
GTATCTCATAGACAACATATAGTCAGGTCTTCTGTTTTGATTACTTCTGAC210.2141109298531811No Hit
CCTTTAATCCATTTTGATTTGACTTTTGTATATGACAAGACATTGGACTAG210.2141109298531811No Hit
AAACAGAAAATCATGGAAAATGAGAAACATCCAGTTGACTACTTGAAAAAT200.20391517128874387No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC200.20391517128874387No Hit
GGTCTTAACTGAAGAATTGGACAAAGCATTAGCTAGTTTTTGAAATGCTAT180.1835236541598695No Hit
CAGTAATGCTGTGTAGGATTCTAATTCTTCTCTTGTTTAGGGAAGAGACAT180.1835236541598695No Hit
ATTCAATTCCGTTCTATTCCATTCTTTTTCATTCCATTCCATTCCATTCCA180.1835236541598695No Hit
GACATTTAGTGCTATAAATTTTCCTCCTAACACTGCTTTAGCTGCATCCCA170.1733278955954323No Hit
TACCAATGTCTTTTATCACAGTGGCTAGAACGGTAACTAAGACAGTACATA170.1733278955954323No Hit
TCAGTTTTCTTACCATATTCCATGTCCTAGAGTGGATATTTCTAAATTTCC170.1733278955954323No Hit
ACCTTATATGAAAAATGAAATGCCATGGATTAGTGAGTTAGTTCAACTACT160.1631321370309951No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT160.1631321370309951No Hit
ACATGAAACTCTTACACATTTTCTTTCAAGATTTCGCCTAGAAGTGATACA160.1631321370309951No Hit
ATCATGGAAAATGAGAAACATCCACTTGACGATTTGAAAAATGACGAAATC160.1631321370309951No Hit
TATATGCACTAGGAGAGGTAAATTTTTTAAAGTACTCATGCAGATTTGAAG160.1631321370309951No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC160.1631321370309951RNA PCR Primer, Index 27 (95% over 21bp)
GGATTTTTCTCATTTTCCATGATTTTCAGTTTTCTTGCCATATTCCATGTC150.15293637846655792No Hit
GTACATAAGTTAAAGTCCTAATGGATTTGTACCGTCCTCCCATTTTGTTTT150.15293637846655792No Hit
ATCTCTTTTAGTTCAAGCCTATCTAAAAAGTTACTAATGGAGCTAGAGAAG150.15293637846655792No Hit
GGGTAGAGAATCTTAAAAAACATTTTCTGGCACAAGTTACATTAGCTAAAA150.15293637846655792No Hit
ACTTTCTATAGTCGAGGTTGTAGATCTTAGTAATTCTCTCTCTTCCTTTAA150.15293637846655792No Hit
GACTAAGGGAGATACAGTGAAAATTTTTAAAAATGGGGTCATTTCAAGAGA140.14274061990212072No Hit
ACATTTAGGGATACAATGTACAAACAAAGAAACCATCTCCATATTGTGTTA140.14274061990212072No Hit
AATACATGGTGCAACTGTGATTCAAATACTAAGTTCCTCCAAGGAACTATT140.14274061990212072No Hit
TATTTTTTCTCCAGTCTTGTCTAAACATTTTCCTGAAGGTGTTTTTTCATT130.13254486133768353No Hit
CAGTGATATTTGCATTAAAACTTATACTTCCATCTTTCCAAAAATAGCGGG130.13254486133768353No Hit
AGAGAGAAAAAAAAGAGAGGGTAAGATTATGAGAGTATGAAAGGTGTTTAT130.13254486133768353No Hit
TTTCAAAACTGCTCTGTAAAAAGAAAGGTTCATCTCTGTTAGTTGAATACA130.13254486133768353No Hit
GATAAAGAAAATGTGGTACTTAAACACAATGGATACTATTCAGCCATAAAA130.13254486133768353No Hit
ATAAAGACGCACATAGACTGAAAACAGAAAGATGGAAAAAGATATTCCAAG120.12234910277324632No Hit
TGGATGGAGTGTTCTATAAATGTCGATTAAATCCTACTGGTGGATTTCTCA120.12234910277324632No Hit
GTCATATACTGTAAAAAATGTCAAACTCTCTGACAATTCCCTTAATGAGAA120.12234910277324632No Hit
GAAAGAAAGAAAGAAGGAAAGAAAGAAAGGGAAAGAGAGAGAGAGAAAGAA120.12234910277324632No Hit
ATGGGGAGGAAACTGAAAATCACGGAAAATGAGAAATAAACACTTTAGGAT120.12234910277324632No Hit
GATCAATGTACTTTTCCTTTATCTTCTTCTTTTTCTTCTTGTCACCATCCT120.12234910277324632No Hit
