FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_18.34100000006fbb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_18.34100000006fbb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403906
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC9690.23990730516506314No Hit
GTAAATATACAGTTGGATGACATTCTGATACTACAAAGTTACTTTTCTGGC4150.10274667868266378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC1600.040.77900722
TCTCGTA1650.039.5432838
TATCTCG1650.036.8161636
CGTATGC1850.036.48447841
CTCGTAT1800.036.24800539
TATGCCG1800.036.24800543
CGAGACT1800.036.24800523
ATCTCCG1750.036.00247610
GCCCACG1900.035.5243618
TCGTATG1900.035.5243640
CGAGCCC1850.035.27269415
ATGCCGT1850.035.2683344
CCCACGA1900.034.34021419
TGCCGTC1900.034.34021445
CACGAGA1950.033.45969821
GTATGCC1950.033.45969842
GAGACTA1900.033.16017524
ACTAGAT1850.032.84009627
CCACGAG2000.032.62320720
ATCTCGT2050.030.73001737
Produced by FastQC (version 0.11.2)