ATATTTATATGGCCCAAACAAATCATATTTCCAAAATCATGTGTACCTTTG120.12234910277324632No Hit
AGTGTATGCTATGAAAACACACAGGCTATAAAAATGCTTAAAGGATACAGC120.12234910277324632No Hit
ATCTTCCTGTGTGAAGTTTATATTCAGGTCTTCTGTCCATTTTTCTAAATT110.11215334420880912No Hit
TATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCTT110.11215334420880912RNA PCR Primer, Index 27 (100% over 21bp)
GATTTATTAGTTAGGCATTTCCAAACTTTCTCCATCGATTGTTGTTTATGA110.11215334420880912No Hit
TTCTTGTAATGTCTTTAATCAGTTTTGGTATCAGGGTAATATTGACCTATG110.11215334420880912No Hit
ATACTGGAGTTTGTCTGTATTATATCTGTGCCCCTTCTTTTAAGAACAATG110.11215334420880912No Hit
CAATACATACTTGGTTCTTTAAATAACAATTGTGACAAACAGCAGAAAGAA110.11215334420880912No Hit
GTAATCTATTTTGATGTAGACGATGAAATCTTAGTTCAAAGACTAACAGGA110.11215334420880912No Hit
GTATAGAAATAACACTGCAATAAGTGGACACTGTCAACATGTGCAGTATTC110.11215334420880912No Hit
GAGAAAACTCAGTGGAATAATGTAGAGAACCTCACTAGGTTTGAAAGACCG110.11215334420880912No Hit
TGTAAAGAGACCAAAAACAGTTTTATTGCTTTACAAGCCAGTAACGAACAC110.11215334420880912No Hit
ACTCAGTGTATTAAAAACAACGATTTTTAATGTTGCCTGGATTTTGGTTCT110.11215334420880912No Hit
GTTCTAAGTTGATAACTAGTAAATCTGGGTGATAGGTAGATGAGAGTCTAT110.11215334420880912No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC110.11215334420880912No Hit
AAATTACCTTATCAGAACTTTGGTTGTTCTAGCAAATATTTTTTGCACAGT110.11215334420880912No Hit
CAATTATGAAGAGAAAGTGAAAGACAAGGAGGAATGTTTAGAAAAATCAGA110.11215334420880912No Hit
ATTTTATCTGATACCGTTATTCAATTTTGATATTGCATAGGGTGTGCCAAC110.11215334420880912No Hit
TACAAATACAAACCTCACAGATCTTATGAAGCAATCACAATTGAGACTCCA110.11215334420880912No Hit
TTTTTTTCCTTTTCTTTCTGTCTATGAAGTTGCTGTTTATTTTTTTTTGGC110.11215334420880912No Hit
ATTCATAATTGCTCAAAACTTGAATCAACCCAGATGTCCTTCAATAGATAA110.11215334420880912No Hit
CTTTACAAACAGAGTGTTTCCTAACTGCTCTATGAAAAGAAAAGTTAAACT100.10195758564437193No Hit
ATGTGCATTCAACTTGCAGAGTTAAAACTTTCTTTTCATTTAGCAGCTTGG100.10195758564437193No Hit
AGGGAACATTGTAAAGTAACAATTCTTGATATCACGTGCCTCTTATGATCC100.10195758564437193No Hit
GTACATACCATGTGAGTTCTTTTGTGATTGGATTACCTTACTCAGGATGAT100.10195758564437193No Hit
TTTAATGACATTATTGATGGTTTTGTTGATGTTTCTTTCTTTCTGCATAGT100.10195758564437193No Hit
TTTGAGTCAAGCATATCTTCTTACTGAAAGAACAAATTTATAAGCATTGAT100.10195758564437193No Hit
TCTCATGTATATGCAAATGTTTCAAAATCCAAAATTATCCAAAATCTGAAA100.10195758564437193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2150.041.86046645
ATGCCGT2150.041.86046644
TATCTCG2100.041.78571336
ATCCTAT2000.041.62532
TCCTATC2000.041.62533
GATCCTA2000.041.62531
ATCTCGT2200.040.90909237
TATGCCG2200.040.90909243
TCTCGTA2200.040.90909238
CGTATGC2200.040.90909241
TCGTATG2200.040.90909240
CTCGTAT2200.040.90909239
CCTATCT2100.040.71428334
CTAGATC2050.040.60975628
AGATCCT2050.040.60975630
TAGATCC2050.040.60975629
GAGACTA2050.040.60975624
AGCCCAC2250.040.017
GTATGCC2250.040.042
ATCTCCG2250.040.010
Produced by FastQC (version 0.11.2)